Biomedical potential of the reactive oxygen species generation and quenching by fullerenes (C60)

Marković, Zoran M.; Trajkovič, Vladimir

doi:10.1016/j.biomaterials.2008.05.005

Cited by 415 publications

(301 citation statements)

References 159 publications

(260 reference statements)

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“…However, it is known that C60 have an ability to quench and generate ROS (15,16). These discrepancies about genotoxicity of C60 may be caused by a duality of C60 itself.…”

Section: Discussionmentioning

confidence: 99%

Fullerene (C60) Is Negative in the In Vivo Pig-A Gene Mutation Assay

Horibata

Ukai

Naoki

et al. 2011

Genes and Environment

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Carbon nanoparticles, such as carbon nanotubes and fullerene (C 60 ), are potential candidates as leading substances in nanotechnologicalˆelds, but little is known about their safety. Here we examined in vivo genotoxicity of C 60 , by performing the Pig-A gene mutation assay in the peripheral blood of male C57BL/6Cr mice. Mice were given single intraperitoneal injection of 3 mg of C 60 particles in 0.5 mL suspension containing 0.1%-Tween80-saline. As a positive control for the Pig-A gene mutation assay, mice were given a single oral administration of N-nitroso-Nethylurea. At 2 and 8 weeks after treatments, we analyzed CD24-negative and -positive red blood cells in peripheral blood and calculated Pig-A mutant frequencies. As a result, we detected no signiˆcant diŠerences in the mutant frequencies between C 60 treated and non-treated mice, indicating that C 60 is negative for genotoxicity in vivo in the limited target tissues assessed in this study. For the full assessment, we need comprehensive whole body survey on the genotoxicity of C 60 .

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“…However, it is known that C60 have an ability to quench and generate ROS (15,16). These discrepancies about genotoxicity of C60 may be caused by a duality of C60 itself.…”

Section: Discussionmentioning

confidence: 99%

Fullerene (C60) Is Negative in the In Vivo Pig-A Gene Mutation Assay

Horibata

Ukai

Naoki

et al. 2011

Genes and Environment

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“…7,8 The use of nanoparticles in biomedicine is currently an active area of research because nanoparticles hold great promise as novel and effective antioxidants for oxidative stress-related diseases. 9,10 The advantages of using nanoparticles as antioxidants over currently available antioxidants are due to the possibility that they have a versatile surface that can be decorated with active agents, 11 can penetrate the cell membrane, 12 can quench radicals without the need for assistance from other detoxifying molecules, 13 and possess higher physical stability in biological media, 14 thus extending their applications to the treatment of oxidative damage. In this context, there is a considerable number of reports describing that various fullerene (C 60 ) derivatives and surfactant-coated C 60 derivatives modified through either covalent or noncovalent approaches had been reported to be biocompatible and to exhibit properties as powerful antioxidants to decompose ROS generated under oxidative stress in different model systems.…”

Section: Ye Et Almentioning

confidence: 99%

Polyhydroxylated fullerene attenuates oxidative stress-induced apoptosis via a fortifying Nrf2-regulated cellular antioxidant defence system

Ye¹,

Chen²,

Jiang³

et al. 2014

IJN

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Polyhydroxylated derivatives of fullerene C 60 , named fullerenols (C 60 [OH] n ), have stimulated great interest because of their potent antioxidant properties in various chemical and biological systems, which enable them to be used as a new promising pharmaceutical for the future treatment of oxidative stress-related diseases, but the details remain unknown. Nuclear factor erythroid 2-related factor 2 (Nrf2) is a principal transcription factor that regulates expression of several antioxidant genes via binding to the antioxidant response element and plays a crucial role in cellular defence against oxidative stress. In this study we investigated whether activation of the Nrf2/antioxidant response element pathway contributes to the cytoprotective effects of C 60 (OH) 24 . Our results showed that C 60 (OH) 24 enhanced nuclear translocation of Nrf2 and upregulated expression of phase II antioxidant enzymes, including heme oxygenase-1 (HO-1), NAD(P)H: quinine oxidoreductase 1, and γ-glutamate cysteine ligase in A549 cells. Treatment with C 60 (OH) 24 resulted in phosphorylation of p38 mitogen-activated protein kinases (p38 MAPK), extracellular signal-regulated kinases, and c-Jun-N-terminal kinases. By using inhibitors of cellular kinases, we showed that pretreatment of A549 cells with SB203580, a specific inhibitor of p38 MAPK, abolished nuclear translocation of Nrf2 and induction of HO-1 protein induced by C 60 (OH) 24 , indicating an involvement of p38 MAPK in Nrf2/HO-1 activation by C 60 (OH) 24 . Furthermore, pretreatment with C 60 (OH) 24 attenuated hydrogen peroxide-induced apoptotic cell death in A549 cells, and knockdown of Nrf2 by small interfering ribonucleic acid diminished C 60 (OH) 24 -mediated cytoprotection. Taken together, these findings demonstrate that C 60 (OH) 24 may attenuate oxidative stress-induced apoptosis via augmentation of Nrf2-regulated cellular antioxidant capacity, thus providing insights into the mechanisms of the antioxidant properties of C 60 (OH) 24 .

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“…5 For example, nanoparticles may contain toxic materials or ions that directly or indirectly harm the cell through the generation of reactive oxygen species (ROS). Indeed, cytotoxicity due to ROS generation has been reported with a number of different nanoparticles, including titanium dioxide (TiO 2 ), 6 C60 fullerenes, 7 and CeO 2. 8 Independent of composition, nanoparticles may also cause cellular damage due to their ability to adhere to or to pass through cell membranes.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and genotoxicity caused by yttrium oxide nanoparticles in HEK293 cells

Selvaraj¹,

Bodapati²,

Murray³

et al. 2014

IJN

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Background The increased use of engineered nanoparticles (NPs) has caused new concerns about the potential exposure to biological systems and the potential risk that these materials may pose on human health. Here, we examined the effects of exposure to different concentrations (0–50 μg/mL) and incubation times (10 hours, 24 hours, or 48 hours) of yttrium oxide (Y 2 O 3 ) NPs on human embryonic kidney (HEK293) cells. Changes in cellular morphology, cell viability, cell membrane integrity, reactive oxygen species levels, mitochondrial membrane potential, cell death (apoptosis and necrosis), and the DNA damage after NP exposure were compared to the effects seen following incubation with paraquat, a known toxicant. Results The 24-hour inhibitory concentration 50 (IC 50 ) of Y 2 O 3 NPs (41±5 nm in size) in the HEK293 cells was found to be 108 μg/mL. Incubation with Y 2 O 3 NPs (12.25–50 μg/mL) increased the ratio of Bax/Bcl-2, caspase-3 expression and promoted apoptotic- and necrotic-mediated cell death in both a concentration and a time-dependent manner. Decreases in cell survivability were associated with elevations in cellular reactive oxygen species levels, increased mitochondrial membrane permeability, and evidence of DNA damage, which were consistent with the possibility that mitochondria impairment may play an important role in the cytotoxic response. Conclusion These data demonstrate that the Y 2 O 3 NP exposure is associated with increased cellular apoptosis and necrosis in cultured HEK293 cells.

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Biomedical potential of the reactive oxygen species generation and quenching by fullerenes (C60)

Cited by 415 publications

References 159 publications

Fullerene (C60) Is Negative in the In Vivo Pig-A Gene Mutation Assay

Fullerene (C60) Is Negative in the In Vivo Pig-A Gene Mutation Assay

Polyhydroxylated fullerene attenuates oxidative stress-induced apoptosis via a fortifying Nrf2-regulated cellular antioxidant defence system

Cytotoxicity and genotoxicity caused by yttrium oxide nanoparticles in HEK293 cells

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