Bioprocess development for the production of xylooligosaccharide prebiotics from agro-industrial lignocellulosic waste

Dong, Cheng–Di; Tsai, Mei-Ling; Nargotra, Parushi; Kour, Bhavneet; Chen, Chiu-Wen; Sun, Pei-Pei; Sharma, Vishal

doi:10.1016/j.heliyon.2023.e18316

Cited by 17 publications

(7 citation statements)

References 118 publications

(171 reference statements)

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“…The enzymatic method was used to perform the biocatalytic hydrolysis of corn stover xylan . Briefly, corn stover xylan was extracted with sodium hydroxide (10% w/v) and its content (mg) was calculated by quantifying the xylose obtained from sulfuric acid hydrolysis .…”

Section: Methodsmentioning

confidence: 99%

“…The enzymatic method was used to perform the biocatalytic hydrolysis of corn stover xylan. 39 Briefly, corn stover xylan was extracted with sodium hydroxide (10% w/v) and its content (mg) was calculated by quantifying the xylose obtained from sulfuric acid hydrolysis. 13 The synergistic catalysis mixtures comprising 200 U/g XynG1-1 and 200 U/g rXYLO in 50 mM sodium phosphate buffer (pH 8.0) with 5 g/L substrate were incubated at 50 °C and 180 rpm.…”

Section: Biocatalytic Hydrolysis Of Corn Stover Xylan For the Product...mentioning

confidence: 99%

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Computation-Aided Phylogeny-Oriented Engineering of β-Xylosidase: Modification of “Blades” to Enhance Stability and Activity for the Bioconversion of Hemicellulose to Produce Xylose

Zhang,

Gao,

Song

et al. 2024

J. Agric. Food Chem.

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Hemicellulose is a highly abundant, ubiquitous, and renewable natural polysaccharide, widely present in agricultural and forestry residues. The enzymatic hydrolysis of hemicellulose has generally been accomplished using β-xylosidases, but concomitantly increasing the stability and activity of these enzymes remains challenging. Here, we rationally engineered a βxylosidase from Bacillus clausii to enhance its stability by computation-aided design combining ancestral sequence reconstruction and structural analysis. The resulting combinatorial mutant rXYLO M25I/S51L/S79E exhibited highly improved robustness, with a 6.9-fold increase of the half-life at 60 °C, while also exhibiting improved pH stability, catalytic efficiency, and hydrolytic activity. Structural analysis demonstrated that additional interactions among the propeller blades in the catalytic module resulted in a much more compact protein structure and induced the rearrangement of the opposing catalytic pocket to mediate the observed improvement of activity. Our work provides a robust biocatalyst for the hydrolysis of agricultural waste to produce various high-value-added chemicals and biofuels.

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Section: Methodsmentioning

confidence: 99%

Section: Biocatalytic Hydrolysis Of Corn Stover Xylan For the Product...mentioning

confidence: 99%

Computation-Aided Phylogeny-Oriented Engineering of β-Xylosidase: Modification of “Blades” to Enhance Stability and Activity for the Bioconversion of Hemicellulose to Produce Xylose

Zhang,

Gao,

Song

et al. 2024

J. Agric. Food Chem.

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“…XOS can be produced from a large variety of lignocellulosic feedstocks, therefore making it an excellent candidate for production in a sugar cane biorefinery utilizing 2G feedstock . However, the lack of efficient production methods currently hinders its commercial production . Using the xylose fraction of sugar cane lignocellulose for XOS production can provide a pretreated cellulignin for subsequent enzymatic hydrolysis into sugars for conversion to coproducts such as PDO.…”

Section: Introductionmentioning

confidence: 99%

“…40 However, the lack of efficient production methods currently hinders its commercial production. 41 Using the xylose fraction of sugar cane lignocellulose for XOS production can provide a pretreated cellulignin for subsequent enzymatic hydrolysis into sugars for conversion to coproducts such as PDO.…”

Section: ■ Introductionmentioning

confidence: 99%

Techno-Economic and Environmental Assessments of 1,3-Propanediol and Xylooligosaccharide Production Annexed to a Typical Sugar Mill

van Heerden,

Farzad,

Görgens

2023

ACS Sustainable Chem. Eng.

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“…Sci. 2023, 24, 17110 2 of 21 Xylan, derived from lignocellulosic biomass, can be hydrolysed through the use of exogenous chemicals and enzymatic processes to generate a mixture of xylooligosaccharides (XOSs) and monosaccharides [7,8]. The resulting XOS mixture is recognized as a prebiotic [1].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Xylooligosaccharides Production by Native and Recombinant Xylanase Hydrolysis of Chicken Feed Substrates

Dhaver,

Pletschke,

Sithole

et al. 2023

IJMS

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Poultry production faces several challenges, with feed efficiency being the main factor that can be influenced through the use of different nutritional strategies. Xylooligosaccharides (XOS) are functional feed additives that are attracting growing commercial interest due to their excellent ability to modulate the composition of the gut microbiota. The aim of the study was to apply crude and purified fungal xylanases, from Trichoderma harzianum, as well as a recombinant glycoside hydrolase family 10 xylanase, derived from Geobacillus stearothermophilus T6, as additives to locally produced chicken feeds. A Box–Behnken Design (BBD) was used to optimize the reducing sugar yield. Response surface methodology (RSM) revealed that reducing sugars were higher (8.05 mg/mL, 2.81 mg/mL and 2.98 mg/mL) for the starter feed treated with each of the three enzymes compared to the treatment with grower feed (3.11 mg/mL, 2.41 mg/mL and 2.62 mg/mL). The hydrolysis products were analysed by thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC) analysis and showed that the enzymes hydrolysed the chicken feeds, producing a range of monosaccharides (arabinose, mannose, glucose, and galactose) and XOS, with xylobiose being the predominant XOS. These results show promising data for future applications as additives to poultry feeds.

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Bioprocess development for the production of xylooligosaccharide prebiotics from agro-industrial lignocellulosic waste

Cited by 17 publications

References 118 publications

Computation-Aided Phylogeny-Oriented Engineering of β-Xylosidase: Modification of “Blades” to Enhance Stability and Activity for the Bioconversion of Hemicellulose to Produce Xylose

Computation-Aided Phylogeny-Oriented Engineering of β-Xylosidase: Modification of “Blades” to Enhance Stability and Activity for the Bioconversion of Hemicellulose to Produce Xylose

Techno-Economic and Environmental Assessments of 1,3-Propanediol and Xylooligosaccharide Production Annexed to a Typical Sugar Mill

Optimization of Xylooligosaccharides Production by Native and Recombinant Xylanase Hydrolysis of Chicken Feed Substrates

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