Biomechanical studies on the effect of iatrogenic dentin removal on vertical root fractures

Ossareh, Arezou; Rosentritt, Martin; Kishen, Anil

doi:10.4103/jcd.jcd_126_18

Cited by 27 publications

(8 citation statements)

References 33 publications

(47 reference statements)

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“…An even wider range of biologically active substances is held back by the absence of secondary growth of the roots. This could contribute to a greater yield of the target metabolic products since it is known that the production activity of substances of secondary origin often increases in the roots characterized by secondary growth [ 18 ]. Various methods of inducing the production of secondary metabolites in cultures of hairy roots and their secretion into the culture medium have not been sufficiently implemented in practice.…”

Section: The Use Of Hairy Root Culture As a Producer Of Biologicalmentioning

confidence: 99%

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Modern Trends in the In Vitro Production and Use of Callus, Suspension Cells and Root Cultures of Medicinal Plants

et al. 2020

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This paper studies modern methods of producing and using callus, suspension cells and root cultures of medicinal plants in vitro. A new solution for natural product production is the use of an alternative source of renewable, environmentally friendly raw materials: callus, suspension and root cultures of higher plants in vitro. The possibility of using hairy root cultures as producers of various biologically active substances is studied. It is proven that the application of the genetic engineering achievements that combine in vitro tissue culture and molecular biology methods was groundbreaking in terms of the intensification of the extraction process of compounds significant for the medical industry. It is established that of all the callus processing methods, suspension and root cultures in vitro, the Agrobacterium method is the most widely used in practice. The use of agrobacteria has advantages over the biolistic method since it increases the proportion of stable transformation events, can deliver large DNA segments and does not require special ballistic devices. As a result of the research, the most effective strains of agrobacteria are identified.

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Section: The Use Of Hairy Root Culture As a Producer Of Biologicalmentioning

confidence: 99%

“…One of the important tasks in the genetic engineering of plants is to obtain plants resistant to viruses since, at present, there are no direct ways to combat viral infections of crops [ 18 ].…”

Section: Genetic Engineering and Biotechnology In The In Vitro Promentioning

confidence: 99%

Modern Trends in the In Vitro Production and Use of Callus, Suspension Cells and Root Cultures of Medicinal Plants

et al. 2020

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“…In the SC model the highest stresses were also found at the orifice level (27.2MPa), however the stresses at the exact apex were higher in the CWC as compared to the SC technique. These recorded maximum stresses in both obturation techniques are less from the amount of stresses required to fracture root dentine (24) .…”

Section: Discussionmentioning

confidence: 83%

Stress Analysis in Root Dentine using the Single Cone Versus the Continuous Wave Obturation Technique: A Finite Element Study

Fakhr

Elmesellawy

2023

Egyptian Dental Journal

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Introduction:The maximum stress distribution in radicular dentin was assessed during root canal obturation while using the continuous wave compaction (CWC) and single cone (SC) techniques. Limited information regarding the stresses induced on root dentine during employing the SC technique is currently present in the literature. Materials and Methods:Two different root canal obturation techniques; the CWC and the SC obturation technique were simulated. Mathematical analysis of the stress distribution patterns and maximum von Mises (VM) stresses after load application to all tooth models were assessed at one, five millimeters from the root apices, at the apex and at the orifice by finite element analysis (FEA) using the Cosmos software package (Solid works, Dassault Systems, Cedex, France). Results:In stage one of the CWC the maximum stresses were expressed at the apex (6.3 MPa), while in the second stage the maximum stresses were at the orifice (29.3 MPa). In the SC technique, the maximum stresses were at the orifice (27.2 MPa). Conclusion:Gentle forces should be employed during compaction in the first stage of the CWC technique, due to the greater force concentration at the apex. The stresses generated during obturation in both the CWC and SC techniques are less than that required to fracture root dentine.

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“…Among these, coronal tissue and structure loss-removal due to previous damage (e.g., caries, traumatic dental injuries, tooth wear, and previous operative procedures), intra-operative procedures (e.g., access cavity preparation, root canal instrumentation, irrigants, medicaments and obturation-related forces plus intracanal post preparation, and subsequent remaining pericervical dentine, as well as postoperative factors [coronal restoration, occlusion, and parafunction]). [ 10 11 20 21 22 23 24 25 ] Therefore, irrigants solutions per se may be considered only as one of the factors associated with accumulative damage during root canal treatment.…”

Section: Discussionmentioning

confidence: 99%

Etidronic acid and ethylenediaminetetraacetic acid associated with sodium hypochlorite have limited effect on the compressive fracture resistance of roots ex vivo

et al. 2020

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Aims: To assess the effect of etidronic acid (EA) mixed with sodium hypochlorite (NaOCl) and two ethylenediaminetetraacetic acid-containing preparations (EDTA and SmearClear) alternated with NaOCl, as a final rinse, on root fracture resistance to a compressive force. Materials and Methods: Seventy-two premolar roots were randomly allocated to the following groups according to the irrigant solutions. For the EA group, 9% EA + 2.5% NaOCl were used throughout the assays ( n = 21). The remaining groups received 2.5% NaOCl during and immediately after chemo-mechanical instrumentation. Intermediately, the roots received 17% EDTA ( n = 19), or SmearClear ( n = 16), or no irrigation (control) ( n = 16) plus a final rinse with 2.5% NaOCl. The specimens were subjected to a vertical compressive force loaded at a crosshead speed of 0.02 mm/s parallel to the long axis of the root until fracture occurred. The results were compared statistically using the one-way analysis of variance for intergroup comparisons. Results: The negative control presented with the highest values, whereas the SmearClear presented with the lowest values, though no significant differences were found when comparing the different groups ( P = 0.82). Conclusions: The use of EA + NaOCl or two EDTA formulations in association with NaOCl does not affect the fracture resistance of previously chemo-mechanically prepared roots.

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Biomechanical studies on the effect of iatrogenic dentin removal on vertical root fractures

Cited by 27 publications

References 33 publications

Modern Trends in the In Vitro Production and Use of Callus, Suspension Cells and Root Cultures of Medicinal Plants

Modern Trends in the In Vitro Production and Use of Callus, Suspension Cells and Root Cultures of Medicinal Plants

Stress Analysis in Root Dentine using the Single Cone Versus the Continuous Wave Obturation Technique: A Finite Element Study

Etidronic acid and ethylenediaminetetraacetic acid associated with sodium hypochlorite have limited effect on the compressive fracture resistance of roots ex vivo

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