“…bOCT has been successfully employed to monitor biological activities inside plants and has been demonstrated to investigate the effects of Ozone on leaves [ 36 , 37 ], and growth hormone, gibberellic acid (GA3) [ 38 ]. bOCT has also been used successfully to study seed germination under the impact of environmental conditions [ 39 ] on such as, Zn [ 13 ], PEMPs alone on lentil seeds [ 7 ], Acid Mine Drainage's (AMD) [ 40 , 41 ] and effect of AMD on monocot and dicot seed germination [ 42 ]. In the present study, bOCT was used to assess the individual toxic effect of PEMPs and Zn and their synergic effects followed by comparison with the conventional measures.…”