Biomacromolecular-based ionic-covalent hydrogels for cell encapsulation: The atelocollagen − Oxidized polysaccharides couples

Luca, Andreea Roxana; Maier, Vasilica; Maier, Stelian Sergiu; Butnaru, Maria; Danu, Maricel; Ibănescu, Constanţa; Pinteala, Mariana; Popa, Marcel

doi:10.1016/j.carbpol.2017.04.046

Cited by 8 publications

(6 citation statements)

References 25 publications

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“…However, the region between 1500–1000 cm −1 undergoes important modifications that are associated with the formation of semi‐IPN system among the polysaccharides and the collagen‐PU matrix. These modifications are related to hydrogen bond interactions and physicochemical associations among the components 24,25,29 …”

Section: Resultsmentioning

confidence: 99%

“…Various strategies involving the formation of semi-IPN/IPN collagen-polysaccharide hydrogels have been reported; such as celluloses derivatized with aldehyde groups, 17,19 thiolated-hyaluronic acid 22 and oxidized-dextran. 23,24 The high presence of -OH groups in the polysaccharide chains promotes the generation of hydrogen bonds among the amino and carboxyl groups of collagen, generating hybrid matrices with high physical crosslinking and high biocompatibility.…”

Section: Appearance and Cross-linking Of Hydrogelsmentioning

confidence: 99%

“…These modifications are related to hydrogen bond interactions and physicochemical associations among the components. 24,25,29 The presence of the hydroxyethyl, hydroxypropyl and hydroxymethyl substituents in the chains of the HEC and HPMC polysaccharides, as well as their glycosidic bonds β-(1-4) are factors that determine the chemical structure of the semi-IPN systems generated with the collagen-PU matrix. While in the Alm free of substituents in its hydrocarbon chain and having glycosidic bonds α-(1-4) and α-(1-8) are also parameters that determine the nature of the semi-IPN system formed.…”

Section: Evaluation Of the Chemical And Crystalline Structurementioning

confidence: 99%

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Novel semi‐interpenetrated networks based on collagen‐polyurethane‐polysaccharides in hydrogel state for biomedical applications

Claudio‐Rizo

Hernandez‐Hernandez

Cano-Salazar

et al. 2020

J of Applied Polymer Sci

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The development of collagen hydrogels with tailored properties for improved applications in biomedicine represents an area of opportunity for materials science. The collagen can form semi-interpenetrated networks (semi-IPN) with various natural and/or synthetic polymers. This work aims the preparation of novel hydrogels generated from a collagen matrix cross-linked with polyurethane (PU), and the subsequent inclusion of polysaccharide chains to form semi-IPN systems with improved properties. The choice of polysaccharides for this purpose is related to their ability to modulate the biocompatibility and the antibacterial capacity in various biomedical strategies. The work

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Section: Resultsmentioning

confidence: 99%

Section: Appearance and Cross-linking Of Hydrogelsmentioning

confidence: 99%

Section: Evaluation Of the Chemical And Crystalline Structurementioning

confidence: 99%

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Novel semi‐interpenetrated networks based on collagen‐polyurethane‐polysaccharides in hydrogel state for biomedical applications

Claudio‐Rizo

Hernandez‐Hernandez

Cano-Salazar

et al. 2020

J of Applied Polymer Sci

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“…The polysaccharide oxidation was performed according to the protocols found in the literature and was described in detail in our previous paper [19,47]. Briefly, a gellan solution was prepared at a concentration of 10 mg/mL by adding the gellan powder in double-distilled water and bringing the mixture at 80°C for complete solubilisation under magnetic stirring, to avoid gelation.…”

Section: Polysaccharide Oxidationmentioning

confidence: 99%

“…The same protocol was used for the oxidation of pullulan except for the solubilisation step which did not require any heating. The oxidation degree was determined using the titrimetric method with hydroxylamine hydrochloride, yielding the same crosslinking potential for both polysaccharides [19,47].…”

Section: Polysaccharide Oxidationmentioning

confidence: 99%

Atelocollagen-based Hydrogels Crosslinked with Oxidised Polysaccharides as Cell Encapsulation Matrix for Engineered Bioactive Stromal Tissue

Luca

Butnaru

Maier

et al. 2017

Tissue Eng Regen Med

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Tissue stroma is responsible for extracellular matrix (ECM) formation and secretion of factors that coordinate the behaviour of the surrounding cells through the microenvironment created. It's inability to spontaneously regenerate makes it a good candidate for research studies such as testing various tissue engineered products capable of replacing the stroma in order to assure normal tissue regeneration and function. In this study, a bioactive stroma was obtained considering two main components: 1) the artificial ECM formed using atelocollagen-oxidized polysaccharides hydrogels in which the polysaccharide compound (oxidised gellan or pullulan) has the role of crosslinker and 2) encapsulated stromal cells (dermal fibroblasts, ovarian theca-interstitial and granulosa cells). The cell-hosting ability of the hydrogels is demonstrated by a good diffusion of globular proteins (albumin) while the fibrillar morphology proves to be optimal for cell adhesion. These structural properties and cytocompatibility of the components maintain good cell viability and cell encapsulation for more than 12 days. Nevertheless, the results indicate some differences favouring the gellan crosslinked hydrogels. Ovarian stromal cells functionality was maintained as indicated by hormone secretion, confirming cell-cell signalling in encapsulated and co-culture conditions. In vivo implantation shows the regenerative potential of the cellpopulated hydrogels as they are integrated into the natural tissue. The possibility of cryopreserving the hydrogel-cell system, while maintaining both cell viability and hydrogel structural integrity underlines the potential of these ready-to-use hydrogels as bioactive stroma for multipurpose tissue regeneration.

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Mimicking In Vivo Tissue Microenvironment for In Vitro Testing – Hydrogels for Cell Encapsulation

et al. 2022

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Biomacromolecular-based ionic-covalent hydrogels for cell encapsulation: The atelocollagen − Oxidized polysaccharides couples

Cited by 8 publications

References 25 publications

Novel semi‐interpenetrated networks based on collagen‐polyurethane‐polysaccharides in hydrogel state for biomedical applications

Novel semi‐interpenetrated networks based on collagen‐polyurethane‐polysaccharides in hydrogel state for biomedical applications

Atelocollagen-based Hydrogels Crosslinked with Oxidised Polysaccharides as Cell Encapsulation Matrix for Engineered Bioactive Stromal Tissue

Mimicking In Vivo Tissue Microenvironment for In Vitro Testing – Hydrogels for Cell Encapsulation

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