Biomass Structure and Its Contributions to Recalcitrance During Consolidated Bioprocessing with Clostridium Thermocellum

Abstract

CHAPTER 4. Lignin Exhibits Recalcitrance Associated Features following the Consolidated Bioprocessing of Natural Variants of Populus trichocarpa 4.1 Introduction 4.2 Experimental Method Biomass and Fermentation Lignin, Cellulose, and Hemicellulose Characterization 4.3 Statistical Analysis 4.4 Results Carbohydrate compositions and fermentation yields Carbohydrate structure Lignin structure 4.5 Discussion Low DP cellulose is utilized before high DP cellulose Cellulose crystallinities and hemicellulose molecular weights are likely minor contributors to recalcitrance during CBP Structural variations in lignin structure are apparent between natural variants 4.6 Conclusion CHAPTER 5.