Biological Tissue Imaging with a Position and Time Sensitive Pixelated Detector

Jungmann, Julia H.; Smith, Donald F.; MacAleese, Luke; Klinkert, Ivo; Visser, J.; Heeren, Ron M. A.

doi:10.1007/s13361-012-0444-5

Cited by 36 publications

(33 citation statements)

References 42 publications

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“…The advantages of a pixelated detector have been previously demonstrated using a MALDI ion microscope. 26,28,29 However, this technology and its benefits have not yet been applied for SIMS imaging.…”

Section: Introductionmentioning

confidence: 99%

Microscope mode secondary ion mass spectrometry imaging with a Timepix detector

Kiss

Jungmann

Smith

et al. 2013

Review of Scientific Instruments

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In-vacuum active pixel detectors enable high sensitivity, highly parallel time-and space-resolved detection of ions from complex surfaces. For the first time, a Timepix detector assembly was combined with a secondary ion mass spectrometer for microscope mode secondary ion mass spectrometry (SIMS) imaging. Time resolved images from various benchmark samples demonstrate the imaging capabilities of the detector system. The main advantages of the active pixel detector are the higher signal-to-noise ratio and parallel acquisition of arrival time and position. Microscope mode SIMS imaging of biomolecules is demonstrated from tissue sections with the Timepix detector.

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Section: Introductionmentioning

confidence: 99%

Microscope mode secondary ion mass spectrometry imaging with a Timepix detector

Kiss

Jungmann

Smith

et al. 2013

Review of Scientific Instruments

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“…32 In another report, a novel chevron multichannel plate (MCP) detector capable of resolving both position and time-of-flight information was coupled to a TOF ion microscope to collect pixels of 740 nm × 740 nm from mouse testis. 33 The potential for collecting the entire mouse testis (5.4 mm × 5.4 mm) was estimated at 23 min, limited by the rate of transfer of data to the computer. These instruments show a strong potential to routinely achieve cellular level imaging within minutes and represent prototypes of the next generation of imaging MS instruments.…”

Section: Experimental Considerationsmentioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization Imaging Mass Spectrometry: In Situ Molecular Mapping

2013

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Matrix-assisted laser desorption ionization imaging mass spectrometry (IMS) is a relatively new imaging modality that allows mapping of a wide range of biomolecules within a thin tissue section. The technology uses a laser beam to directly desorb and ionize molecules from discrete locations on the tissue that are subsequently recorded in a mass spectrometer. IMS is distinguished by the ability to directly measure molecules in situ ranging from small metabolites to proteins, reporting hundreds to thousands of expression patterns from a single imaging experiment. This article reviews recent advances in IMS technology, applications, and experimental strategies that allow it to significantly aid in the discovery and understanding of molecular processes in biological and clinical samples.

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“…The Medipix/Timepix family has found its way into high performance MS for imaging and diagnostic purposes of TOFMS instruments [22,30]. Ions exiting the mass analyzer can be detected on Timepix chip by placing microchannel plates (MCPs) in front of the read-out chip [31].…”

Section: Theorymentioning

confidence: 99%

“…Previously, the use of the Timepix detector with other MS technologies such as secondary ion mass spectrometry (SIMS) [21] and a TOF instrument [22,23] has been demonstrated for MSI with a spatial resolution of few micrometers. In this paper, the implementation of a Timepix detector system on a QMS with a cooling octopole ion guide is presented.…”

Section: Introductionmentioning

confidence: 99%

Experimental Investigation of the 2D Ion Beam Profile Generated by an ESI Octopole-QMS System

Syed

Eijkel

Kistemaker

et al. 2014

J. Am. Soc. Mass Spectrom.

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In this paper, we have employed an ion imaging approach to investigate the behavior of ions exiting from a quadrupole mass spectrometer (QMS) system that employs a radio frequency octopole ion guide before the QMS. An in-vacuum active pixel detector (Timepix) is employed at the exit of the QMS to image the ion patterns. The detector assembly simultaneously records the ion impact position and number of ions per pixel in every measurement frame. The transmission characteristics of the ion beam exiting the QMS are studied using this imaging detector under different operating conditions. Experimental results confirm that the ion spatial distribution exiting the QMS is heavily influenced by ion injection conditions. Furthermore, ion images from Timepix measurements of protein standards demonstrate the capability to enhance the quality of the mass spectral information and provide a detailed insight in the spatial distribution of different charge states (and hence different m/z) ions exiting the QMS.

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Biological Tissue Imaging with a Position and Time Sensitive Pixelated Detector

Cited by 36 publications

References 42 publications

Microscope mode secondary ion mass spectrometry imaging with a Timepix detector

Microscope mode secondary ion mass spectrometry imaging with a Timepix detector

Matrix-Assisted Laser Desorption Ionization Imaging Mass Spectrometry: In Situ Molecular Mapping

Experimental Investigation of the 2D Ion Beam Profile Generated by an ESI Octopole-QMS System

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