Bioreactor Synergy with 3D Scaffolds: New Era for Stem Cells Culture

Yi, Tianqi; Huang, Shaoxiong; Liu, Guiting; Li, Tiancheng; Kang, Yang; Luo, Yuxi; Wu, Jun

doi:10.1021/acsabm.8b00057

Cited by 27 publications

(15 citation statements)

References 161 publications

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“…The synergy of 3D scaffolds and perfusion bioreactors represents a promising strategy to more accurately simulate growth environment of stem cells in the human body [ 34 ]. In bone tissue engineering, 3D scaffolds must be designed to provide a more conductive structure for hMSC attachment, growth and osteogenic differentiation [ 34 ]. Herein, bioinspired scaffolds mimicking the osteogenic niche of cancellous bone have been designed through biomimetic mineralization of recombinant collagen and freeze-drying.…”

Section: Discussionmentioning

confidence: 99%

Biomimetic Mineralization Promotes Viability and Differentiation of Human Mesenchymal Stem Cells in a Perfusion Bioreactor

Ramírez-Rodríguez

Pereira

Herrmann

et al. 2021

IJMS

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In bone tissue engineering, the design of 3D systems capable of recreating composition, architecture and micromechanical environment of the native extracellular matrix (ECM) is still a challenge. While perfusion bioreactors have been proposed as potential tool to apply biomechanical stimuli, its use has been limited to a low number of biomaterials. In this work, we propose the culture of human mesenchymal stem cells (hMSC) in biomimetic mineralized recombinant collagen scaffolds with a perfusion bioreactor to simultaneously provide biochemical and biophysical cues guiding stem cell fate. The scaffolds were fabricated by mineralization of recombinant collagen in the presence of magnesium (RCP.MgAp). The organic matrix was homogeneously mineralized with apatite nanocrystals, similar in composition to those found in bone. X-Ray microtomography images revealed isotropic porous structure with optimum porosity for cell ingrowth. In fact, an optimal cell repopulation through the entire scaffolds was obtained after 1 day of dynamic seeding in the bioreactor. Remarkably, RCP.MgAp scaffolds exhibited higher cell viability and a clear trend of up-regulation of osteogenic genes than control (non-mineralized) scaffolds. Results demonstrate the potential of the combination of biomimetic mineralization of recombinant collagen in presence of magnesium and dynamic culture of hMSC as a promising strategy to closely mimic bone ECM.

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Section: Discussionmentioning

confidence: 99%

Biomimetic Mineralization Promotes Viability and Differentiation of Human Mesenchymal Stem Cells in a Perfusion Bioreactor

Ramírez-Rodríguez

Pereira

Herrmann

et al. 2021

IJMS

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“…88−90 MSCs were found in the bone marrow at first and can also be found in other places by specific signals. 91 MSCs have a thin and long morphology as seen from a microscope. Bioprinting is a potent tool for the fabrication of organs that resemble natural tissue through using the directed assembly of building blocks for constructing organs, as well as repair and restoration of tissue by transplantation or injection of cells into impaired or defective regions.…”

Section: Stem-cell-derived Tissuesmentioning

confidence: 99%

Review of Bioprinting in Regenerative Medicine: Naturally Derived Bioinks and Stem Cells

Moghaddam

Khonakdar

Arjmand

et al. 2021

ACS Appl. Bio Mater.

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Regenerative medicine offers the potential to repair or substitute defective tissues by constructing active tissues to address the scarcity and demands for transplantation. The method of forming 3D constructs made up of biomaterials, cells, and biomolecules is called bioprinting. Bioprinting of stem cells provides the ability to reliably recreate tissues, organs, and microenvironments to be used in regenerative medicine. 3D bioprinting is a technique that uses several biomaterials and cells to tailor a structure with clinically relevant geometries and sizes. This technique's promise is demonstrated by 3D bioprinted tissues, including skin, bone, cartilage, and cardiovascular, corneal, hepatic, and adipose tissues. Several bioprinting methods have been combined with stem cells to effectively produce tissue models, including adult stem cells, embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and differentiation techniques. In this review, technological challenges of printed stem cells using prevalent naturally derived bioinks (e.g., carbohydrate polymers and protein-based polymers, peptides, and decellularized extracellular matrix), recent advancements, leading companies, and clinical trials in the field of 3D bioprinting are delineated.

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“…The discrepancies between MSCs and CA—MSCs provoked interest and proved the CA-MSCs’ distinct properties [ 78 ]. The proliferation and diversity of cells are modulated by elements such as pH, dissolved gas, and shear stress [ 79 , 80 ].…”

Section: Mesenchymal Stem Cells Influence In Btementioning

confidence: 99%

Bone Regeneration and Oxidative Stress: An Updated Overview

et al. 2022

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Bone tissue engineering is a complex domain that requires further investigation and benefits from data obtained over past decades. The models are increasing in complexity as they reveal new data from co-culturing and microfluidics applications. The in vitro models now focus on the 3D medium co-culturing of osteoblasts, osteoclasts, and osteocytes utilizing collagen for separation; this type of research allows for controlled medium and in-depth data analysis. Oxidative stress takes a toll on the domain, being beneficial as well as destructive. Reactive oxygen species (ROS) are molecules that influence the differentiation of osteoclasts, but over time their increasing presence can affect patients and aid the appearance of diseases such as osteoporosis. Oxidative stress can be limited by using antioxidants such as vitamin K and N‑acetyl cysteine (NAC). Scaffolds and biocompatible coatings such as hydroxyapatite and bioactive glass are required to isolate the implant, protect the zone from the metallic, ionic exchange, and enhance the bone regeneration by mimicking the composition and structure of the body, thus enhancing cell proliferation. The materials can be further functionalized with growth factors that create a better response and higher chances of success for clinical use. This review highlights the vast majority of newly obtained information regarding bone tissue engineering, such as new co-culturing models, implant coatings, scaffolds, biomolecules, and the techniques utilized to obtain them.

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Bioreactor Synergy with 3D Scaffolds: New Era for Stem Cells Culture

Cited by 27 publications

References 161 publications

Biomimetic Mineralization Promotes Viability and Differentiation of Human Mesenchymal Stem Cells in a Perfusion Bioreactor

Biomimetic Mineralization Promotes Viability and Differentiation of Human Mesenchymal Stem Cells in a Perfusion Bioreactor

Review of Bioprinting in Regenerative Medicine: Naturally Derived Bioinks and Stem Cells

Bone Regeneration and Oxidative Stress: An Updated Overview

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