Biomonitoring of 37 trace elements in blood samples from inhabitants of northern Germany by ICP–MS

Following root canal treatment, sealers may contact periapical tissue. The purpose of this study was to evaluate the systemic toxic effects of epoxy resin-based sealers (AH Plus and Obtuseal). Inductively coupled plasma-mass spectrometry (ICP-MS) was used to measure levels of trace elements (beryllium, magnesium, aluminium, calcium, chromium, arsenic and lead) in the brain, kidney and liver of rats. Twenty sterilized polyethylene tubes were then filled with AH Plus and Obtuseal and implanted into the dorsal subcutaneous tissue of 10 rats; three unoperated animals were used as a control group. After 45 days, the rats were sacrificed by cervical dislocation following anaesthesia, and brains, kidneys and livers were removed from all experimental animals. ICP-MS analysis was used to determine levels of trace elements. Data were analysed using KruskalWallis and Connover post hoc tests. No significant differences were found in aluminium and calcium levels, but brains, kidneys and livers showed significantly higher amounts of magnesium and chromium than the corresponding controls. In the kidney and liver samples, arsenic levels were found to be higher than in the control group. Lead was detected at higher levels only in liver samples from the AH Plus group. Beryllium was not detected in any organ. It was concluded that AH Plus and Obtuseal release minimal quantities of trace elements when in contact with subcutaneous tissue, and further studies are needed to understand the systemic effects of these materials.

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“…The well-documented advantages of ICP-MS also include greater sensitivity and selectivity [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Determination of trace elements in kidneys, livers and brains of rats with sealer implants by ICP-MS

Şımşek

Akinci

Alan

et al. 2017

Biotechnology & Biotechnological Equipment

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“…In fact, Ga-substituted protoporphyrin (Ga-PP) is commonly considered to be a good model to study the heme-globin interaction [32]. Furthermore, gallium is not an essential metal and, unlike most of its neighbors in the periodic table, it is not present in the human body at levels exceeding 0.2 ppb [33] (unless the subjects had chronic exposure to this metal [34]), which should minimize interferences in ICP MS and LA-ICP MS measurements.…”

Section: Rationale For Selecting Gallium As a Metal Tracermentioning

confidence: 99%

Evaluation of Gallium as a Tracer of Exogenous Hemoglobin–Haptoglobin Complexes for Targeted Drug Delivery Applications

Kaltashov

2016

J. Am. Soc. Mass Spectrom.

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Abstract. Haptoglobin (Hp) is a plasma glycoprotein that generates significant interest in the drug delivery community because of its potential for delivery of antiretroviral medicines with high selectivity to macrophages and monocytes, the latent reservoirs of human immunodeficiency virus. As is the case with other therapies that exploit transport networks for targeted drug delivery, the success of the design and optimization of Hp-based therapies will critically depend on the ability to accurately localize and quantitate Hp-drug conjugates on the varying and unpredictable background of endogenous proteins having identical structure. In this work, we introduce a new strategy for detecting and quantitating exogenous Hp and Hp-based drugs with high sensitivity in complex biological samples using gallium as a tracer of this protein and inductively coupled plasma mass spectrometry (ICP MS) as a method of detection. Metal label is introduced by reconstituting hemoglobin (Hb) with gallium(III)-protoporphyrin IX followed by its complexation with Hp. Formation of the Hp/Hb assembly and its stability are evaluated with native electrospray ionization mass spectrometry. Both stable isotopes of Ga give rise to an abundant signal in ICP MS of a human plasma sample spiked with the metallabeled Hp/Hb complex. The metal label signal exceeds the spectral interferences' contributions by more than an order of magnitude even with the concentration of the exogenous protein below 10 nM, the level that is more than adequate for the planned pharmacokinetic studies of Hp-based therapeutics.

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“…Ces interférences sont classiquement regroupées en interférences nonspectroscopiques et spectroscopiques. (3,19,20) ou en milieu basique (NH 4 OH à 0,5 ‰) (21,22) ; en effet l'ammoniaque favorise l'hémolyse et la dissolution des protéines et peut minimiser la formation des interférences polyatomiques type ArCl + par capture des ions chlorure, relativement abondants dans les matrices biologiques (10). L'utilisation d'un digesteur micro-ondes peut s'avérer intéressante, dans la mesure où l'on a pu s'affranchir des effets mémoire (24).…”

Section: Difficultés Spécifiques Liées Aux Interférencesunclassified

“…Le dosage des éléments trace et ultra-trace prend toute son importance en biologie médicale pour la famille des métaux. En excluant les domaines de la médecine légale et de la toxicologie professionnelle traités par ailleurs, on peut citer, sans faire une liste fastidieuse, des exemples de métaux d'intérêt clinique dont le dosage gagne à être effectué par ICP-MS : le cas du mercure avec la problématique des amalgames dentaires, du plomb avec la surveillance de la plombémie de l'enfant mineur, du platine et du cuivre libres dans le cadre de suivis thérapeutiques (traitements anticancéreux (13,(19)(20)(21)(22)(23). Le biologiste doit pouvoir alors disposer de valeurs usuelles afin de dépister une concentration anormalement élevée ou à l'inverse une carence élémentaire.…”

Section: Difficultés Spécifiques Liées Aux Interférencesunclassified

Utilisation de l'ICP-MS en biologie clinique

Mœsch

2007

Ann Toxicol Anal

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RÉSUMÉ L'analyse des éléments, métaux ou métalloïdes, dans diverses matrices n'a pas échappé au progrès scientifique et l'utilisation de la spectrométrie de masse inorganique couplée à un plasma induit par haute fréquence (ICP-MS MOTS-CLÉS ICP-MS, biologie, métaux, métalloïdes. SUMMARY The analysis of the elements, metals or metalloids, in various matrices has benefited from scientific progress and the use of inorganic mass spectrometry coupled with plasma induced by high frequency (ICP-MS) is a perfect illustration. ICP-MS, which has only fairly recently been introduced into medical biological analyses, has the advantages related to the association of the two techniques : the inductively coupled plasma gives the extent of the accessible elements associated with a high-level of sensibility whereas mass spectrometry brings also its sensibility (more than sixty elements have a detection limit below 10 μg/L), the extent of its linear dynamic range covering eight orders of magnitude as well as the speed of analysis. Another quality of the ICP-MS is its aptitude for KEY-WORDSICP-MS, biology, metals, metalloids.

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Biomonitoring of 37 trace elements in blood samples from inhabitants of northern Germany by ICP–MS

Cited by 257 publications

References 35 publications

Determination of trace elements in kidneys, livers and brains of rats with sealer implants by ICP-MS

Determination of trace elements in kidneys, livers and brains of rats with sealer implants by ICP-MS

Evaluation of Gallium as a Tracer of Exogenous Hemoglobin–Haptoglobin Complexes for Targeted Drug Delivery Applications

Utilisation de l'ICP-MS en biologie clinique

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