Biosensors: Receptor, Binding Protein, and Peptide Sensors

Tertiş, Mihaela; Canciu, Alexandra; Drăgan, Ana-Maria; Hosu, Oana; Szabó, Sándor; Feier, Bogdan; Cristea, Cécilia

doi:10.1016/b978-0-12-822548-6.00151-5

Cited by 3 publications

(3 citation statements)

References 93 publications

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“…According to researchers, the (NBSs) are a form of discontinuity of anthropic influences on the environment, and can ensure a sustainable growth (TERTIS, et al [11]). It is necessary to present the relationship that in a harmonious way should exist between surface waters extremely relevant to the maintenance of biodiversity in all its aspects, mainly in the hydrological cycle of water, and groundwater that also play this important role in the said cycle.…”

Section: Theoretical Referencementioning

confidence: 99%

Groundwater and Anthropic Activities: A Qualitative Study on Treatment Techniques

Oliveira

2024

BJSTR

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The hydrological water cycle comprises constant water movements, which have great influence on the maintenance of vital natural activities. Groundwater in particular constitutes a portion of aquifers, which are considered mobile reservoirs used mainly as a source of supply and human consumption. However, external influences have become largely responsible for the unbalance of the ecosystem, linked to the unbridled human consumption of finite natural resources and the consequent discharge of waste into the receiving bodies without the necessary treatment. The geological water cycle is interrupted when forests are cut down, springs and rivers are extinguished, and together with this, finally, the lack of basic sanitation alters the ecosystem, causing changes of great relevance to the world's population. Groundwater has suffered relevant impacts due to the loss of biodiversity of ecosystems, such as contamination, overuse, lack of current regulatory legislation for use, and the improvement of decontamination techniques. The analysis of the following activities is necessary, mainly due to the anthropic punctual impacts to the ecosystem, corroborating the need for satisfactory techniques. It is noticeable the need for extreme attention to the waste runoff via sanitary sewage to places that have treatment, but the same is not very efficient in retaining this liability, since some developing countries that have not reached about 90% treatment of sanitary sewage, demonstrating the lack of such activities. Thus, the objective of this study is to verify, by means of exploratory and bibliographic research in research bases, the measures that protect the water. Technological measures and sustainable management tools for this purpose will be reviewed, as well as the evolution of groundwater treatment techniques.

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Section: Theoretical Referencementioning

confidence: 99%

Groundwater and Anthropic Activities: A Qualitative Study on Treatment Techniques

Oliveira

2024

BJSTR

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“…14,15 In recent years, biosensors have gained increasing attention because of their easy handling, rapid analysis, high sensitivity, and selectivity toward detection of analytes. 16 Especially, peptides or proteins interacting with cellulose play an important role in biosensorics. For example, peptides have emerged as attractive molecules that can be attached to cellulose or paper substrates for use as a DNA sensor, 17 hard tissue engineering, 18 testing of essential proteins in human serum, 8 or for point of care testing devices.…”

Section: ■ Introductionmentioning

confidence: 99%

“…It contains a linear homopolymer of d -glucose units linked by β-1,4 glycosidic bonds . In general, the hydroxyl groups of cellulose can be functionalized with bio-organic moieties via established synthesis protocols. , Cellulose-based functionalized materials are widely used in various fields of applications such as drug delivery systems, − supporting materials for catalysts, − and sensor devices. , In recent years, biosensors have gained increasing attention because of their easy handling, rapid analysis, high sensitivity, and selectivity toward detection of analytes . Especially, peptides or proteins interacting with cellulose play an important role in biosensorics.…”

Section: Introductionmentioning

confidence: 99%

Peptides as Model Systems for Biofunctionalizations of Cellulose─Synthesis and Structural Characterization by Advanced Solid-State Nuclear Magnetic Resonance Techniques

Limprasart,

Höfler,

Kunzmann

et al. 2023

J. Phys. Chem. C

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The tailored design of bioactive materials based on cellulose or paper is still a challenging task. It requires detailed knowledge of the structure and interaction of the biofunctionalization with the carrier material at the nanoscale. In this work, the small peptide sequence Acetyl-Pro-Ala-Phe-Gly-OH (peptide 1) that can serve as a model for biofunctionalization is synthesized via solid-phase peptide synthesis, purified, and characterized by high-performance liquid chromatography (HPLC) and mass spectrometry (MS). The as-obtained peptide is bound to microcrystalline cellulose (MCC) via a wet chemical approach. Quantification of the peptide on the MCC carrier is performed by replacing l-proline (Pro) in the peptide sequence by 4-fluoro-l-proline (Pro(19F)) (peptide 2) and applying 19F magic angle spinning nuclear magnetic resonance (MAS NMR). Detailed characterization of the model system is provided by using 1H → 13C cross-polarization magic angle spinning (CP MAS NMR) combined with dynamic nuclear polarization (DNP) to enhance sensitivity. Analysis of the binding of the peptide on MCC necessitates the replacement of l-glycine (Gly) in the sequence by 13C-labeled l-glycine (Gly(13C)) (peptide 3). DNP-enhanced 13C–13C correlation experiments carried out with dipolar assisted rotational resonance (DARR) are then used to analyze the proximity between the model peptide and the MCC carrier. The strength of the dipolar coupling is estimated from the DNP-enhanced 1H → 13C CP MAS double-quantum rotational resonance (DQrotres) experiment. The obtained dipolar coupling between the 13CO carbon of peptide 3 and the C6 carbon of the cellulose is equal to a carbon–carbon distance of about two C–O bond lengths, which strongly suggests the binding of significant amounts of the peptide on MCC via an ester bond.

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Multiplexed electrochemical sensing devices for chronic diseases diagnosis and monitoring

Irimes,

Pusta,

Cernat

et al. 2024

TrAC Trends in Analytical Chemistry

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Biosensors: Receptor, Binding Protein, and Peptide Sensors

Cited by 3 publications

References 93 publications

Groundwater and Anthropic Activities: A Qualitative Study on Treatment Techniques

Groundwater and Anthropic Activities: A Qualitative Study on Treatment Techniques

Peptides as Model Systems for Biofunctionalizations of Cellulose─Synthesis and Structural Characterization by Advanced Solid-State Nuclear Magnetic Resonance Techniques

Multiplexed electrochemical sensing devices for chronic diseases diagnosis and monitoring

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