Bioprospecting culturable and unculturable microbial consortia through metagenomics for bioremediation

Wani, Atif Khurshid; Akhtar, Nahid; Naqash, Nafiaah; Chopra, Chirag; Singh, Reena; Kumar, Vineet; Kumar, Sunil; Mulla, Sikandar I.; Américo-Pinheiro, Juliana Heloísa Pinê

doi:10.1016/j.clce.2022.100017

Cited by 23 publications

(6 citation statements)

References 222 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Extreme environments have shaped biodiversity driven by harsh physicochemical conditions displayed in a myriad of adaptations in microorganisms of high biotechnological value, including microbial enzymes for a plethora of applications ( Manni & Filali-Maltouf, 2022 ; Pascoal, Magalhaes & Costa, 2020 ; Sysoev et al, 2021 ; Tatta et al, 2022 ; Uma et al, 2020 ; Wani et al, 2022a ). Bacterial enzymes possess key features more advantageous than fungal enzymes such as higher growth rates, genetic versatility ( Menendez, Garcia-Fraile & Rivas, 2015 ) and more specific, efficient catalytic activity in harsh conditions; moreover, bacteria produce multienzyme complexes with greater functionality and specificity in broader conditions ( Balla et al, 2022 ; Castiglia et al, 2016 ; Maki, Leung & Qin, 2009 ).…”

Section: Introductionmentioning

confidence: 99%

“…Despite accumulating evidence of catalytic novelty in non-culturable independent studies ( Georgiadou et al., 2021 ; Manni & Filali-Maltouf, 2022 ; Schaller et al., 2022 ; Sysoev et al., 2021 ; Wani et al., 2022a ; Wani et al., 2022b ), and reports from isolated highly valuable microbial collections from unexplored sites ( Gonzalez-Salazar et al., 2023 ; Swiecimska, Golinska & Goodfellow, 2022 ), there is still a need for efficient and detailed systematic bioprospecting studies for cellulolytic actinomycetes. Work describing scrutinization criteria and the rationale behind the selective methodology of microbial collections could enhance bioprospection efforts in underexplored biomes that will effectively filter “gifted microbes” harboring more valuable enzymes and other natural products.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Systematic bioprospection for cellulolytic actinomycetes in the Chihuahuan Desert: isolation and enzymatic profiling

Escudero-Agudelo,

Martínez-Villalobos,

Arocha-Garza

et al. 2023

PeerJ

View full text Add to dashboard Cite

The quest for microbial cellulases has intensified as a response to global challenges in biofuel production. The efficient deconstruction of lignocellulosic biomass holds promise for generating valuable products in various industries such as food, textile, and detergents. This article presents a systematic bioprospection aimed at isolating actinomycetes with exceptional cellulose deconstruction capabilities. Our methodology explored the biodiverse oligotrophic region of Cuatro Cienegas, Coahuila, within the Chihuahuan Desert. Among the evaluated actinomycetes collection, 78% exhibited cellulolytic activity. Through a meticulous screening process based on enzymatic index evaluation, we identified a highly cellulolytic Streptomyces strain for further investigation. Submerged fermentation of this strain revealed an endoglucanase enzymatic activity of 149 U/mg. Genomic analysis of strain Streptomyces sp. STCH565-A revealed unique configurations of carbohydrate-active enzyme (CAZyme) genes, underscoring its potential for lignocellulosic bioconversion applications. These findings not only highlight the significance of the Chihuahuan Desert as a rich source of cellulolytic microorganisms but also offer insights into the systematic exploration and selection of high-performing cellulolytic microorganisms for application in diverse environmental contexts. In conclusion, our bioprospecting study lays a foundation for harnessing the cellulolytic potential of actinomycetes from the Chihuahuan Desert, with implications for advancing cellulose deconstruction processes in various industries. The findings can serve as a blueprint for future bioprospecting efforts in different regions, facilitating the targeted discovery of microorganisms with exceptional cellulosic deconstruction capabilities.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Systematic bioprospection for cellulolytic actinomycetes in the Chihuahuan Desert: isolation and enzymatic profiling

Escudero-Agudelo,

Martínez-Villalobos,

Arocha-Garza

et al. 2023

PeerJ

View full text Add to dashboard Cite

show abstract

“…Recent reports illustrate that industries produce millions of tonnes of wastes every year, and this waste generation is anticipated to increase by three times by 2100 (Krishnan et al, 2021). Consequent amassment of environmental contaminants from industrial wastes in marine, freshwater, and terrestrial ecosystems has been a matter of serious concern (Tornero and Hanke, 2016;Wani et al, 2022). This has concomitantly aggravated the environmental problems related to industrial waste 2 management and disposal (Sandberg et al, 2019).…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

Role of bulking agents, process optimization, and different earthworm species in the vermiremediation process of industrial wastes: A review

2023

View full text Add to dashboard Cite

Rapid industrialization and consumerism have aggravated the generation of industrial waste globally, consequently posing a serious problem related to their treatment, disposal, and management. Industrial wastes or sludges are mainly characterised by undesirable levels of heavy metals, toxic chemicals, and other toxic organic compounds. Deposition of such wastes in the environmental matrices for prolonged periods may result in serious contamination, and the consequent accumulation of these harmful constituents in the ecological food chain. Unavailability of appropriate disposal mechanisms for these sludges is a matter of serious concern that could severely pollute the environment and risk human health. Vermicomposting has emerged as a feasible and environmentally friendly bioremediation technology that could provide a solution to this problem. However, the vermicomposting of industrial sludges requires a better understanding of its inextricable factors to make it a viable process. Thus, the present study was undertaken to provide insights on the influence of different bulking agents and abiotic factors on the vermicomposting process, as well as, the role of different earthworm species in the successful implementation of this process in the bioremediation of industrial waste.

show abstract

“…Bioremediation strategies using microorganisms are defined by their indigenous diverse consortia and metabolic potential. Isolation and purification of such diverse consortia of organisms offers certain acquaintance regarding the degradation process and microbial metabolites (Wani et al, 2022) (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Marine bacteria and omic approaches: A novel and potential repository for bioremediation assessment

Hassan

Sabreena

Khurshid

et al. 2022

Journal of Applied Microbiology

Self Cite

View full text Add to dashboard Cite

Marine environments accommodating diverse assortments of life constitute a great pool of differentiated natural resources. The cumulative need to remedy unpropitious effects of anthropogenic activities on estuaries and coastal marine ecosystems has propelled the development of effective bioremediation strategies. Marine bacteria producing biosurfactants are promising agents for bio‐remediating oil pollution in marine environments, making them prospective candidates for enhancing oil recovery. Molecular omics technologies are considered an emerging field of research in ecological and diversity assessment owing to their utility in environmental surveillance and bioremediation of polluted sites. A thorough literature review was undertaken to understand the applicability of different omic techniques used for bioremediation assessment using marine bacteria. This review further establishes that for bioremediation of environmental pollutants (i.e. heavy metals, hydrocarbons, xenobiotic and numerous recalcitrant compounds), organisms isolated from marine environments can be better used for their removal. The literature survey shows that omics approaches can provide exemplary knowledge about microbial communities and their role in the bioremediation of environmental pollutants. This review centres on applications of marine bacteria in enhanced bioremediation, using the omics approaches that can be a vital biological contrivance in environmental monitoring to tackle environmental degradation. The paper aims to identify the gaps in investigations involving marine bacteria to help researchers, ecologists and decision‐makers to develop a holistic understanding regarding their utility in bioremediation assessment.

show abstract

Bioprospecting culturable and unculturable microbial consortia through metagenomics for bioremediation

Cited by 23 publications

References 222 publications

Systematic bioprospection for cellulolytic actinomycetes in the Chihuahuan Desert: isolation and enzymatic profiling

Systematic bioprospection for cellulolytic actinomycetes in the Chihuahuan Desert: isolation and enzymatic profiling

Role of bulking agents, process optimization, and different earthworm species in the vermiremediation process of industrial wastes: A review

Marine bacteria and omic approaches: A novel and potential repository for bioremediation assessment

Contact Info

Product

Resources

About