Biopolymer-Based Hydrogels for Cartilage Tissue Engineering

Balakrishnan, Biji; Banerjee, Rinti

doi:10.1021/cr100123h

Cited by 500 publications

(402 citation statements)

References 214 publications

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“…Cell sheets are a scaffold-free alternative but are quite difficult to grow and handling them without damaging the delicate sheet is not without challenge [18]. In all cases, there are solutions to the previously stated problems, some of them will be discussed further along in the chapter, and others fall outside of the scope of the chapter but are discussed in other excellent review papers [18,20,[23][24][25][26].…”

Section: Bio Vs Artificialmentioning

confidence: 99%

Plasma Science and Technology - Progress in Physical States and Chemical Reactions

Mieno¹

2016

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Non-thermal plasma technology is one of those techniques that suffer relatively little from diffusion limits, slow kinetics, and complex geometries compared to more traditional liquid-based chemical surface modification techniques. Combined with a lack of solvents, preservation of the bulk properties, and fast treatment times; it is a well-liked technique for the treatment of materials for biomedical applications. In this book chapter, a review will be given on what the scientific community determined to be essential to obtain appropriate scaffolds for tissue engineering and how plasma scientists have used non-thermal plasma technology to accomplish this. A distinction will be made depending on the scaffold fabrication technique, as each technique has its own set of specific problems that need to be tackled. Fabrication techniques will include traditional fabrication methods, rapid prototyping, and electrospinning. As for the different plasma techniques, both plasma activation and grafting/polymerization will be included in the review and linked to the in-vitro/in-vivo response to these treatments. The literature review itself is preceded by a more general overview on cell communication, giving useful insights on how surface modification strategies should be developed.

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Section: Bio Vs Artificialmentioning

confidence: 99%

Plasma Science and Technology - Progress in Physical States and Chemical Reactions

Mieno¹

2016

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“…Hydrogels have very wide applicability in the biomedical and pharmaceutical fields including tissue engineering [2][3][4], diagnostics, and drug delivery [5,6]. Hydrogels are biocompatible because they cause minimal tissue irritation with little cell adherence when in contact with the extracellular matrix due to their hydrophilicity and soft nature [7].…”

Section: Introductionmentioning

confidence: 99%

Biodegradable and Biocompatible PDLLA-PEG1k-PDLLA Diacrylate Macromers: Synthesis, Characterisation and Preparation of Soluble Hyperbranched Polymers and Crosslinked Hydrogels

et al. 2017

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A series of P DL LA-PEG 1k -P DL LA tri-block co-polymers with various compositions, i.e., containing 2-10 lactoyl units, were prepared via ring opening polymerisation of D,L-lactide in the presence of poly (ethylene glycol) (PEG) (M n = 1000 g·mol −1 ) as the initiator and stannous 2-ethylhexanoate as the catalyst at different feed ratios. P DL LA-PEG 1k -P DL LA co-polymers were then functionalised with acrylate groups using acryloyl chloride under various reaction conditions. The diacrylated P DL LA-PEG 1k -P DL LA (diacryl-P DL LA-PEG 1k -P DL LA) were further polymerised to synthesize soluble hyperbranched polymers by either homo-polymerisation or co-polymerisation with poly(ethylene glycol) methyl ether methylacrylate (PEGMEMA) via free radical polymerisation. The polymer samples obtained were characterised by 1 H NMR (proton Nuclear Magnetic Resonance), FTIR (Fourier Transform Infra-red spectroscopy), and GPC (Gel Permeation Chromatography). Moreover, the diacryl-P DL LA-PEG 1k -P DL LA macromers were used for the preparation of biodegradable crosslinked hydrogels through the Michael addition reaction and radical photo-polymerisation with or without poly(ethylene glycol) methyl ether methylacrylate (PEGMEMA, M n = 475 g·mol −1 ) as the co-monomer. It was found that fine tuning of the diacryl-P DL LA-PEG 1k -P DL LA constituents and its combination with co-monomers resulted in hydrogels with tailored swelling properties. It is envisioned that soluble hyperbranched polymers and crosslinked hydrogels prepared from diacryl-P DL LA-PEG 1k -P DL LA macromers can have promising applications in the fields of nano-medicines and regenerative medicines.

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“…Typically, such hydrogel materials can be functionalized or assembled with specific functional groups to impart enhanced biocompatible properties. For example, esterification of hyaluronic acid with benzoic acid results in a water-insoluble scaffold (HYAFF 11) which has been used clinically to treat chronic symptomatic defects in joint surfaces [25,26]. In another study, dicarboxymethyl chitosan conjugated with bone morphogenetic protein-7 demonstrated repair of cartilage lesions [27].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Engineered Peptide-Glycosaminoglycan Microfibrous Hybrid Scaffolds for Potential Applications in Cartilage Tissue Regeneration

Romanelli

Knoll

Santora

et al. 2015

Fibers

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Advances in tissue engineering have enabled the ability to design and fabricate biomaterials at the nanoscale that can actively mimic the natural cellular environment of host tissue. Of all tissues, cartilage remains difficult to regenerate due to its avascular nature. Herein we have developed two new hybrid polypeptide-glycosaminoglycan microfibrous scaffold constructs and compared their abilities to stimulate cell adhesion, proliferation, sulfated proteoglycan synthesis and soluble collagen synthesis when seeded with chondrocytes. Both constructs were designed utilizing self-assembled Fmoc-protected valyl cetylamide nanofibrous templates. The peptide components of the constructs were varied. For Construct I a short segment of dentin sialophosphoprotein followed by Type I collagen were attached to the templates using the layer-by-layer approach. For Construct II, a short peptide segment derived from the integrin subunit of Type II collagen binding protein expressed by chondrocytes was attached to the templates followed by Type II collagen. To both constructs, we then attached the natural polymer N-acetyl glucosamine, chitosan. Subsequently, the glycosaminoglycan chondroitin sulfate was then attached as the final layer. The scaffolds were characterized by Fourier transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), atomic force microscopy and scanning electron microscopy. In vitro culture studies were carried out in the presence of chondrocyte cells for OPEN ACCESSFibers 2015, 3 266 both scaffolds and growth morphology was determined through optical microscopy and scanning electron microscopy taken at different magnifications at various days of culture. Cell proliferation studies indicated that while both constructs were biocompatible and supported the growth and adhesion of chondrocytes, Construct II stimulated cell adhesion at higher rates and resulted in the formation of three dimensional cell-scaffold matrices within 24 h. Proteoglycan synthesis, a hallmark of chondrocyte cell differentiation, was also higher for Construct II compared to Construct I. Soluble collagen synthesis was also found to be higher for Construct II. The results of the above studies suggest that scaffolds designed from Construct II be superior for potential applications in cartilage tissue regeneration. The peptide components of the constructs play an important role not only in the mechanical properties in developing the scaffolds but also control cell adhesion, collagen synthesis and proteoglycan synthesis capabilities.

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Biopolymer-Based Hydrogels for Cartilage Tissue Engineering

Cited by 500 publications

References 214 publications

Plasma Science and Technology - Progress in Physical States and Chemical Reactions

Plasma Science and Technology - Progress in Physical States and Chemical Reactions

Biodegradable and Biocompatible PDLLA-PEG1k-PDLLA Diacrylate Macromers: Synthesis, Characterisation and Preparation of Soluble Hyperbranched Polymers and Crosslinked Hydrogels

Comparison of Engineered Peptide-Glycosaminoglycan Microfibrous Hybrid Scaffolds for Potential Applications in Cartilage Tissue Regeneration

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