Bioreactors in Tissue Engineering

Obregón, Raquel; Ramón‐Azcón, Javier; Ahadian, Samad

doi:10.1002/9783527689934.ch6

Cited by 5 publications

(2 citation statements)

References 335 publications

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“…The usage of bioreactors confers an advantage of culturing the in vitro models in a more realistic and controlled environment than a simple in vitro conventional culture. This advantage has arisen the prevalence of bioreactor usage and a large variety of bioreactor systems have been developed for addressing a particular cell and tissue phenotype [2].…”

Section: Introductionmentioning

confidence: 99%

Designing of an Advanced Compression Bioreactor with an Implementation of a Low-Cost Controlling System Connected to a Mobile Application

et al. 2021

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(1) Background: Bioreactors mimic the natural environment of cells and tissues by providing a controlled micro-environment. However, their design is often expensive and complex. Herein, we have introduced the development of a low-cost compression bioreactor which enables the application of different mechanical stimulation regimes to in vitro tissue models and provides the information of applied stress and strain in real-time. (2) Methods: The compression bioreactor is designed using a mini-computer called Raspberry Pi, which is programmed to apply compressive deformation at various strains and frequencies, as well as to measure the force applied to the tissue constructs. Besides this, we have developed a mobile application connected to the bioreactor software to monitor, command, and control experiments via mobile devices. (3) Results: Cell viability results indicate that the newly designed compression bioreactor supports cell cultivation in a sterile environment without any contamination. The developed bioreactor software plots the experimental data of dynamic mechanical loading in a long-term manner, as well as stores them for further data processing. Following in vitro uniaxial compression conditioning of 3D in vitro cartilage models, chondrocyte cell migration was altered positively compared to static cultures. (4) Conclusion: The developed compression bioreactor can support the in vitro tissue model cultivation and monitor the experimental information with a low-cost controlling system and via mobile application. The highly customizable mold inside the cultivation chamber is a significant approach to solve the limited customization capability of the traditional bioreactors. Most importantly, the compression bioreactor prevents operator- and system-dependent variability between experiments by enabling a dynamic culture in a large volume for multiple numbers of in vitro tissue constructs.

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Section: Introductionmentioning

confidence: 99%

Designing of an Advanced Compression Bioreactor with an Implementation of a Low-Cost Controlling System Connected to a Mobile Application

et al. 2021

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“…Cryogels have played significant role in bioreactor systems, a noteworthy focus of tissue engineering, for the expansion and modification of various cells, as well as the production of signaling molecules (Hixon et al, 2017;Obregón et al, 2016;Martin et al, 2004). Specifically, cryogels have shown promise as vehicles for both the storage and transportation of cells (Vrana et al, 2012;Katsen-Globa et al, 2014;Borg et al, 2016).…”

Section: Bioreactormentioning

confidence: 99%

Three‐dimensional cryogels for biomedical applications

Razavi

Qiao

Thakor

2019

J Biomedical Materials Res

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Cryogels are a subset of hydrogels synthesized under sub‐zero temperatures: initially solvents undergo active freezing, which causes crystal formation, which is then followed by active melting to create interconnected supermacropores. Cryogels possess several attributes suited for their use as bioscaffolds, including physical resilience, bio‐adaptability, and a macroporous architecture. Furthermore, their structure facilitates cellular migration, tissue‐ingrowth, and diffusion of solutes, including nano‐ and micro‐particle trafficking, into its supermacropores. Currently, subsets of cryogels made from both natural biopolymers such as gelatin, collagen, laminin, chitosan, silk fibroin, and agarose and/or synthetic biopolymers such as hydroxyethyl methacrylate, poly‐vinyl alcohol, and poly(ethylene glycol) have been employed as 3D bioscaffolds. These cryogels have been used for different applications such as cartilage, bone, muscle, nerve, cardiovascular, and lung regeneration. Cryogels have also been used in wound healing, stem cell therapy, and diabetes cellular therapy. In this review, we summarize the synthesis protocol and properties of cryogels, evaluation techniques as well as current in vitro and in vivo cryogel applications. A discussion of the potential benefit of cryogels for future research and their application are also presented.

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