Biophysical Foundations Neuron Response to Small Radiation Exposure

Abstract

The purpose of the work is to study the different parts of the brain of rats, subjected to gamma irradiation in doses up to 1 Sievert by morphological methods. The research was made on 270 adult mongrel rats-males weighing 210±10 g, aged 4 months to the beginning of the experiment, irradiated by gamma quanta 60Co range of 1.2 MeV once doses of 10, 20, 50 and 100 szv with a capacity of 50 cGy/H. The material was taken in 1 day, 6, 12,18 and 24 months post-radiation period. The material was fixed in 10 % formalin solution in the mixture Karnau. The authors studied the structural and functional organization of neurocytes on preparations by the method of Nisse. Protein was identified by the method of Bonkheg and nucleic acids have been identified by the methods Brachet J. L. and S.K. Shea. Contents of protein and nucleic acids were determined largest optical density by a computer program Image J. 36 b Wayne Rasband National Institutes of Health, USA. Material was processed statistically. The authors identified three stages of the reaction of nervous system to the radiation factor: early manifestations (from 1 to 30 days); expressed changes (up to 6 months), and recovery. Life expectancy of irradiated animals has no changes, but some fluctuations morphological characteristics may affect the quality of life of the population of the contaminated territories.