Biosurfactant-producing Rhodococcus ruber TD2 isolated from oil poluted water in vung tau coastal zone

Abstract

Thanks to their advantages compared with other microbial and synthetic surfactants, Rhodococcus biosurfactants are widely studied to apply in new advanced technologies, such as environmental bioremediation, improved polymeric material construction and biomedicine. A biosurfactant-producing strain TD2 was isolated from the oil poluted water at Vung Tau coastal zone. Strain TD2 was observed as rodshaped, positive gram, non-spore bacterium. The strain was identified as Rhodococcus ruber TD2(100 identity Rhodococcus ruber X 80625) based on 16S rDNA analysis. Investigation of influence of environmental conditions on cell growth and biosurfactant production showed that strain TD2 produced highest biosurfactant at 30 o C, pH 8-9, 1-2% NaCl (w/v) and olive oil as carbon source. In this condition, Rhodococcus ruber TD2 exhibited to be a potential producer attaining 94% of emulsifying index E24 and the maximum yield of crude biosurfactant production was 13.7 g/l. GC-MS analysis revealed that biosurfactant of Rhodococcus ruber TD2 was ester of Hexadecenoic acid or Hexanedioic acid bis 2-ethylhexyl containing of many -OH and C=O groups in structural chemicals.

Identification of hydrocarbon-degrading bacterial consortium isolated from the oil-contaminated muddy soil in Hanoi, Vietnam

“…Mineral salt medium (GOST 9023-74) contained the following: KNO 3 -4 g; KH 2 PO 4 -0.5 g; Na 2 HPO 4 -1.4 g; MgSO 4 -0.8 g, distilled water 1L, addition 5% crude oil mixed in DO (at a ratio of 5:95). The DO fuel used for enrichment and the petroleum products used for biodegradation experiments were collected from petroleum company (Petrolimex, Vietnam) (Hien et al, 2013). For solid medium, GOST 9023-74 broth was supplemented with 20 g/L agar.…”

Biosynthesis of Surfactants by Actinobacteria of Rhodococcus Genus

“…Так, у процесі культивування штаму R. equi P20 на соєвій олії (2,5%) концентрація синтезованих ПАР (фосфоліпопептиди) становила 7,3 г/л [34]. R. ruber TD2, який синтезує ПАР на дизельному паливі [23], утворює 13,7 г/л поверхнево-активних речовин на оливковій олії [24]. У роботі [35] Так, за умов росту штаму Au-1 на рафінованій соняшниковій і оливковій олії концентрація таких ПАР становила 1,73 і 2,9 г/л відповідно, на вазеліновій оливі -3,48, а на фузі олійному -2,62 г/л.…”

Characterization of hydrocarbon degrading bacteria isolated from Indian crude oil reservoir and their influence on biocorrosion of carbon steel API 5LX

“…2c) and octadecanoic acid, methyl ester (C 19 H 38 O 2 ) ( Figure 2d) accounting for 17% of the whole peaks present in the GC spectra. Hexanedioic acid, bis (2-ethylhexyl) ester (C 22 H 42 O 4 ) (Hien et al, 2013) was present in the remaining strains such as B. pumilus B1 ( Fig. S1), B. subtilis B5 ( Fig.…”