Biorecognition Elements in Biosensors

Mujica, Michael López; Tamborelli, Alejandro; Vaschetti, Virginia M.; Gallay, Pablo; Perrachione, Fabrizio; Reartes, Daiana F.; Delpino, Rocío; Rodríguez, Marcela D.; Rubianes, María D.; Dalmasso, Pablo R.; Rivas, Gustavo A.

doi:10.1201/9781003189435-8

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“…In the past few years, LSPR-based transducers have been exploited for the development of biosensors [17][18][19], but although powerful, they lack inherent selectivity toward specific analytes [20,21]. Biorecognition elements such as antibodies, enzymes, and oligonucleotides are employed to impart selectivity to biosensors [22,23]. The use of thiolated (R-SH) molecules is the preferred method for bonding biorecognition elements to noble metal NP surfaces [24,25].…”

Section: Introductionmentioning

confidence: 99%

Critical Issues on the Surface Functionalization of Plasmonic Au-Ag/TiO2 Thin Films with Thiolated Oligonucleotide-Based Biorecognition Elements

Costa,

Pereira-Silva,

Sousa

et al. 2024

Biosensors

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This work reports on the surface functionalization of a nanomaterial supporting localized surface plasmon resonances (LSPRs) with (synthetic) thiolated oligonucleotide-based biorecognition elements, envisaging the development of selective LSPR-based DNA biosensors. The LSPR thin-film transducers are composed of noble metal nanoparticles (NPs) embedded in a TiO2 dielectric matrix, produced cost-effectively and sustainably by magnetron sputtering. The study focused on the immobilization kinetics of thiolated oligonucleotide probes as biorecognition elements, followed by the evaluation of hybridization events with the target probe. The interaction between the thiolated oligonucleotide probe and the transducer’s surface was assessed by monitoring the LSPR signal with successive additions of probe solution through a microfluidic device. The device was specifically designed and fabricated for this work and adapted to a high-resolution LSPR spectroscopy system with portable characteristics. Benefiting from the synergetic characteristics of Ag and Au in the form of bimetallic nanoparticles, the Au-Ag/TiO2 thin film proved to be more sensitive to thiolated oligonucleotide binding events. Despite the successful surface functionalization with the biorecognition element, the detection of complementary oligonucleotides revealed electrostatic repulsion and steric hindrance, which hindered hybridization with the target oligonucleotide. This study points to an effect that is still poorly described in the literature and affects the design of LSPR biosensors based on nanoplasmonic thin films.

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