Biospeckle numerical values over spectral image maps of activity

Braga, Roberto A.; Cardoso, Rafael; Bezerra, Pedro Soares; Wouters, Flademir; Sampaio, Gabriela Rodrigues; Varaschin, Mary Suzan

doi:10.1016/j.optcom.2011.10.079

Cited by 31 publications

(19 citation statements)

References 25 publications

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“…Foram extraídas das 128 imagens interferométricas (640 x 486 pixels) do carcinoma, subimagens (64 x 64 pixels) com região composta somente por tecido canceroso e subimagens compostas somente por tecido saudável, totalizando 256 subimagens. As localizações dos tecidos cancerígenos e saudáveis foram baseadas na pesquisa feita por Braga et al (2012), com análise espectral nas imagens interferométricas, em que foram observadas altas frequências em tecidos cancerígenos e baixas frequências em tecidos saudáveis. Na Figura 1, são mostradas as regiões com tecido saudável e tecido canceroso de uma imagem, segundo Braga et al (2012).…”

Section: Methodsunclassified

“…Para atingir esse objetivo, foram extraídas da sequência de 128 imagens (640 x 486 pixels) subimagens (64 x 64 pixels) das regiões cancerígenas e saudáveis. As localizações dos tecidos cancerígenos e saudáveis foram baseadas na pesquisa feita por Braga et al (2012). As subimagens foram processadas utilizando a análise de multirresolução proposta por Mallat (1989) com a função de ondaleta de Daubechies com oito momentos nulos e quatro níveis de resolução.…”

Section: Introductionunclassified

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SAFADI¹

2020

Rev. Bras. Biom.

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Section: Methodsunclassified

Section: Introductionunclassified

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SAFADI¹

2020

Rev. Bras. Biom.

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“…The raw data of a coffee seed 24 and of a cat's cancer tissue 25 were used to evaluate the feasibility of Gaussian distribution of the selected points around an interested area. In the case of the coffee seed, the interested area was the endosperm of the seed, while the interested area in the cat's tissue was the normal tissue surrounding the cancer tissue.…”

Section: Coffee Seed and Cancer Tissuementioning

confidence: 99%

Dynamic laser speckle analyzed considering inhomogeneities in the biological sample

2017

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Dynamic laser speckle phenomenon allows a contactless and nondestructive way to monitor biological changes that are quantified by second-order statistics applied in the images in time using a secondary matrix known as time history of the speckle pattern (THSP). To avoid being time consuming, the traditional way to build the THSP restricts the data to a line or column. Our hypothesis is that the spatial restriction of the information could compromise the results, particularly when undesirable and unexpected optical inhomogeneities occur, such as in cell culture media. It tested a spatial random approach to collect the points to form a THSP. Cells in a culture medium and in drying paint, representing homogeneous samples in different levels, were tested, and a comparison with the traditional method was carried out. An alternative random selection based on a Gaussian distribution around a desired position was also presented. The results showed that the traditional protocol presented higher variation than the outcomes using the random method. The higher the inhomogeneity of the activity map, the higher the efficiency of the proposed method using random points. The Gaussian distribution proved to be useful when there was a well-defined area to monitor.

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“…I will not list all of them here, since the objective is to present breakpoints regarding BSL developments rather than provide a list of research works. Otherwise, we can cite some applications in medicine using offline techniques such as identification of cancer adjacent to normal tissue in animal samples (Braga et al, 2012) or even changes in muscle tissues (Maksymenko;Muravsky;Berezyuk, 2015). Offline methods can also be applied to identify parasites (Pomarico et al, 2004;Grassi et al, 2016) or even the effect of drugs in cancer cells (González-Peña et al, 2014).…”

Section: Analysis Of Biospeckle Laser Applicationsmentioning

confidence: 99%

When noise became information: State-of-the-art in biospeckle laser

Braga

Alves²

2017

Ciênc. agrotec.

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Laser was presented to science and industry in the 1960s and shortly became a useful tool in many areas, with applications based on its multiple characteristics such as coherence of light, which presents a phenomenon known as interference pattern, or speckle, when beam returns from an illuminated surface. Despite great application of speckle pattern, its residual presence, for example, in interferometric approaches was considered as a noise, demanding filtering. However, grains themselves became information as their dynamic changes in time started to be linked to biological sample activity. Dynamic laser speckle has been since then a phenomenon widely used to monitor biological activities in many areas from agriculture to medicine. It is known as biospeckle laser (BSL) when adopted in biological material, with high sensitivity to follow very tiny movements in biological tissues, linked to changes in speckle provided by scatterer activities inside and outside cells. Since the 1970s, biospeckle laser usage follows a crescent technologic spiral where technological developments opened room for new applications, while new demands regarding biological monitoring forced the development of new methodologies. Therefore, potential adoption of the phenomenon as a sensor, for instance, in agricultural and medical processes, as well as constant offer of new devices provided new turns in the BSL technologic spiral and opened room for technique improvement. In this study, I present a short history of biospeckle laser (BSL) with applications and development associated with challenges regarding its usage in portable and accessible devices or even in commercial equipment. And the history was packed in a temporal diagram identifying the breakpoints responsible for improvements in the use of the technique.Index terms: Optical metrology; biosystems; challenges. RESUMOO laser foi apresentado à ciência e à indústria em 1960, e rapidamente tornou-se uma ferramenta útil à muitas áreas do conhecimento, que fazem uso de suas múltiplas características, como é o caso da coerência da luz, que é responsável pelo fenômeno conhecido como padrão de interferência, ou speckle; este padrão de interferência ocorre quando a luz retorna do material iluminado formando uma figura de interferência para o observador. Apesar da grande aplicação, a presença residual do padrão de speckle em abordagens interferométricas apresentavase como um ruído que demanda filtragem. Apesar da dificuldade em alguns casos, os grãos de speckle tornaram-se fonte de informação em situações dinâmicas, quando suas mudanças começaram a ser relacionadas à atividade do material biológico iluminado. Speckle laser dinâmico tornou-se o termo usado para denominar o fenômeno que passou a ser usado para monitorar a atividade biológica e não biológica em aplicações desde a agricultura até aquelas relacionadas à medicina. Este fenômeno também é conhecido como biospeckle laser (BSL), quando aplicado em material biológico, e com grande sensibilidade para seguir os menores movimentos ...

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Biospeckle numerical values over spectral image maps of activity

Cited by 31 publications

References 25 publications

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Dynamic laser speckle analyzed considering inhomogeneities in the biological sample

When noise became information: State-of-the-art in biospeckle laser

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