Biological Study of protease produced by clinical isolates of Staphylococcus aureus

Introduction and Aim: Thyroid eye illness is widely recognized as one of the most significant concerns confronting the medical profession today. The thyroid-related ophthalmopathy also known as Graves’ ophthalmopathy is an autoimmune disorder that cannot be reversed and hence, research pertaining to the identification of novel markers that can explain both the prognosis and the recovery of the condition is needed. In this study, we aimed to investigate the IL-38 gene expression levels among Graves’ ophthalmopathy patients in Iraq. Methodology: The TSH levels were measured with an enzyme-linked immunosorbent assay (ELISA). The level of IL-38 gene expression in patient blood samples and normal healthy controls was measured qualitatively using RT-PCR. Results: Patients who were diagnosed with Graves’ ophthalmopathy were observed to have abnormally low levels of thyrotropin (TSH) in their serum. In these patients, the interleukin IL-38 gene expression was observed to be significantly greater compared to healthy controls (P<0.01), the tendency of which continued even after one year of therapy with anti-thyroid drugs. Conclusion: The findings of this study indicate that the IL-38 transcript is important in the autoimmune response. The identification of IL-38 expression levels could contribute in the early clinical diagnosis and treatment of thyroid eye disease

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“…There were two identical reactions that were carried out. PCR applied in different area (11)(12)(13)(14)(15)(16)(17).…”

Section: Quantitative Real-time Pcr (Qrt-pcr)mentioning

confidence: 99%

IL-38 gene expression: A new player in Graves’ ophthalmopathy patients in Iraq

Jawad¹,

Numan²,

Ahmed³

et al. 2023

Biomedicine

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“…An increase in the size of the sample will provide results that are easier to distinguish in terms of the effect that the ACE-2 gene has on COVID-19. The molecular approach was used to diagnose many pathogens, including : Proteus vulgaris, 31,32 Clostridium perfringens, 33 Staphylococcus auras, 34,35,36 Brucella melitensis, 37 Pseudomonas spp, 38 Toxoplasma spp 39,40 and SARS-Cov-2. 41,42 Also, Chronic Myeloid Leukemia, 43 Adenocarcinoma, 44,45 and another cancers 46 ,were considered severe genetic illnesses and diagnostic by PCR was suggested.…”

Section: Frequency and Distribution Of Ace-2 Gene Polymorphism And Su...mentioning

confidence: 99%

Genetic Association of Angiotensin-converting enzyme 2 ACE-2 (rs2285666) Polymorphism with the Susceptibility of COVID-19 Disease in Iraqi Patients

2023

TJNPR

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Significant risks to human health are posed by the 2019 coronavirus illness (COVID-19). SARS coronavirus type 2 receptor, also known as the major enzyme in the renin-angiotensin system (RAS), angiotensin-converting enzyme 2 (ACE-2), connects COVID-19 and RAS. This study was conducted with the intention of determining whether or not RAS gene polymorphisms and ACE-2 (G8790A) play a part in the process of predicting susceptibility to infection with COVID-19. In this study 127 participants, 67 of whom were deemed by a physician to be in a severe state of illness, and 60 of whom were categorized as "healthy controls" .The genetic study included an extraction of genomic DNA from blood samples of each covid 19 patients and healthy controls, then amplification the site of SNP (rs2285666) Within the ACE2 gene by using specific primers, sequencing PCR products, and genotyping to detect the role of the ACE-2 gene (rs2285666) in the incidence of COVID-19. ACE-2 (rs2285666) is statistically associated to COVID-19. The COVID-19 group had 65.67 %of individuals with the wild-type homozygous genotype (GG) and 20% in the control group, while the control group had 63.33% of individuals with the mutant genotype (AA). Consequently, the wild-type homozygous (GG) and allele (G) may be considered a risk factor (etiological fraction E. F) for COVID-19 in Iraqi patients, whereas the mutant homozygous (AA) and allele (A) may be considered a protective factor (preventive fraction). The findings of the present study reveal that carriers of the GG genotype of ACE2 (rs2285666) are substantially more susceptible to COVID-19.

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“…DNA extracted was checked for its purity by measuring its absorbance at 260/280nm using a nanodrop. DNA extracted was subjected to amplification of SNP rs1927911 of the TLR4 gene using PCR, this technique used in the medicine area (17)(18)(19). The primers (Macrogen, Korea) given in Table 1.…”

Section: Dna Extraction and Polymerase Chain Reactionmentioning

confidence: 99%

Study of the association of toll like-4 receptor gene polymorphism (rs1927911) and susceptibility to urinary tract infection among Iraqi patients

Allami¹,

Abdullah²,

Al-Ani³

et al. 2023

Biomedicine

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Introduction and Aim: Toll-like receptors (TLRs) are cell innate immune receptors that explore microbial molecules. A quantity of single-nucleotide polymorphisms (SNPs) within the TLR genes have been related with altered susceptibility to infectious, inflammatory, and allergic diseases suggesting: This study was conducted to investigate the relationship between genetic polymorphism in TLR4 gene (rs1927911) with susceptibility to UTIs in a sample of Iraqi patients. Materials and Methods: Blood samples were collected from 220 patients who had UTIs and 69 healthy subjects as control group, during the period from October 2021 to March 2022, at the Rheumatology Department of Clinic Consultant at Al-Yarmouk Hospital. The genetic study included an extraction of genomic DNA and PCR technique, to detect the role of TLR4 gene in the variation site (rs1927911) as a risk factor for the incidence of UTIs. Results: The results of genotype distribution and allele frequency of rs1927911 indicated, CC (18.6 %), CT (70 %) and TT (11.4 %) among the patients while 57.9 % for CC and 26.1%; 16% for CT, TT genotypes respectively of healthy control. Heterozygous (CT) may be considered as a risk factor among UTIs patients. The results revealed significant differences between UTIs patients and healthy controls in serum levels TLR4, IL-6 IL-2, TNF-alpha and IL-10 at P 0.005. In addition, the data found a highly significant difference of serum level of TLR4 protein with patients who have a carrier CT genotype. Conclusion: our findings indicated that the carriers of CT genotype of TLR4 (rs1927911) were significantly more prone to UTIs.

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Biological Study of protease produced by clinical isolates of Staphylococcus aureus

Cited by 11 publications

References 36 publications

IL-38 gene expression: A new player in Graves’ ophthalmopathy patients in Iraq

IL-38 gene expression: A new player in Graves’ ophthalmopathy patients in Iraq

Genetic Association of Angiotensin-converting enzyme 2 ACE-2 (rs2285666) Polymorphism with the Susceptibility of COVID-19 Disease in Iraqi Patients

Study of the association of toll like-4 receptor gene polymorphism (rs1927911) and susceptibility to urinary tract infection among Iraqi patients

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