Biomapping salmonella serovar complexity in broiler carcasses and parts during processing

Rasamsetti, Surendra; Shariat, Nikki

doi:10.1016/j.fm.2022.104149

Cited by 12 publications

(8 citation statements)

References 65 publications

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“…In 2023, Rasamsetti and Shariat [ 17 ] reported that Salmonella enterica serovars Typhimurium, Infantis, and Kentucky were the most commonly isolated and identified serovars among poultry carcass rinsates collected at commercial broiler processing facilities. Additionally, through CRISPR-seroseq, Rasamsetti and Shariat [ 17 ] demonstrated the re-immersion of S . Infantis after carcass cut up on broiler drumsticks before antimicrobial interventions being applied.…”

Section: Introductionmentioning

confidence: 99%

“…Infantis), which can provide bacterial cells with two to five drug resistance genes [ 18 , 19 ]. However, due to the decrease in the number of Salmonella -positive samples among whole bird carcass rinse samples collected post-chill due to antimicrobial use in the processing facilities, it is difficult to investigate the serovar-specific response to antimicrobials [ 17 ]. Understanding the impact of these processing interventions on emerging Salmonella serovars due to the difference in virulence is increasingly important.…”

Section: Introductionmentioning

confidence: 99%

“…Typhimurium is one of the most common Salmonella spp. isolated from poultry meat [ 17 , 22 ], it is commonly used to demonstrate the efficacy of poultry processing interventions such as PAA. Salmonella Typhimurium has also been examined previously for responses to antimicrobial treatment when adhered to chicken skin [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

“…In 2023, Rasamsetti and Shariat [17] reported that Salmonella enterica serovars Typhimurium, Infantis, and Kentucky were the most commonly isolated and identified serovars among poultry carcass rinsates collected at commercial broiler processing facilities. Additionally, through CRISPR-seroseq, Rasamsetti and Shariat [17] demonstrated the re-immersion of S. Infantis after carcass cut up on broiler drumsticks before antimicrobial interventions being applied. S. Infantis is becoming a significant public health and food safety concern.…”

Section: Introductionmentioning

confidence: 99%

“…All other authors declare no competing interests. The commercial affiliation did not alter the adherence to PLOS ONE policies on sharing data and materials use in the processing facilities, it is difficult to investigate the serovar-specific response to antimicrobials [17]. Understanding the impact of these processing interventions on emerging Salmonella serovars due to the difference in virulence is increasingly important.…”

Section: Introductionmentioning

confidence: 99%

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Mitigating the attachment of Salmonella Infantis on isolated poultry skin with cetylpyridinium chloride

Dittoe,

Olson,

Wythe

et al. 2023

PLoS ONE

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To provide the poultry industry with effective mitigation strategies, the effects of cetylpyridinium chloride (CPC) on the reduction of Salmonella Infantis, hilA expression, and chicken skin microbiota were evaluated. Chicken breast skins (4×4 cm; N = 100, n = 10, k = 5) were inoculated with Salmonella (Typhimurium or Infantis) at 4°C (30min) to obtain 108 CFU/g attachment. Skins were shaken (30s), with remaining bacteria being considered firmly attached. Treatments were applied as 30s dips in 50 mL: no inocula-no-treatment control (NINTC), no treatment control (NTC), tap water (TW), TW+600 ppm PAA (PAA), or TW+0.5% CPC (CPC). Excess fluid was shaken off (30s). Samples were homogenized in nBPW (1 min). Samples were discarded. Salmonella was enumerated and Log10 transformed. Reverse transcriptase-qPCR (rt-qPCR) was performed targeting hilA gene and normalized using the 2-ΔΔCt method. Data were analyzed using one-way ANOVA in RStudio with means separated by Tukey’s HSD (P≤0.05). Genomic DNA of rinsates was extracted, 16S rRNA gene (V4) was sequenced (MiSeq), and data analyzed in QIIME2 (P≤0.05 and Q≤0.05). CPC and PAA affected Salmonella levels differently with CPC being effective against S. Infantis compared to TW (P<0.05). Treatment with CPC on S. Infantis-infected skin altered the hilA expression compared to TW (P<0.05). When inoculated with S. Typhimurium, there was no difference between the microbiota diversity of skins treated with PAA and CPC; however, when inoculated with S. Infantis, there was a difference in the Shannon’s Entropy and Jaccard Dissimilarity between the two treatments (P<0.05). Using ANCOM at the genus level, Brochothrix was significant (W = 118) among skin inoculated with S. Typhimurium. Among S. Infantis inoculated, Yersiniaceae, Enterobacterales, Lachnospiraceae CHKCI001, Clostridia vadinBB60 group, Leuconostoc, Campylobacter, and bacteria were significant (40<W>8). CPC and PAA-treated skins had lowest relative abundance of the genera. In conclusion, CPC mitigated Salmonella Infantis, altered hilA expression, and influenced the chicken skin microbiota.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…In 2023, Rasamsetti and Shariat [17] reported that Salmonella enterica serovars Typhimurium, Infantis, and Kentucky were the most commonly isolated and identified serovars among poultry carcass rinsates collected at commercial broiler processing facilities. Additionally, through CRISPR-seroseq, Rasamsetti and Shariat [17] demonstrated the re-immersion of S. Infantis after carcass cut up on broiler drumsticks before antimicrobial interventions being applied. S. Infantis is becoming a significant public health and food safety concern.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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