Biosensor immunoassays for the detection of bisphenol A

Development of Single-Stranded DNA Aptamers for Specific Bisphenol A Detection

“…Further, aptamer #3 exhibited much stronger affinity to BPA than to BPB, which has very similar structure. In contrast, at least one wellcharacterized BPA antibody has been shown to have cross affinity with BPB (Ohkuma et al, 2002;Marchesini et al, 2005). Thus, the aptamers isolated by our study are superior over corresponding antibodies, and can be used for highlyselective detection of BPA.…”

“…In particular, methods such as enzyme-linked immunosorbent assay (Freymuth et al, 1986;Zheng et al, 2008) showed insensitive assay, because BPA antibody has nonspecific binding, especially for similar molecules, such as Bisphenol B (BPB) (Ohkuma et al, 2002) or the analog, 4,4-Bis-(4-hydroxyphenyl) valeric acid (Marchesini et al, 2005).…”

Design of ultrasensitive bisphenol A–aptamer based on platinum nanoparticles loading to polyethyleneimine-functionalized carbon nanotubes

“…However, they are strongly dependent on the quality of the prepared antibody and there have been some reports about nonspecific binding to the high similar structure of BPA derivatives [23][24]. In order to overcome these drawbacks, optical and electrochemical sensors have been constructed for BPA detection based on using aptamers as a specific molecular recognition element [25][26][27].…”

Spreeta-based biosensor assays for endocrine disruptors

“…The CMD matrix of the CM5 chip enhances the hapten immobilization level due to the higher number of available sites for derivatization in its three-dimensional structure and blocks non-specific binding to the gold surface. These biosensor chips were found suitable and robust in previously described applications in which haptens were immobilized (Marchesini et al, 2005.…”