Biological State Marker for Alcohol Consumption

Wurst, Friedrich Martin; Thon, Natasha; Weinmann, Wolfgang; Yegles, Michel; Preuss, Ulrich W.

doi:10.1007/978-88-470-5322-9_140

Cited by 1 publication

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“…post-mortem analysis. In comparison to state markers (generally used for the assessment of heavy drinking) such as carbohydrate-deficient transferrin (CDT), gamma-glutamyl transferase (γ-GT) or the measurement of the mean corpuscular erythrocyte volume (MCV), direct alcohol markers have a higher selectivity and specificity (2). By investigation of direct alcohol markers within different matrices, evidence of both, short-and long-term consumption can be obtained for high and low doses of alcohol.…”

Section: Introductionmentioning

confidence: 99%

Formation of phosphatidylethanol from endogenous phosphatidylcholines in animal tissues from pig, calf, and goat

Luginbühl

Willem

Schürch

et al. 2018

Forensic Science International

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In the presence of alcohol, phosphatidylcholine (PC) is transformed to the direct alcohol biomarker phosphatidylethanol (PEth). This reaction is catalyzed by the enzyme phospholipase D (PLD) and dependent on substrate availability. As recent methods have solely focused on the determination of PEth, information about the PC composition was generally missing. To address this issue and monitor PC (16:0/18:1 and 16:0/18:2) and PEth (16:0/18:1 and 16:0/18:2) simultaneously, a reversed phase LC-MS/MS method based on a C8 core-shell column, coupled to a Sciex 5500 QTrap instrument was developed. By application of polarity switching, at first, PC was measured in ESI positive SRM mode, while PEth was determined at a later stage in ESI negative SRM mode. The PEth method was validated for human blood samples to show its robustness and subsequently applied for the investigation of systematic in vitro PEth formation in animal tissue samples (brain, kidney, liver, and blood) from a pig, a calf, and a goat. Homogenized tissue was incubated at 37°C with varying ethanol concentrations from 1 to 7g/kg (determined by HS-GC-FID) for 5h, whereby a sample was taken every 30min. For all tissue samples, an increase in PEth was measurable. PEth concentrations formed in blood remained below the LLOQ, in agreement with literature. Data analysis of Michaelis-Menten kinetics and PC within the tissue provided a detailed insight about PEth formation, as the occurrence of PEth species can be linked to the observed PC composition. The results of this study show that PEth formation rates vary from tissue to tissue and among different species. Furthermore, new recommendations for PEth analysis are presented.

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Section: Introductionmentioning

confidence: 99%