Biomineralizing Proteins with Emphasis on Invertebrate‐mineralized Structures

Treccani, Laura; Khoshnavaz, Saeb; Blank, S.; Roedern, K. von; Schulz, Ulrike; Weiss, Ingrid M.; Mann, Karlheinz; Radmacher, Manfred; Fritz, Monika

doi:10.1002/3527600035.bpol8011

Cited by 12 publications

(16 citation statements)

References 110 publications

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“…Proteins guide nucleation and growth of the mineral phase, and together with polysaccharides, they account for the mechanical properties of most of the polymer composites (Kuo et al, 1996;Treccani et al, 2005). Our study also characterized sugar components of the foraminiferal organic matrices (Tables 5 and 7), and we identified the nature of S. floresiana carbohydrates.…”

Section: Carbohydratesmentioning

confidence: 81%

Biomineralization ofSchlumbergerella floresiana, a significant carbonate‐producing benthic foraminifer

et al. 2014

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Most foraminifera that produce a shell are efficient biomineralizers. We analyzed the calcitic shell of the large tropical benthic foraminifer Schlumbergerella floresiana. We found a suite of macromolecules containing many charged and polar amino acids and glycine that are also abundant in biomineralization proteins of other phyla. As neither genomic nor transcriptomic data are available for foraminiferal biomineralization yet, de novo-generated sequences, obtained from organic matrices submitted to ms blast database search, led to the characterization of 156 peptides. Very few homologous proteins were matched in the proteomic database, implying that the peptides are derived from unknown proteins present in the foraminiferal organic matrices. The amino acid distribution of these peptides was queried against the uniprot database and the mollusk uniprot database for comparison. The mollusks compose a well-studied phylum that yield a large variety of biomineralization proteins. These results showed that proteins extracted from S. floresiana shells contained sequences enriched with glycine, alanine, and proline, making a set of residues that provided a signature unique to foraminifera. Three of the de novo peptides exhibited sequence similarities to peptides found in proteins such as pre-collagen-P and a group of P-type ATPases including a calcium-transporting ATPase. Surprisingly, the peptide that was most similar to the collagen-like protein was a glycine-rich peptide reported from the test and spine proteome of sea urchin. The molecules, identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry analyses, included acid-soluble N-glycoproteins with its sugar moieties represented by high-mannose-type glycans and carbohydrates. Describing the nature of the proteins, and associated molecules in the skeletal structure of living foraminifera, can elucidate the biomineralization mechanisms of these major carbonate producers in marine ecosystems. As fossil foraminifera provide important paleoenvironmental and paleoclimatic information, a better understanding of biomineralization in these organisms will have far-reaching impacts.

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Section: Carbohydratesmentioning

confidence: 81%

Biomineralization ofSchlumbergerella floresiana, a significant carbonate‐producing benthic foraminifer

et al. 2014

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“…The resulting tissue has properties very different from those of the pure minerals themselves. The stiff mineral prevents the organic matrix (proteins, peptides, polysaccharides, lipids) from yielding, while the organic matrix prevents the mineral from cracking (Treccani et al, 2003).…”

Section: Biominerals and Biomineralizationmentioning

confidence: 99%

Principles of demineralization: Modern strategies for the isolation of organic frameworks

Ehrlich

Koutsoukos

Demadis

et al. 2008

Micron

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“…One very important requirement for the outstanding mechanical properties of nacre is the strong contact between the mineral platelets and the organic matrix (see also the reviews [9][10][11][65][66][67]). …”

Section: Organic-inorganic Interface At the Mineral Plateletsmentioning

confidence: 99%

Gastropod nacre: Structure, properties and growth — Biological, chemical and physical basics

Heinemann

Launspach

Gries

et al. 2011

Biophysical Chemistry

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109

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Biomineralizing Proteins with Emphasis on Invertebrate‐mineralized Structures

Abstract: Introduction Historical Outline Proteins Involved in Biomineralization Biosilicates Calcium Carbonate Biomineralization in Invertebrates The Avian Eggshell Patents Outlooks and Perspectives Acknowledgments

Cited by 12 publications

References 110 publications

Biomineralization ofSchlumbergerella floresiana, a significant carbonate‐producing benthic foraminifer

Biomineralization ofSchlumbergerella floresiana, a significant carbonate‐producing benthic foraminifer

Principles of demineralization: Modern strategies for the isolation of organic frameworks

Gastropod nacre: Structure, properties and growth — Biological, chemical and physical basics

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