Biologically Derived Collagen Membranes

Nishihara, Tomio; Rubin, Albert L.; Stenzel, Kurt H.

doi:10.1007/978-1-4615-6555-0_14

Cited by 3 publications

(2 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The platelet-poor plasma was stored in 1.0-ml portions in plastic at -20'C. Plastic or silicone-coated glass equipment was used throughout to prevent Hageman factor activation (6 (14). Calf skin was placed in 10% sodium chloride solution for 12 hr at 4°C and then washed with tap water.…”

Section: Methodsmentioning

confidence: 99%

Studies on the Interaction between Collagen and a Plasma Kallikrein-Like Activity EVIDENCE FOR A SURFACE-ACTIVE ENZYME SYSTEM

Harpel¹

1972

J. Clin. Invest.

View full text Add to dashboard Cite

A B S T R A C T This study has demonstrated that collagen particles, after exposure to platelet-poor human plasma and subsequent washing, generate a kinin-like agent when incubated with prekinin substrate. The binding of kinin-generating activity to collagen in the plasma collagen incubation mixture occurs rapidly, whereas the loss of this activity in the incubation mixture occurs relatively slowly. The Hageman factor appeared to be necessary for the surface-bound kiningenerating activity, as this activity was absent in collagen exposed to Hageman factor-deficient plasma. These studies have partially characterized the plasma-derived enzymatic activity bound to collagen. Incubation of collagen with plasma caused a concentration-dependent reduction in the kinin-producing activity which was generated by the addition of ellagic acid, a known activator of plasma kallikrein. The kinin-inducing activity bound to collagen is inhibited by soybean trypsin inhibitor, Trasylol, serum C1 inactivator and the plasma a2-macroglobulin, but not by lima bean trypsin inhibitor. An eluate prepared from plasma-treated collagen, when compared with purified plasma kallikrein, shared a similar inhibitor profile. Selective chemical blockage of the free carboxyl groups on the collagen molecule, or heat denaturation, inactivated the ability of the collagen to generate kinin-like activity after incubation with plasma. Removal of the collagen telopeptides or blockage of the free amino groups failed to affect the collagen-plasma interaction. The binding of This study was presented in part at the 44th Scientific

show abstract

Section: Methodsmentioning

confidence: 99%

Studies on the Interaction between Collagen and a Plasma Kallikrein-Like Activity EVIDENCE FOR A SURFACE-ACTIVE ENZYME SYSTEM

Harpel¹

1972

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…Collagen has been used in many biomedical applications such as in the dialysis membrane of an artificial kidney (7,(22)(23)(24), an artificial corneal membrane (8, 9), as vitreous body (10-12), in skin and blood vessels (13,14), and as a surgical hemostatic agent (15). In 1969, Chvapil et al .…”

mentioning

confidence: 99%

Use of collagen-hydroxyethylmethacrylate hydrogels for cell growth.

Civerchia-Perez¹,

Faris²,

Lapointe³

et al. 1980

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Collagen-hydroxyethylmethacrylate hydrogels were prepared by polymerizing monomeric hydroxyethylmethacrylate in the presence of various concentrations of soluble native collagen. The resulting transparent hydrogels were evaluated as substrata for growth of IMR-90 human embryonic lung fibroblasts. Without collagen no significant growth occurred, whereas a dose-response curve expressing maximal cell growth against collagen concentration could be constructed quite readily by the use of appropriate hydrogels. The method allows for quantification of the collagen contribution to cell growth and, in a more general sense, provides the foundation for a relatively easy procedure to probe mechanisms of cell adhesion and cell differentiation. Among the many synthetic polymers presently used as biomaterials are those known as hydrogels. The term "hydrogel" refers to a broad class of polymeric materials that are swollen extensively in water but that do not dissolve in water (1). They have been used in a wide variety of biomedical applications and may be synthesized from monomers or monomers mixed with polymers. Hydrogels are attractive as biomaterials; they are highly permeable to water, ions, and small molecules (1). Hydrogels such as poly(2-hydroxyethylmethacrylate) (HEMA) and other synthetic polymers are relatively nontoxic and well tolerated when implanted in vivo (2, 3) or when added to an actively metabolizing tissue-culture system (4-6).The implantation of a biological material such as collagen results in good tissue tolerance both in vivo (7-15) and in vitro (16)(17)(18)(19)(20)(21). Collagen has been used in many biomedical applications such as in the dialysis membrane of an artificial kidney (7,(22)(23)(24), an artificial corneal membrane (8, 9), as vitreous body (10-12), in skin and blood vessels (13,14), and as a surgical hemostatic agent (15). In 1969, Chvapil et al. .(25) described good tissue tolerance in vivo of a collagen-hydrophilic polymer that they had constructed from a calf-hide collagen sponge immersed in HEMA monomer, allowing polymerization to occur on the sponge surface. They attributed the biocompatibility of this material to the porosity of the sponge, which permitted the ingrowth of blood vessels. In 1977, Shimizu et al. (26) reported high tissue compatibility of laminar copolymers of bovine collagen and various synthetic polymers that they had constructed by applying plasma discharge and y-irradiation to effect crosslinkage between a layer of collagen coated onto a layer of synthetic polymer. More recent studies involving collagen-cell surface interactions have focused on the role of fibronectin (27,28).In a study by Folkman and Moscona (29), poly(HEMA) coating was used as a means of preventing fibroblast growth on standard tissue-culture flasks (Falconware). Similarly, a morphologic study of mouse fibroblasts grown on syntheticcoated coverslips found poly(methylmethacrylate) and other polymers to be unfavorable surfaces for cell adhesion and growth (30). In the present communication, w...

show abstract

Biomaterials

Behera

2021

Advanced Materials

View full text Add to dashboard Cite

Biologically Derived Collagen Membranes

Cited by 3 publications

References 4 publications

Studies on the Interaction between Collagen and a Plasma Kallikrein-Like Activity EVIDENCE FOR A SURFACE-ACTIVE ENZYME SYSTEM

Studies on the Interaction between Collagen and a Plasma Kallikrein-Like Activity EVIDENCE FOR A SURFACE-ACTIVE ENZYME SYSTEM

Use of collagen-hydroxyethylmethacrylate hydrogels for cell growth.

Biomaterials

Contact Info

Product

Resources

About