Biomolecule Use in Tissue Engineering

Depprich, Rita

doi:10.1007/978-3-540-77755-7_11

Cited by 4 publications

(2 citation statements)

References 135 publications

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“…Elles représentent un des sous-groupes de la superfamille transforming growth factor b (TGF-b). Cette superfamille regroupe les BMP et d'autres facteurs de croissance [24]. De nombreux sous-types des BMP ont été identifiés [28].…”

Section: Les Biomoléculesunclassified

Ingénierie du tissu osseux oro-maxillofacial par combinaison de biomatériaux, cellules souches, thérapie génique

Myon

Ferri

Chai

et al. 2011

Revue de Stomatologie et de Chirurgie Maxillo-faciale

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Section: Les Biomoléculesunclassified

Ingénierie du tissu osseux oro-maxillofacial par combinaison de biomatériaux, cellules souches, thérapie génique

Myon

Ferri

Chai

et al. 2011

Revue de Stomatologie et de Chirurgie Maxillo-faciale

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“…The BMPs constitute a family of proteins with the ability to initiate osteoblastic differentiation [ 37 ] and have proven to enhance osteoinductive characteristics of bone graft substitutes (e.g. in combination with autogenous bone blocks or collagenous sponges) [ 38 – 43 ]. rhBMP-2 in particular has shown the highest osteoinductive potential of the BMP family [ 44 ].…”

Section: Introductionmentioning

confidence: 99%

Vertical bone regeneration using rhBMP-2 and VEGF

et al. 2017

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BackgroundSufficient vertical and lateral bone supply and a competent osteogenic healing process are prerequisities for the successful osseointegration of dental implants in the alveolar bone. Several techniques including autologous bone grafts and guided bone regeneration are applied to improve quality and quantity of bone at the implantation site. Depending on the amount of lacking bone one- or two-stage procedures are required. Vertical bone augmentation has proven to be a challenge particularly in terms of bone volume stability. This study focuses on the three dimensional vertical bone generation in a one stage procedure in vivo. Therefore, a collagenous disc-shaped scaffold (ICBM = Insoluble Collagenous Bone Matrix) containing rhBMP-2 (Bone Morphogenetic Protein-2) and/or VEGF (Vascular Endothelial Growth Factor) was applied around the coronal part of a dental implant during insertion. RhBMP-2 and VEGF released directly at the implantation site were assumed to induce the generation of new vertical bone around the implant.MethodsOne hundred eight titanium implants were inserted into the mandible and the tibia of 12 mini pigs. Four experimental groups were formed: Control group, ICBM, ICBM + BMP-2, and ICBM + BMP-2 + VEGF.After 1, 4 and 12 weeks the animals were sacrificed and bone generation was investigated histologically and histomorphometrically.ResultsAfter 12 weeks the combination of ICBM + rhBMP2 + VEGF showed significantly more bone volume density (BVD%), a higher vertical bone gain (VBG) and more vertical bone gain around the implant (PVBG) in comparison to the control group.ConclusionBy using collagenous disc-shaped matrices in combination with rhBMP-2 and VEGF vertical bone can be generated in a one stage procedure without donor site morbidity. The results of the presenting study suggest that the combination of rhBMP-2 and VEGF applied locally by using a collagenous carrier improves vertical bone generation in vivo. Further research is needed to establish whether this technique is applicable in clinical routines.

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Surface Nanocoating with Plant‐Derived Pectins Improves Fibroblast Response In Vitro

Folkert¹,

Mieszkowska²,

Gaber³

et al. 2018

Starch Stärke

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Pectins, plant‐derived polysaccharides, are novel candidates for biomaterial nanocoatings. Their chemical structure imitates the polysaccharides from the extracellular matrix of mammals, providing bio‐specific cell adhesion. It is possible to control the structure of pectin rhamnogalacturonan‐I (RG‐I) by enzymatic modification. RG‐Is has been proposed for surface nanocoating of orthopedic and dental titanium implants due to its effect on osseointegration with stimulation of bone and connective tissue healing. The aim of the current study is to evaluate in vitro, the impact of plant‐derived pectin RG‐I nanocoating on the cellular response of human fibroblasts. In this study, unmodified RG‐I and enzymatically modified RG‐I with low amount of arabinose and high amount of galactose are examined. All in vitro studies are performed on tissue culture polystyrene plates, whose surfaces are coated with unmodified and modified RG‐I. The results show that the nanocoating with pectin RG‐Is increas proliferation, cell metabolic activity, and gene expression of COL1A1, FN1, FGFR1, BMP7, and MMP2 as compared to control surface, without pectin coatings. The results show that nanocoating with RG‐Is has the capacity to improve fibroblast proliferation, differentiation, and extracellular matrix protein production, and may therefore be considered as a potential candidate for further improvement of bone and connective tissue regeneration.

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Biomolecule Use in Tissue Engineering

Cited by 4 publications

References 135 publications

Ingénierie du tissu osseux oro-maxillofacial par combinaison de biomatériaux, cellules souches, thérapie génique

Ingénierie du tissu osseux oro-maxillofacial par combinaison de biomatériaux, cellules souches, thérapie génique

Vertical bone regeneration using rhBMP-2 and VEGF

Surface Nanocoating with Plant‐Derived Pectins Improves Fibroblast Response In Vitro

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