Biosensor for deconvolution of individual cell fate in response to ion beam irradiation

Biosensor Cell-Fit-HD4D for correlation of single-cell fate and microscale energy deposition in complex ion beams

“…The tilt between cell layer plane and confocal planes indicates the separate acquisition on two different microscopes, followed by post-acquisition stitching and image registration. Figure and figure caption were taken from ( Niklas et al., 2022 ). …”

“…In this protocol the cell layer was positioned in the middle of a 1 cm wide spread-out Bragg peak (SOBP) of carbon ions at a water equivalent depth of ∼ 3.5 cm. Planned physical dose levels were 1 Gy and 0.5 Gy, respectively resulting in expected carbon ion beam fluences of 8.43 ∗ 10 6 cm -2 and 4.16 ∗ 10 6 cm -2 , respectively ( Niklas et al., 2022 ). Our irradiation plan was similar to ( Dokic et al., 2016 ).…”

“…This protocol specifically outlines the application of the biosensor using the wafer architecture. Detailed information on the mounting architecture can be found in ( Niklas et al., 2022 ). Here, we describe the tracking of A549 proliferation dynamics and DSB dynamics.…”

“…Registration of both image acquisition steps allows precise ion track assignment to cells and correlation with cellular readouts. For complete details on the use and execution of this protocol, please refer to Niklas et al. (2022) .…”

“…For complete details on the use and execution of this protocol, please refer to Niklas et al. (2022) .…”