Bioluminescence-based imaging technique for pressure measurement in water

Watanabe, Yasunori; Tanaka, Yasufumi

doi:10.1007/s00348-011-1043-0

Cited by 5 publications

(5 citation statements)

References 14 publications

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“…The objective of this study is to develop AFM as a tool to investigate the relationship between stimulation parameters and physiological sensitivity of individual dinoflagellates, whose in vivo bioluminescence has been used as a reporter of mechanical stress in oceanic and engineering applications (36,(42)(43)(44). Local stimulation using a colloidal probe was applied to single cells of the dinoflagellate Pyrocystis lunula attached to a solid surface.…”

Section: Introductionmentioning

confidence: 99%

Mechanosensitivity of a Rapid Bioluminescence Reporter System Assessed by Atomic Force Microscopy

Tesson

Latz

2015

Biophysical Journal

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Cells are sophisticated integrators of mechanical stimuli that lead to physiological, biochemical, and genetic responses. The bioluminescence of dinoflagellates, alveolate protists that use light emission for predator defense, serves as a rapid noninvasive whole-cell reporter of mechanosensitivity. In this study, we used atomic force microscopy (AFM) to explore the relationship between cell mechanical properties and mechanosensitivity in live cells of the dinoflagellate Pyrocystis lunula. Cell stiffness was 0.56 MPa, consistent with cells possessing a cell wall. Cell response depended on both the magnitude and velocity of the applied force. At the maximum stimulation velocity of 390 μm s(-1), the threshold response occurred at a force of 7.2 μN, resulting in a contact time of 6.1 ms and indentation of 2.1 μm. Cells did not respond to a low stimulation velocity of 20 μm s(-1), indicating a velocity dependent response that, based on stress relaxation experiments, was explained by the cell viscoelastic properties. This study demonstrates the use of AFM to study mechanosensitivity in a cell system that responds at fast timescales, and provides insights into how viscoelastic properties affect mechanosensitivity. It also provides a comparison with previous studies using hydrodynamic stimulation, showing the discrepancy in cell response between direct compressive forces using AFM and those within flow fields based on average flow properties.

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Section: Introductionmentioning

confidence: 99%

Mechanosensitivity of a Rapid Bioluminescence Reporter System Assessed by Atomic Force Microscopy

Tesson

Latz

2015

Biophysical Journal

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“…The bioluminescence of dinoflagellates is stimulated by spatial gradients in fluid velocity rather than absolute flow velocity and can thus serve as a reporter of local fluid shear stress, making it a unique tool for both field and laboratory flow visualization. Based on laboratory studies of bioluminescence stimulation in cylindrical Couette flow , a statistical model has been developed that predicts bioluminescence intensity as a function of fluid shear‐stress level and cell concentration .…”

Section: Introductionmentioning

confidence: 99%

Bubble stimulation efficiency of dinoflagellate bioluminescence

2015

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Dinoflagellate bioluminescence, a common source of bioluminescence in coastal waters, is stimulated by flow agitation. Although bubbles are anecdotally known to be stimulatory, the process has never been experimentally investigated. This study quantified the flash response of the bioluminescent dinoflagellate Lingulodinium polyedrum to stimulation by bubbles rising through still seawater. Cells were stimulated by isolated bubbles of 0.3-3 mm radii rising at their terminal velocity, and also by bubble clouds containing bubbles of 0.06-10 mm radii for different air flow rates. Stimulation efficiency, the proportion of cells producing a flash within the volume of water swept out by a rising bubble, decreased with decreasing bubble radius for radii less than approximately 1 mm. Bubbles smaller than a critical radius in the range 0.275-0.325 mm did not stimulate a flash response. The fraction of cells stimulated by bubble clouds was proportional to the volume of air in the bubble cloud, with lower stimulation levels observed for clouds with smaller bubbles. An empirical model for bubble cloud stimulation based on the isolated bubble observations successfully reproduced the observed stimulation by bubble clouds for low air flow rates. High air flow rates stimulated more light emission than expected, presumably because of additional fluid shear stress associated with collective buoyancy effects generated by the high air fraction bubble cloud. These results are relevant to bioluminescence stimulation by bubbles in two-phase flows, such as in ship wakes, breaking waves, and sparged bioreactors.

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“…Pyrocystis lunula has been used as a biological pressure sensor to measure the impulsive dynamic water pressure produced using weight-drop tests [117] To study lung diseases Te expression of Photinus luciferase has been used to evaluate nuclear factor-κB (NF-κB) in rat models. By detecting the timing, distribution, and intensity, lung activities and infammatory responses have been studied [118] Cancer studies…”

Section: Bioluminescence Imagingmentioning

confidence: 99%

A Comprehensive Exploration of Bioluminescence Systems, Mechanisms, and Advanced Assays for Versatile Applications

Dunuweera,

Ranganathan

2024

Biochemistry Research International

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Bioluminescence has been a fascinating natural phenomenon of light emission from living creatures. It happens when the enzyme luciferase facilitates the oxidation of luciferin, resulting in the creation of an excited-state species that emits light. Although there are many bioluminescent systems, few have been identified. D-luciferin-dependent systems, coelenterazine-dependent systems, Cypridina luciferin-based systems, tetrapyrrole-based luciferins, bacterial bioluminescent systems, and fungal bioluminescent systems are natural bioluminescent systems. Since different bioluminescence systems, such as various combinations of luciferin-luciferase pair reactions, have different light emission wavelengths, they benefit industrial applications such as drug discovery, protein-protein interactions, in vivo imaging in small animals, and controlling neurons. Due to the expression of luciferase and easy permeation of luciferin into most cells and tissues, bioluminescence assays are applied nowadays with modern technologies in most cell and tissue types. It is a versatile technique in a variety of biomedical research. Furthermore, there are some investigated blue-sky research projects, such as bioluminescent plants and lamps. This review article is mainly based on the theory of diverse bioluminescence systems and their past, present, and future applications.

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Bioluminescence-based imaging technique for pressure measurement in water

Cited by 5 publications

References 14 publications

Mechanosensitivity of a Rapid Bioluminescence Reporter System Assessed by Atomic Force Microscopy

Mechanosensitivity of a Rapid Bioluminescence Reporter System Assessed by Atomic Force Microscopy

Bubble stimulation efficiency of dinoflagellate bioluminescence

A Comprehensive Exploration of Bioluminescence Systems, Mechanisms, and Advanced Assays for Versatile Applications

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