Bioprospecting in potato fields in the Central Andean Highlands: Screening of rhizobacteria for plant growth-promoting properties

Ghyselinck, Jonas; Velivelli, Siva L. S.; Heylen, Kim; O’Herlihy, Eileen; Franco, Javier; Rojas, Mercy; Vos, Paul De; Prestwich, Barbara Doyle

doi:10.1016/j.syapm.2012.11.007

Cited by 75 publications

(54 citation statements)

References 65 publications

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“…30 In a field trial conducted in Bolivia, a reduced level of R. solani infection was observed on potato tuber plants that had been treated with Pseudomonas sp. R41805 (Unpublished data).…”

Section: Resultsmentioning

confidence: 99%

“…Most studies tend to use model plants instead of crops to comprehensively analyze the interactions between plants and microorganisms and the knowledge derived, contributes to formulating new strategies to engineer broad-spectrum resistance, underpinned by rational biotechnological approaches. The efficiency of defense bioprimers can be influenced by environmental factors, while plant-inoculant interactions can largely be determined by genotype; 30 consequently, it is necessary to conduct trials that assess both host and environmental compatibility to confirm the efficiency of inoculants with different plant genotypes. Establishing the priming efficiency for agriculture, requires further research, but the promise of the use of bioprimers for crop plant protection, may intensify the search for soil microorganisms that can improve plant resistance and minimise pathogen-induced production losses.…”

Section: E988076-4mentioning

confidence: 99%

“…30 Bacterial stock cultures were maintained in tryptic soy broth (TSB) supplemented with 70% glycerol at ¡40 C. For routine use, the rhizobacterial strain was streaked onto tryptic soy agar (TSA).…”

Section: Biological Materialsmentioning

confidence: 99%

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The induction of Ethylene response factor 3 (ERF3) in potato as a result of co-inoculation withPseudomonassp. R41805 andRhizophagus irregularisMUCL 41833 – a possible role in plant defense

Velivelli

Lojan

Cranenbrouck

et al. 2015

Plant Signaling & Behavior

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“…30 In a field trial conducted in Bolivia, a reduced level of R. solani infection was observed on potato tuber plants that had been treated with Pseudomonas sp. R41805 (Unpublished data).…”

Section: Resultsmentioning

confidence: 99%

Section: E988076-4mentioning

confidence: 99%

See 1 more Smart Citation

The induction of Ethylene response factor 3 (ERF3) in potato as a result of co-inoculation withPseudomonassp. R41805 andRhizophagus irregularisMUCL 41833 – a possible role in plant defense

Velivelli

Lojan

Cranenbrouck

et al. 2015

Plant Signaling & Behavior

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“…Numerous positive traits have been attributed to Pseudomonas strains, ranging from denitrification (Zhang et al, 2011) (e.g. in wastewater treatments) to the degradation of toxic components (Farhadian et al, 2008;Khan et al, 2009) and the promotion of plant growth (Chin-A-Woeng et al, 2003;Daayf et al, 2003;De Curtis et al, 2010;Ghyselinck et al, 2013;Haas & Défago, 2005;Kim & Jeun, 2006;Scherwinski et al, 2008;Yan et al, 2002). However, the genus Pseudomonas also has a negative reputation, as it includes opportunistic human pathogens (Gershman et al, 2008) and plant pathogens (Mansfield et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

An rpoD gene sequence based evaluation of cultured Pseudomonas diversity on different growth media

et al. 2013

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The last decade has shown an increased interest in the utilization of bacteria for applications ranging from bioremediation to wastewater purification and promotion of plant growth. In order to extend the current number of micro-organism mediated applications, a continued quest for new agents is required. This study focused on the genus Pseudomonas, which is known to harbour strains with a very diverse set of interesting properties. The aim was to identify growth media that allow retrieval of a high Pseudomonas diversity, as such increasing the chance of isolating isolates with beneficial properties. Three cultivation media: trypticase soy agar (TSA), potato dextrose agar (PDA) and Pseudomonas isolation agar (PIA) were evaluated for their abilities to grow Pseudomonas strains. TSA and PDA were found to generate the largest Pseudomonas diversity. However, communities obtained with both media overlapped. Communities obtained with PIA, on the other hand, were unique. This indicated that the largest diversity is obtained by sampling from either PDA or TSA and from PIA in parallel. To evaluate biodiversity of the isolated Pseudomonas members on the media, an appropriate biomarker had to be identified. Hence, an introductory investigation of the taxonomic resolution of the 16S rRNA, rpoD, gyrB and rpoB genes was performed. The rpoD gene sequences not only had a high phylogenetic content and the highest taxonomic resolution amongst the genes investigated, it also had a gene phylogeny that related well with that of the 16S rRNA gene.

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“…Ages of mutualism between potato plants and soil bacteria in this region support the hypothesis that Andean soils harbor interesting plant growthpromoting bacteria (GHYSELINCK et al 2013). In this context, systemic acquired resistance has a very interesting practical aspect.…”

Section: Introductionmentioning

confidence: 53%

Desempenho agronômico da batata (Solanum tuberosum L.) cv. ´Unica´ sob inoculação com rhizobacterias nativas e aplicação de ácido acetil salicílico

Contreras-Liza

Sanchez

Dávila³

2018

Rev. Ciên. Agrovet.

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Peru is center of origin of potato and its wild relatives, so it is convenient to develop new technologies of agronomical management with less impact on the ecosystem, as in the use of pesticides and chemical fertilizers in potato is increasing in developing countries. The objective of the work was to evaluate the agronomic performance of potato cv. ´Unica´ under inoculation with PGPR rhizobacteria isolated from the rhizosphere of Andean potatoes and on the effect of the application of acetylsalicylic acid in field conditions of the Peruvian coast. Inoculation of four bacterial isolates were evaluated: Bacillus simplex B13, Bacillus amyloliquefaciens Bac 15Mb, Azotobacter sp. Azo 16M2 and Pantoea sp. DZ22, as well as a dose of 0.4 mM acetyl salicylic acid as promoter treatments for plant growth versus a control with no inoculation. A randomized complete block design was used in a sandy loam class soil, slightly alkaline, with low organic content and free of salts. There was a relative humidity between 54 and 71%, while the average air temperatures ranged between 13 and 21 °C in San Vicente de Cañete, Lima (coordinates UTM 18L3540788550372). Inoculated plants with bacterial strains at sowing time and control were evaluated at flowering in plots of 40 plants with three replications for several physiological and productive parameters. Means were separated statistically through Duncan's multiple range test and processed with Infostat software. Inoculation with strain Azotobacter sp. Azo 16M2, showed significant differences (p<0.05) with respect to control without inoculation, increasing tuber yield and commercial tuber weight per hectare and with less damage by the leafminer fly (Liriomyza huidobrensis) vis-à-vis the control. Also application of acetyl salicylic acid showed higher percentage of inflorescences (p<0.05) compared to the control, while statistical differences were not found between bacterial strains for this character. It was concluded that the application of inoculant strain Azotobacter sp. Azo 16M2 improved the agronomic performance of potato cv. 'Unica', under subtropical conditions of the valley of Cañete in terms of production of tubers and commercial quality, and less foliage damage by the leafminer fly compared to non-inoculated plants. KEYWORDS:Azotobacter, agroecology, PGPR, potato-associated bacteria, plant-microbe interaction, salicylic acid. RESUMOPeru é o centro de origem da batata e seus parentes silvestres, por isso é conveniente desenvolver novas tecnologias de gestão agronômica com menor impacto no ecossistema, como o uso de pesticidas e fertilizantes químicos em batata está aumentando nos países em desenvolvimento. O objetivo do trabalho foi avaliar o desempenho agronômico da cv. ´Unica´ sob inoculação com rizobactérias PGPR isoladas da rizosfera de batata andina e pelo efeito da aplicação de ácido acetilsalicílico nas condições de campo da costa peruana. Avaliou-se a inoculação de quatro isolados bacterianos: Bacillus simplex B13, B. amyloliquefaciens Bac 15Mb, Azotobacter sp....

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Bioprospecting in potato fields in the Central Andean Highlands: Screening of rhizobacteria for plant growth-promoting properties

Cited by 75 publications

References 65 publications

The induction of Ethylene response factor 3 (ERF3) in potato as a result of co-inoculation withPseudomonassp. R41805 andRhizophagus irregularisMUCL 41833 – a possible role in plant defense

The induction of Ethylene response factor 3 (ERF3) in potato as a result of co-inoculation withPseudomonassp. R41805 andRhizophagus irregularisMUCL 41833 – a possible role in plant defense

An rpoD gene sequence based evaluation of cultured Pseudomonas diversity on different growth media

Desempenho agronômico da batata (Solanum tuberosum L.) cv. ´Unica´ sob inoculação com rhizobacterias nativas e aplicação de ácido acetil salicílico

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