2016
DOI: 10.1117/1.nph.3.2.025001
|View full text |Cite
|
Sign up to set email alerts
|

Bioluminescence imaging in live cells and animals

Abstract: Abstract. The use of bioluminescent reporters in neuroscience research continues to grow at a rapid pace as their applications and unique advantages over conventional fluorescent reporters become more appreciated. Here, we describe practical methods and principles for detecting and imaging bioluminescence from live cells and animals. We systematically tested various components of our conventional fluorescence microscope to optimize it for long-term bioluminescence imaging. High-resolution bioluminescence image… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

1
68
1

Year Published

2017
2017
2021
2021

Publication Types

Select...
6
4

Relationship

2
8

Authors

Journals

citations
Cited by 70 publications
(70 citation statements)
references
References 43 publications
1
68
1
Order By: Relevance
“…To explore this possibility, we considered the range of luciferases used for biological research; these include Vargula hilgendorfii (VLuc ( Stacer et al, 2013)). Whereas VLuc requires ATP to produce bioluminescence, limiting it to intracellular use, the others do not (Tung et al, 2016). Among the ATP-independent variants, NanoLuc has been characterized as one of the brightest-emitting luciferase variants (England et al, 2016), and its emission wavelength (450 nm) overlaps with the activation spectrum of LOV.…”
Section: Selection Of Nanoluc As the Luciferase Donor For Lovmentioning
confidence: 99%
“…To explore this possibility, we considered the range of luciferases used for biological research; these include Vargula hilgendorfii (VLuc ( Stacer et al, 2013)). Whereas VLuc requires ATP to produce bioluminescence, limiting it to intracellular use, the others do not (Tung et al, 2016). Among the ATP-independent variants, NanoLuc has been characterized as one of the brightest-emitting luciferase variants (England et al, 2016), and its emission wavelength (450 nm) overlaps with the activation spectrum of LOV.…”
Section: Selection Of Nanoluc As the Luciferase Donor For Lovmentioning
confidence: 99%
“…2A and 2B). As bioluminescence detection generally has a negligible background [17,18], we hypothesized that luciferase-expressing tumor-cell variants may have survived in a dormant state in the caecum of tumor-rejecting mice [19]. Adaptive immune cells, in particular CD4 + and CD8 + T-lymphocytes, are essential for the establishment and maintenance of tumor dormancy [20].…”
Section: In Rejecting Mice Tumor Cells Do Not Survive In a Dormant Stmentioning
confidence: 99%
“…This interpretation is in conflict with our previous observation that direct injection of DTZ into blank mice caused negligible signals, in addition to a plethora of publications which successfully used CTZ or CTZ analogs for in vivo BLI. 17,[22][23][24][25] In this manuscript, we re-investigated the performance of Akaluc and Antares2 bioluminescent reporters for tracking i.v.-injected cells in mice, and by using hydrodynamic transfection and xenograft tumor mouse models. We identified several factors, such as ATP dependency, serum stability, and molecular size, which significantly affected BLI results.…”
Section: Figure 2 Chemical Structures and Bioluminescence Emission Wmentioning
confidence: 99%