Biomass Microcapsules with Stem Cell Encapsulation for Bone Repair

Abstract

Bone defects caused by trauma, tumor, or osteoarthritis remain challenging due to the lack of effective treatments in clinic. Stem cell transplantation has emerged as an alternative approach for bone repair and attracted widespread attention owing to its excellent biological activities and therapy effect. The attempts to develop this therapeutic approach focus on the generation of effective cell delivery vehicles, since the shortcomings of direct injection of stem cells into target tissues. Here, we developed a novel core-shell microcapsule with a stem cell-laden core and a biomass shell by using all-aqueous phase microfluidic electrospray technology. The designed core-shell microcapsules showed a high cell viability during the culture procedure. In addition, the animal experiments exhibited that stem cell-laden core-shell microcapsules have good biocompatibility and therapeutic effect for bone defects. This study indicated that the core-shell biomass microcapsules generated by microfluidic electrospray have promising potential in tissue engineering and regenerative medicine.

Physical Analysis of the Centrifugal Microencapsulation Process

“…Microencapsulation techniques are used increasingly in industries such as food, cosmetic, pharmaceutical, biomedical, and materials or textile. − A few examples of recent biomedical-oriented microencapsulation research are given in refs − . Certain fields of application require a precise control of the capsule properties (size monodispersity, shape regularity, controlled mechanical properties and porosity, aseptic production, high production rates), which is difficult to satisfy using standard encapsulation technologies such as spray drying or coating.…”

Progress in the application of sustained-release drug microspheres in tissue engineering

“…Yang et al. used microfluidic electrospray technology to prepare biomass-carrying stem cell microcapsules for the treatment of bone defects and applied them in animal experiments [ 69 ]. Studies showed that the microcapsules prepared using this method had good compatibility and therapeutic effects, and had broad development prospects in the tissue engineering and medical fields.…”

Vascularized nanocomposite hydrogel mechanically reinforced by polyelectrolyte-modified nanoparticles

“…Hydrogel materials have good application value in ensuring the activity of growth factors and stem cells. 29,30 Therefore, as a kind of injectable hydrogel, the S1P@MSNs/ AHA/NOCC hydrogel could serve as a drug release system to improve the bioactivity of implants by a simple combination between the hydrogel and other implants (Fig. 7A).…”