Bioprocess Scale-up for Acetohydroxamic Acid Production by Hyperactive Acyltransferase of Immobilized Rhodococcus Pyridinivorans

Devi, Neena; Patel, Sanjay; Kumar, Pradeep; Singh, Archana; Thakur, Nandita; Lata, Jeevan; Pandey, Deepak; Thakur, Vikram; Chand, Duni

doi:10.1007/s10562-021-03696-4

Cited by 13 publications

(3 citation statements)

References 31 publications

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“…Take PHI-1090 as an example, as shown in Figure 5 and Table S1, in the temperature range of 38-48 • C, the L -lactic acid produced by the immobilized cells was higher than that of the suspended cells owing to the low thermal conductivity of the polyHIPE. In addition, 28.6% of the remaining activity of the immobilized cells was retained at 40 h, which is higher than alginate immobilized cells (55% at 4 h) [47], demonstrating the excellent temperature stability of the PHI immobilized cells. The optical purity of L -lactic acid produced by immobilized P. acidilactici was 99.6 ± 0.1%, which was equivalent to that of L -lactic acid generated by suspended P. acidilactici, indicating that immobilization had no influence on the L -lactic acid generation.…”

Section: Fermentation Process Of Immobilized P Acidilacticimentioning

confidence: 88%

Hierarchical Emulsion-Templated Monoliths (polyHIPEs) as Scaffolds for Covalent Immobilization of P. acidilactici

2023

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The immobilized cell fermentation technique (IMCF) has gained immense popularity in recent years due to its capacity to enhance metabolic efficiency, cell stability, and product separation during fermentation. Porous carriers used as cell immobilization facilitate mass transfer and isolate the cells from an adverse external environment, thus accelerating cell growth and metabolism. However, creating a cell-immobilized porous carrier that guarantees both mechanical strength and cell stability remains challenging. Herein, templated by water-in-oil (w/o) high internal phase emulsions (HIPE), we established a tunable open-cell polymeric P(St-co-GMA) monolith as a scaffold for the efficient immobilization of Pediococcus acidilactici (P. acidilactici). The porous framework’s mechanical property was substantially improved by incorporating the styrene monomer and cross-linker divinylbenzene (DVB) in the HIPE’s external phase, while the epoxy groups on glycidyl methacrylate (GMA) supply anchoring sites for P. acidilactici, securing the immobilization to the inner wall surface of the void. For the fermentation of immobilized P. acidilactici, the polyHIPEs permit efficient mass transfer, which increases along with increased interconnectivity of the monolith, resulting in higher L-lactic acid yield compared to that of suspended cells with an increase of 17%. The relative L-lactic acid production is constantly maintained above 92.9% of their initial relative production after 10 cycles, exhibiting both its great cycling stability and the durability of the material structure. Furthermore, the procedure during recycle batch also simplifies downstream separation operations.

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Section: Fermentation Process Of Immobilized P Acidilacticimentioning

confidence: 88%

Hierarchical Emulsion-Templated Monoliths (polyHIPEs) as Scaffolds for Covalent Immobilization of P. acidilactici

2023

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“…Hyperactive immobilized amidase from Rhodococcus pyridinivorans was utilized to improve acetohydroxamic acid production. In contrast to free cells, calcium-alginate immobilized cells showed high bioconversion of acetamide to acetohydroxamic acid giving 96 % yield [31]. Synthesis of hydroxamic acids through nitrile hydrataseamidase-cascade reaction was reported by Vejvoda and coworkers.…”

Section: Enzymatic Synthesismentioning

confidence: 98%

Enzymatic Catalysts for Hydroxamic Acid Formation: A Mini‐Review

Singh

2023

ChemBioEng Reviews

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In recent years, biocatalysts have emerged as crucial tool in organic synthesis, particularly for the production of drug intermediates and precursors, e.g., the synthesis of hydroxamic acids. Traditionally, hydroxamic acids were synthesized using organic chemistry methods. However, with the growing emphasis on sustainable and environment‐friendly practices, the chemical industry has increasingly turned towards green synthesis approaches. The significance of hydroxamic acids in medicinal chemistry has also contributed to the changing trends. Following the approval of certain hydroxamic acids as histone deacetylase (HDAC) inhibitors for cancer treatment by the Food and Drug Administration (US‐FDA), there has been a renewed focus on their synthesis and the development of derivatives with improved properties. As an alternative route, amidases have emerged as promising biocatalysts for hydroxamic acid synthesis through their acyltransferase activity. Recent advancements in the synthesis approaches for hydroxamic acids are reviewed. The biocatalytic routes are explored, emphasizing the use of amidases and their acyltransferase activity. The scope and potential applications of this chemoenzymatic approach in synthesizing various hydroxamic acids and their derivatives are discussed. Such advancements have the potential to revolutionize the production of these important compounds, making the synthesis process more sustainable, efficient, and economically viable.

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“…However, downstream purification accounts for 50–80% of the entire production cost [ 2 ]. There is a severe bottleneck for cost-effective purification of biopharmaceuticals due to the dramatic increase in the number of products and the increased product titer and purity requirements [ 3 ]. Significant efforts have been made in recent years to overcome these challenges in order to improve the efficacy of purification during downstream processing.…”

Section: Introductionmentioning

confidence: 99%

Electrospun Hydrophobic Interaction Chromatography (HIC) Membranes for Protein Purification

2022

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Responsive membranes for hydrophobic interaction chromatography have been fabricated by functionalizing poly(N-vinylcaprolactam) (PVCL) ligands on the substrate of electrospun regenerated cellulose nanofibers. Both static and dynamic binding capacities and product recovery were investigated using bovine serum albumin (BSA) and Immunoglobulin G (IgG) as model proteins. The effects of ligand chain length and chain density on static binding capacity were also studied. A static binding capacity of ~25 mg/mL of membrane volume (MV) can be achieved in optimal ligand grafting conditions. For dynamic binding studies, protein binding capacity increased with protein concentration from 0.1 to 1.0 g/L. Dynamic binding capacity increased from ~8 mg/mL MV at 0.1 g/L BSA to over 30 mg/mL at 1.0 g/L BSA. However, BSA recovery decreased as protein concentration increased from ~98% at 0.1 g/L BSA to 51% at 1 g/L BSA loading concentration. There is a clear trade-off between binding capacity and recovery rate. The electrospun substrate with thicker fibers and more open pore structures is superior to thinner fibrous membrane substrates.

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Bioprocess Scale-up for Acetohydroxamic Acid Production by Hyperactive Acyltransferase of Immobilized Rhodococcus Pyridinivorans

Cited by 13 publications

References 31 publications

Hierarchical Emulsion-Templated Monoliths (polyHIPEs) as Scaffolds for Covalent Immobilization of P. acidilactici

Hierarchical Emulsion-Templated Monoliths (polyHIPEs) as Scaffolds for Covalent Immobilization of P. acidilactici

Enzymatic Catalysts for Hydroxamic Acid Formation: A Mini‐Review

Electrospun Hydrophobic Interaction Chromatography (HIC) Membranes for Protein Purification

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