Biomimetic enzyme-linked immunoassay based on a molecularly imprinted 96-well plate for the determination of triazophos residues in real samples

Abstract

In this study, a direct competitive biomimetic enzyme-linked immune-sorbent assay (BELISA) based on a molecularly imprinted nanomembrane as an artificial antibody was developed for the determination of triazophos in real samples. The imprinted film was directly synthesized on the well surface of a 96-well plate using a dummy molecular template under the conditions of thermal polymerization. The developed BELISA using a hapten of triazophos as an enzyme-labeled probe is much more sensitive, simple, quick, steady and inexpensive than the other instrumental and immuno assay methods. Under optimal conditions, the linear range of the method was 0.001-10 000 mg L À1 with a good regression coefficient of 0.977. The sensitivity (IC 50 ) and the limit of detection (LOD) of BELISA were 428 mg L À1 and 0.001 mg L À1, respectively. This method was performed to detect triazophos in cabbage and apple samples, and showed excellent recovery and relative standard deviations (RSDs) ranging from 70.5 to 119.8% and from 5.2 to 19.7%, respectively. The results correlated well with those obtained using high performance liquid chromatography-tandem mass spectrometry.

A Novel CdSe/ZnS Quantum Dots Fluorescence Assay Based on Molecularly Imprinted Sensitive Membranes for Determination of Triazophos Residues in Cabbage and Apple

“…Herein, the synthesis of the imprinted film was prepared according to our previous work (Hong et al, 2018 ). Briefly, triadimefon (2 mmol) was dissolved in 20 mL of ACN, and then MAA (12 mmol) was added.…”

“…Prior to spiking, LC-MS/MS was used to prove that the samples contained no detectable amount of the tested analyte. The pretreatment process of sample preparation was carried out on the basis of our previous study (Hong et al, 2018 ).…”

Rapid detection of triazophos in cucumber using lateral flow immunochromatographic assay

“…Immunoassays based on antibodies and antigens including ELISA are quite popular because of their high sensitivity and accuracy. In cabbage and apple samples, ELISA had a IC 50 of 0.0001 µg TZP/L (Hong et al, 2018). (Gui et al, 2006) ELISA had an LOD 0.11 ng TZP/mL in PBS buffer.…”

“…Immunoassays based on antibodies and antigens are potentially sensitive methods of TZP. The enzyme-linked immunosorbent assay (ELISA) has been used for TZP detection (Gui et al, 2006;Hong et al, 2018), which is highly specific and require minimum sample preparation, is effective high throughput applications; however, the assay is time consuming (Wu et al, 2020;Xu et al, 2020;Zhang et al, 2019). The lateral flow immunochromatography assay (LFA) is easy, fast (5-10 min), and sensitive (Kong et al, 2019;Li et al, 2020;Wang et al, 2020;Zeng et al, 2020).…”

Bio-barcode detection technology and its research applications: A review

“…Nanomaterials are less commonly used in the detection of small-substances, such as additives in food and pesticide residues, than macromolecules; one of the main reasons is the structure of small molecules, which cannot bind to two antibodies due to steric hindrance. To solve this problem, the double sandwich structure can be replaced with a competition model [94] , [95] , [4] , [96] . The main schematic diagram is shown in Fig.…”