Biological transformation of 3β-hydroxy-5-androsten-17-one with the mushroom Pleurotus ostreatus (JACQ. ex FR.) KUMM.

Thoa, Huynh Kim; Šašek, V.; Budêšínský, Miloś; Jablonský, Ivan; Eignerova, L.; Chan, Nguyen Gia; Procházka, Ž.

doi:10.1135/cccc19780336

Cited by 3 publications

(2 citation statements)

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“…This activity was detected only in strains of Trametes and Stereum. Previously, 7a-hydroxylation had been described for Amanita, Armillaria (Akhrem & Titow 1970) and Pleurotus (Thoa et al 1978).…”

Section: Transformation Of Dhea By Mycelial Fungimentioning

confidence: 97%

“…However, the data on 7(a/b)-hydroxylase activity towards DHEA is limited. Activity has been reported for representatives of Amanita, Armillaria (Akhrem & Titow 1970), Botryodiplodia, Colletotrichum (Romano et al 2006), Fusarium (Defaye et al 1978;Cotillon et al 1997;Kolek 1999;Wilson et al 1999), Fusidium (Dodson et al 1959), Mucor (Madyastha & Joseph 1995), Pleurotus (Thoa et al 1978), and Rhizopus (Dodson et al 1959;Choudhary et al 2003). It was shown that 7a-hydroxylase of Fusarium moniliforme was an inducible microsomal enzyme belonging to the cytochrome P-450 superfamily (Cotillon et al 1997).…”

Section: Introductionmentioning

confidence: 96%

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Screening of mycelial fungi for 7α- and 7β-hydroxylase activity towards dehydroepiandrosterone

Lobastova

Gulevskaya

Sukhodolskaya

et al. 2007

Biocatalysis and Biotransformation

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In total, 481 fungal strains were screened for the ability to carry out 7(a/b)-hydroxylation of dehydroepiandrosterone (DHEA, 3b-hydroxy-5-androsten-17-one). Representatives of 31 genera of 15 families and 9 orders of ascomycetes, 17 genera of 9 families and 2 orders of zygomycetes, 2 genera of two families and 2 orders of basidiomycetes, and 14 genera of mitosporic fungi expressed 7(a/b)-hydroxylase activity. The majority of strains were able to introduce a hydroxyl group to position 7a. Active strains selectively producing 3b,7a-dihydroxy-5-androsten-17-one were found among Actinomucor, Backusella, Benjaminiella, Epicoccum, Fusarium, Phycomyces and Trichothecium, with the highest yield of 1.25 and 1.9 g L (1 from 2 and 5 g L (1 DHEA, respectively, reached with F. oxysporum. Representatives of Acremonium, Bipolaris, Conidiobolus and Curvularia formed 3b,7b-dihydroxy-5-androsten-17-one as a major product from DHEA. The structures of the major steroid products were confirmed by thin-layer chromatography (TLC), gas chromatography (GC), mass spectra (MS), and 1 H-NMR analyses.

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Section: Transformation Of Dhea By Mycelial Fungimentioning

confidence: 97%

Section: Introductionmentioning

confidence: 96%

Screening of mycelial fungi for 7α- and 7β-hydroxylase activity towards dehydroepiandrosterone

Lobastova

Gulevskaya

Sukhodolskaya

et al. 2007

Biocatalysis and Biotransformation

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ChemInform Abstract: STEROIDS. PART CXCIX. BIOLOGICAL TRANSFORMATION OF 3β‐HYDROXY‐5‐ANDROSTEN‐17‐ONE WITH THE MUSHROOM PLEUROTUS OSTREATUS

Thoa¹,

Šašek²,

Budêšínský³

et al. 1978

Chemischer Informationsdienst

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Pleurotus mushrooms. Part II. Chemical composition, nutritional value, post‐harvest physiology, preservation, and role as human food

Bano

Rajarathnam

Steinkraus

1988

Critical Reviews in Food Science and Nutrition

168

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The fruit bodies of Pleurotus species as a class of "Edible Fungal Foods" have been discovered to have definite nutritive and medicinal values. They are a good source of nonstarchy carbohydrates, dietary fiber (that can help in reducing the plasma cholesterol), most of the essential amino acids, minerals and vitamins of B group, and folic acid (necessary to counteract pernicious anaemia) in particular. Considering the essential amino acid index, biological value, in vitro digestibility, nutritional index, and protein score, Pleurotus species fall between high grade vegetables and low grade meats. Fractions of water-soluble polysaccharides are reported to possess antitumor activity. The physiological processes such as changes in water content, respiratory rate, texture, color, and activities of enzymes like proteases and polyphenol oxidases during the after-harvest life are delineated. The problems and prospects of processing the fruit bodies by various methods are discussed. Potentialities for production and consumption of the fruit bodies in different parts of the world are brought out.

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Biological transformation of 3β-hydroxy-5-androsten-17-one with the mushroom Pleurotus ostreatus (JACQ. ex FR.) KUMM.

Cited by 3 publications

References 0 publications

Screening of mycelial fungi for 7α- and 7β-hydroxylase activity towards dehydroepiandrosterone

Screening of mycelial fungi for 7α- and 7β-hydroxylase activity towards dehydroepiandrosterone

ChemInform Abstract: STEROIDS. PART CXCIX. BIOLOGICAL TRANSFORMATION OF 3β‐HYDROXY‐5‐ANDROSTEN‐17‐ONE WITH THE MUSHROOM PLEUROTUS OSTREATUS

Pleurotus mushrooms. Part II. Chemical composition, nutritional value, post‐harvest physiology, preservation, and role as human food

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