Biosurfactant production by Bacillus subtilis using corn steep liquor as culture medium

Abstract

In this work, biosurfactant production by Bacillus subtilis #573 was evaluated using corn steep liquor (CSL) as culture medium. The best results were obtained in a culture medium consisting of 10% (v/v) of CSL, with a biosurfactant production of about 1.3 g/l. To the best of our knowledge, this is the first report describing biosurfactant production by B. subtilis using CSL as culture medium. Subsequently, the effect of different metals (iron, manganese, and magnesium) on biosurfactant production was evaluated using the medium CSL 10%. It was found that for all the metals tested, the biosurfactant production was increased (up to 4.1, 4.4, and 3.5 g/l for iron, manganese, and magnesium, respectively). When the culture medium was supplemented with the optimum concentration of the three metals simultaneously, the biosurfactant production was increased up to 4.8 g/l. Furthermore, the biosurfactant exhibited a good performance in oil recovery assays when compared with chemical surfactants, which suggests its possible application in microbial enhanced oil recovery or bioremediation.

Production and applications of lipopeptide biosurfactant for bioremediation and oil recovery by Bacillus subtilis CN2

“…Vazet al[46]and Ghojavand et al[47] reported surface tensions reductions to 29 mN/m and 27 mN/m respectively.For the B. subtillis CN2, the highest amount of biosurfactant produced was7150 mg L −1 of culture medium obtained when using glycerol as the carbon source at concentration of 6% (w/v). Gudiña et al[48] reported about 1300 mg L −1 of biosurfactant production by Bacillus subtilis #573 strain using corn steep liquor (CSL) culture medium consisting of 10% (v/v) of CSL. Lin et al[49] and Rahman et…”

Screening concepts, characterization and structural analysis of microbial-derived bioactive lipopeptides: a review

“…[73,77,78] However, subsequent studies indicated that LP production was suitable using a number of different complex media as well as industrial wastes. These included King's B medium [50] Landy's medium, [82] corn steep liquor, [83] and glycerol waste from biodiesel production. [27,74] Optimal medium and cultivation conditions should be selected for each individual LP/BS producer to reach maximum productivity, although amounts of LP sufficient for analyses (Figure 1) can also be obtained from non-optimized cultures.…”

Optimization of crude oil degradation by Dietzia cinnamea KA1, capable of biosurfactant production

“…Biosurfactants are surface-active compounds synthesized by a wide range of microorganisms [13]. They are amphiphilic molecules containing hydrophobic and hydrophilic moieties and tend to interact with surfaces of different polarities and reduce the surface and interfacial tensions of solutions [5].…”