Biosensors for choline, choline esters and inhibitors of choline esterase

Wollenberger, Ulla; Setz, K.; Scheller, Frieder W.; Löffler, U.; Göpel, Wolfgang; Gruss, R.

doi:10.1016/0925-4005(91)80119-5

Cited by 34 publications

(12 citation statements)

References 6 publications

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“…Free [58] or immobilized [59] enzyme can be used. With acetyl-or butyryl-choline as the substrate, bienzyme electrodes coupling a cholinesterase (acetyl-or butyrylcholinesterase) with choline oxidase (ChOx) have been reported, with measurement based on the detection of either oxygen [60,61] or hydrogen peroxide [62,63], this latter being more sensitive.…”

Section: Pesticidesmentioning

confidence: 99%

Chemically Modified Electrodes in Biosensing

Ciucu¹

2014

J Biosens Bioelectron

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The development of chemical sensors has received a great deal of scientific interest in the last decades. Not only the chemical industry may benefit from these sensors but also the food industry, bio-industry, medicine, environmental control because of their capability to give continuously and reversibly a selective and fast response to the presence of a specific compound in a complex mixture of components, without perturbing the system. Biosensors combine the power of analytical detection techniques with the specificity of biological recognition system and therefore they are the most promising devices today about this selectivity. Furthermore, biosensors possess many unique features such as compact size, simplicity of use, one-step reagentless analysis, absence of radioactivity, etc., that make them very attractive alternatives to conventional bioanalytical techniques. The present short review highlights some modern aspects of Chemically Modified Electrodes (CMEs) based on redox enzymes used in amperometric biosensing, a detection method which has already found a large number of applications in health care, food industry and environmental analysis. Some relevant applications of amperometric biosensors based on CMEs to real sample analysis are also presented and some possible future trends highlighted.

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Section: Pesticidesmentioning

confidence: 99%

Chemically Modified Electrodes in Biosensing

Ciucu¹

2014

J Biosens Bioelectron

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“…In the case of electrochemical biosensors, products of these reactions are usually detected with ion-selective electrodes [ 6,11,12], ion-selective field-effect transistors [13][14][15][16][17], and amperometric electrodes [18][19][20]. Until recentlv, conductometric thin-film transducers were not used for fabrication of the enzyme biosensors for pesticides assay.…”

Section: Llvtroductionmentioning

confidence: 99%

Conductometric biosensors based on cholinesterases for sensitive detection of pesticides

Dzydevich¹,

Шульга²,

Солдаткин³

et al. 1994

Electroanalysis

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ABSTUCTDisposable conductometric microbiosensors based on immobilization of acetyl and butyrylcholinesterases (BuChEs) on interdigitated electrodes were fabricated. Enzymes were immobilized by crosslinking with bovine serum albumin and the differential output between the pair of electrodes covered with the enzyme membrane and the pair covered with albumin only was recorded. On the basis of these biosensors, a biochemical assay for pesticides in liquids was developed. Respective detection limits were 5 X lo-" M for diisopropyl fluorophosphate (DFP), lo-' M for trichlorfon, and M for paraoxon-methyl and paraoxon-ethyl for the assavs based on the enzymes tested. The presence of pesticides in the sample in concentration exceeding the detection limits can be detected after only 10 minutes of incubation, whereas, in order to extend the range of the determined pesticide concentration and to improve the accuracy of the assay, it is better to use incubation times longer than 30 minutes. Use of pyridin-2-aldoximide methiodide as reactivating agent allowed one to achieve the complete recovery of the sensor response when it was incubated in lowconcentrated pesticide solutions, e.g., less than M for paraoxon-methyl and during a short time, e.g., 10 to 20 minutes, for M paraoxon-methyl solution.

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“…The current determination for AchE or Ach is based on the determination of the rate of hydrolysis of the substrate. The techniques most commonly used for the determination are spectrophotometry [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22], high-performance liquid chromatography (HPLC), gas chromatography (GC) using a flame ionization detection, GC with mass spectrometry (GC-MS) [23][24][25][26] and amperometric [27][28][29][30][31][32][33][34][35][36] and potentiometric sensors [27,[37][38][39][40][41][42][43][44]. Most of the potentiometric AchE-based Ach sensors involve pH detection.…”

Section: Introductionmentioning

confidence: 99%