Biomarkers of inflammatory external root resorption as a result of traumatic dental injury to permanent teeth in children

Gregorczyk-Maga, Iwona; Kaszuba, Marek; Olszewska, Marta; Lichołai, Sabina; Iwaniec, Teresa; Kościelniak, Dorota; Krzyściak, Wirginia; Jurczak, Anna

doi:10.1016/j.archoralbio.2019.01.003

Cited by 16 publications

(28 citation statements)

References 14 publications

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“…Previous research 18 and a recent study 19 showed that EIRR takes place at the same time as changes in the composition of gingival crevicular fluid (GCF) in terms of inflammatory biomarker concentrations. The ratio of receptor activator of nuclear factor kappa B ligand/osteoprotegerin was statistically higher in subjects with severe root resorption than in the control subjects 18 .…”

Section: Introductionmentioning

confidence: 99%

Prognostic markers of post‐traumatic dental external root resorption in children—a pilot study

Gregorczyk-Maga

Szustkiewicz-Karoń

Kaszuba

et al. 2021

Dental Traumatology

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Background External inflammatory root resorption (EIRR) is a frequent complication of traumatic dental injuries (TDI). The aim of this cohort study was to identify novel non‐invasive markers of post‐traumatic EIRR by assessing the changes in the pulp's blood flow and the composition of gingival sulcus fluid (GCF) in children after dental trauma. Material and methods Thirty‐two children were enrolled and assessed within 48 h of a TDI to their permanent incisors and during an additional three visits over a 6‐months follow up period. During each visit, a radiograph and laser Doppler flow (LDF) measurements of the pulp blood flow were performed on the injured tooth, and the GCF was sampled. The permanent first molar tooth of the same subject served as a control for the LDF measurements and the GCF sampling. Concentrations of IL‐1α in the GCF were measured using enzyme‐linked immunosorbent assays. Radiographs were used to confirm EIRR. Results EIRR occurred in fourteen (43.8%) teeth in 32 patients. The teeth with EIRR showed diminished LDF readings when compared with controls at all visits. The LDF readings of the injured teeth continued to decrease at follow up visits. In the EIRR group, levels of IL‐1α in the GCF of teeth with EIRR were increased and significantly higher than those of the control teeth at the visit when EIRR was diagnosed. The IL‐1α levels were significantly higher in the EIRR group when compared to the injured teeth of patients without EIRR at the last follow‐up visit. Conclusions The occurrence of EIRR after mechanical trauma of permanent teeth with complete root development is accompanied by a significant reduction in the blood flow of the tooth's pulp. It is accompanied by an increase in concentrations of IL‐1α in the GCF of EIRR affected teeth. The results of this pilot study identified the use of IL‐1α in GCF and LDF as non‐invasive markers of EIRR.

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Section: Introductionmentioning

confidence: 99%

Prognostic markers of post‐traumatic dental external root resorption in children—a pilot study

Gregorczyk-Maga

Szustkiewicz-Karoń

Kaszuba

et al. 2021

Dental Traumatology

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“…Root resorption (RR) usually presents cementum and dentin resorption caused by odontoclastic activity, which leads to the mobility and even loss of teeth, thus affecting masticatory function (Gregorczyk‐Maga et al, ). The aetiology of RR is complex.…”

Section: Introductionmentioning

confidence: 99%

NLRP3 inflammasome mediates M1 macrophage polarization and IL‐1β production in inflammatory root resorption

Zhang

Liu

Wan

et al. 2020

J Clinic Periodontology

147

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Aims To explore the involvement of NOD‐like receptor protein 3 (NLRP3) inflammasome and M1 macrophage in root resorption (RR). Methods A rat RR model was established by excessive orthodontic force. After different force‐loading time, the expression levels of NLRP3, caspase‐1, and interleukin‐1β (IL‐1β) and distribution of M1 macrophages were analysed by immunohistochemistry and immunofluorescence staining in vivo. Then, the mechanism of NLRP3 activation was further verified by macrophage and human periodontal ligament cell (hPDLC) co‐culture system in vitro. The production levels of NLRP3, caspase‐1, pro‐caspase‐1, and IL‐1β in M1 macrophages in the co‐culture system were detected by Western blot, and the polarization of CD68+IL‐1β+ M1 macrophages was detected by immunofluorescence staining. Results In the rat RR model, NLRP3, caspase‐1, IL‐1β, and M1 macrophages were expressed in periodontal ligament, mainly concentrated around RR areas. Force‐pre‐treated hPDLCs promoted M1 macrophage polarization and the production of NLRP3, caspase‐1, and IL‐1β in M1 macrophages in co‐culture system. When MCC950, an inhibitor of NLRP3 inflammasome, was added, NLRP3 activation and M1 macrophage polarization were inhibited. Conclusions In periodontal tissues, hPDLCs stimulated by force promoted M1 macrophage polarization and increased IL‐1β production by activating NLRP3 inflammasome in M1 macrophages, thus initiating the occurrence of RR.

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“…A recent study has shown that selected biomarkers such as IL‐1alpha in the gingival sulcus fluid may be suitable biological markers of the EIR in future (Gregorczyk‐Maga et al . 2019). However, more evidence is needed to evaluate whether this approach could be beneficial for detecting EIR at its early stage and for reducing radiological exposure in children.…”

Section: Endodontic Management In Cases Of External Infection‐related Resorption (Eir)mentioning

confidence: 99%

Endodontic management of traumatized permanent teeth: a comprehensive review

et al. 2021

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This comprehensive review will look at the dentinepulp complex and its interaction with the surrounding tissues following TDIs. The literature up to 2020 was reviewed based on several searches on PubMed and the Cochrane Library using relevant terms. In addition to the recently revised guidelines of the International Association of Dental Traumatology, this article aims to provide background information with a focus on endodontic aspects and to gather evidence on which a clinician can make decisions on the choice of the appropriate endodontic approach for traumatized permanent teeth.

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Biomarkers of inflammatory external root resorption as a result of traumatic dental injury to permanent teeth in children

Cited by 16 publications

References 14 publications

Prognostic markers of post‐traumatic dental external root resorption in children—a pilot study

Prognostic markers of post‐traumatic dental external root resorption in children—a pilot study

NLRP3 inflammasome mediates M1 macrophage polarization and IL‐1β production in inflammatory root resorption

Endodontic management of traumatized permanent teeth: a comprehensive review

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