Biophysical evaluation of physiological effects of gilthead sea bream (Sparus aurata) farming using FTIR spectroscopy

Ceylan, Cagatay; Tanrıkul, T. Tansel; Özgener, Hüseyin

doi:10.1016/j.foodchem.2013.08.111

Cited by 9 publications

(9 citation statements)

References 22 publications

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“…Additionally, PAS staining reveals reduced glycogen content in BLT and MLT. Several reports indicate that the ratios of glycogen-tocholesterol and glucose-to-phospholipids in FTIR spectra could be used as biochemical indicators of metabolic activity in different malignancies [23,25,51,68]. Herein, we report that the ratio of glycogen-to-cholesterol exhibits a decreasing trend with progression of PFH, and this agrees with the increasing level in glucose-to-phospholipids ratio.…”

Section: Discussionsupporting

confidence: 87%

“…The ratio of 1760‐1730 cm −1 /2995‐2800 cm −1 corresponds to CO/lipids, which reflects the level of lipid peroxidation . The glucose‐to‐phospholipids ratio was obtained by calculating the intensity ratio of 1038 cm −1 /2926 cm −1 . The intensity ratio of 1121 cm −1 /1545 cm −1 was used to measure the RNA‐to‐Amide II ratio as a potential biomarker to predict cellular gene transcription .…”

Section: Methodsmentioning

confidence: 99%

“…The glycogen‐to‐cholesterol ratio was obtained by calculating the intensity ratio of 1045 cm −1 /1465 cm −1 . The intensity ratios of 1086 cm −1 /1545 cm −1 and 1080 cm −1 /1550 cm −1 were respectively used to measure the ratios of DNA‐to‐Amide II and nucleic acids‐to‐Amide II, which are inversely correlated with the apoptotic index .…”

Section: Methodsmentioning

confidence: 99%

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Fourier transform infrared and Raman‐based biochemical profiling of different grades of pure foetal‐type hepatoblastoma

Duan

Yang

et al. 2019

Journal of Biophotonics

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The biomolecular events resulting from the progression of hepatoblastoma remain to be elucidated. Fourier‐transform infrared (FTIR) and Raman spectroscopies are capable of noninvasively and accurately capturing the biochemical properties of biological tissue from its pathological status. Our aim was to probe critial biomolecular changes of liver accompanying the progression of pure foetal hepatoblastoma (PFH) by FTIR and Raman spectroscopies. Herein, biochemical alterations were both evident in the FTIR spectra (regions of 3100‐2800 cm−1 and 1800‐900 cm−1) and the Raman spectra (region of 1800‐400 cm−1) among normal, borderline and malignant liver tissues. Compared with normal tissues, the ratios of protein‐to‐lipid, α‐helix‐to‐β‐sheet, RNA‐to‐DNA, CH3 methyl‐to‐CH2 methylene, glucose‐to‐phospholipids, and unsaturated‐to‐saturated lipids intensities were significantly higher in malignant tissues, while the ratios of RNA‐to‐Amide II, DNA‐to‐Amide II, glycogen‐to‐cholesterol and Amide I‐to‐Amide II intensities were remarkably lower. These biochemical alterations in the transition from normal to malignant have profound implications not only for cyto‐pathological classification but also for molecular understanding of PFH progression. The successive changes of the spectral characteristics have been shown to be consistent with the development of PFH, indicating that FTIR and Raman spectroscopies are excellent tools to interrogate the biochemical features of different grades of PFH.

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Section: Discussionsupporting

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Fourier transform infrared and Raman‐based biochemical profiling of different grades of pure foetal‐type hepatoblastoma

Duan

Yang

et al. 2019

Journal of Biophotonics

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“…S1) and following the information present in Table 2, which was compiled from different sources (Çakmak, Togan & Severcan, 2006; Çakmak et al, 2003; Ceylan, Tanrikul & Özgener, 2014; Palaniappan, Nishanth & Renju, 2010; Palaniappan & Vijayasundaram, 2008; Palaniappan & Vijayasundaram, 2009; Sánchez-Alonso, Carmona & Careche, 2012). …”

Section: Methodsmentioning

confidence: 99%

“…The characteristic wavelengths absorbed by a specific molecule depend on the energy differences between vibrational states of the molecule, being mostly defined by the presence of particular functional groups, as each functional group tends to display specific vibration modes, thereby conditioning the energy of allowed vibrational transitions (Ellis & Goodacre, 2006). Though the use of FT-IR spectroscopy as a metabolic fingerprinting technology in fish biology and aquaculture research is still incipient, several studies have already pointed out its usefulness in such diverse contexts as the differentiation between wild vs. farmed gilthead seabream (Ceylan, Tanrikul & Özgener, 2014), the study of the effects of estrogens and other pollutants in rainbow trout (Çakmak, Togan & Severcan, 2006; Çakmak et al, 2003), analysis of the effects of zinc and arsenic in indian carp (Palaniappan, Nishanth & Renju, 2010; Palaniappan & Vijayasundaram, 2008; Palaniappan & Vijayasundaram, 2009) and analysis of lipids in frozen hake fillets (Sánchez-Alonso, Carmona & Careche, 2012), underlying the flexibility of this technique in the study of distinct fish tissues within the context of several different biological problems.…”

Section: Introductionmentioning

confidence: 99%

Metabolic fingerprinting of gilthead seabream (Sparus aurata) liver to track interactions between dietary factors and seasonal temperature variations

Silva

Costa

Conceição

et al. 2014

PeerJ

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Farmed gilthead seabream is sometimes affected by a metabolic syndrome, known as the “winter disease”, which has a significant economic impact in the Mediterranean region. It is caused, among other factors, by the thermal variations that occur during colder months and there are signs that an improved nutritional status can mitigate the effects of this thermal stress. For this reason, a trial was undertaken where we assessed the effect of two different diets on gilthead seabream physiology and nutritional state, through metabolic fingerprinting of hepatic tissue. For this trial, four groups of 25 adult gilthead seabream were reared for 8 months, being fed either with a control diet (CTRL, low-cost commercial formulation) or with a diet called “Winter Feed” (WF, high-cost improved formulation). Fish were sampled at two time-points (at the end of winter and at the end of spring), with liver tissue being taken for FT-IR spectroscopy. Results have shown that seasonal temperature variations constitute a metabolic challenge for gilthead seabream, with hepatic carbohydrate stores being consumed over the course of the inter-sampling period. Regarding the WF diet, results point towards a positive effect in terms of performance and improved nutritional status. This diet seems to have a mitigating effect on the deleterious impact of thermal shifts, confirming the hypothesis that nutritional factors can affect the capacity of gilthead seabream to cope with seasonal thermal variations and possibly contribute to prevent the onset of “winter disease”.

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Iodine excess induces hepatic, renal and pancreatic injury in female mice as determined by attenuated total reflection Fourier‐transform infrared spectrometry

Guo

Xia

et al. 2021

J of Applied Toxicology

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Limited knowledge of the long‐term effects of excessive iodine (EI) intake on biomolecular signatures in the liver/pancreas/kidney prompted this study. Herein, following 6 months of exposure in mice to 300, 600, 1200 or 2400 μg/L iodine, the biochemical signature of alterations to the liver/pancreas/kidney was profiled using attenuated total reflection Fourier‐transform infrared (ATR‐FTIR) spectroscopy coupled with principal component analysis‐linear discriminant analysis (PCA‐LDA). Our research showed that serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), serum creatinine (Scr), insulin, blood glucose levels and homeostasis model assessment for insulin resistance (HOMA‐IR) index in the 1200 and 2400 μg/L iodine‐treated groups were significantly increased compared with those in the control group. Moreover, histological analysis showed that the liver/kidney/pancreas tissues of mice exposed to EI treatment displayed substantial morphological abnormalities, such as a loss of hepatic architecture, glomerular cell vacuolation and pancreatic neutrophilic infiltration. Notably, EI treatment caused distinct biochemical signature segregation between EI‐exposed versus the control liver/pancreas/kidney. The main biochemical alterations between EI‐exposed and control groups were observed for protein phosphorylation, protein secondary structures and lipids. The ratios of amide I‐to‐amide II (1674 cm−1/1570 cm−1), α‐helix‐to‐β‐sheet (1657 cm−1/1635 cm−1), glycogen‐to‐phosphate (1030 cm−1/1086 cm−1) and the peptide aggregation (1 630 cm−1/1650 cm−1) level of EI‐treated groups significantly differed from the control group. Our study demonstrated that EI induced hepatic, renal and pancreatic injury by disturbing the structure, metabolism and function of the cell membrane. This finding provides the new method and implication for human health assessment regarding long‐term EI intake.

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Biophysical evaluation of physiological effects of gilthead sea bream (Sparus aurata) farming using FTIR spectroscopy

Cited by 9 publications

References 22 publications

Fourier transform infrared and Raman‐based biochemical profiling of different grades of pure foetal‐type hepatoblastoma

Fourier transform infrared and Raman‐based biochemical profiling of different grades of pure foetal‐type hepatoblastoma

Metabolic fingerprinting of gilthead seabream (Sparus aurata) liver to track interactions between dietary factors and seasonal temperature variations

Iodine excess induces hepatic, renal and pancreatic injury in female mice as determined by attenuated total reflection Fourier‐transform infrared spectrometry

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