Biosurfactant Production Using Mutant Strains of Pseudomonas aeruginosa and Bacillus subtilis from Agro-industrial Wastes

Abstract

Purpose: Biosurfactants are applied in drug formulations to improve drug solubility and in some cases, treat diseases. This study is focused on generating, extracting, purifying and then characterizing biosurfactants from bacterial isolates of palm oil wastes and abattoir soil origins. Methods: Eight (8) bacteria were isolated from the soil and sludge samples, out of which four (50 %) were found to produce biosurfactants. Bacillus subtilis (37.5 %) and Pseudomonas aeruginosa (50%) were isolated and identified from these samples using mineral salt medium, nutrient agar and Cetrimide agar. Mutant isolates of Bacillus subtilis BS3 and Pseudomonas aeruginosa PS2 were used to produce biosurfactants using mineral salt medium as enrichment medium and extraction was done using membrane filter. Results: The mutant strains Bacillus subtilis BS3 and Pseudomonas aeruginosa PS2generated biosurfactants that displayed significant solubility and dissolution properties by enhancing the percentage solubility of piroxicam to 62.86 and 54.29 % respectively, and achieved 51.71 and 48.71 % dissolution of the drug in 0.1N HCl. Conclusion: From the results obtained, the produced biosurfactants could serve as a better alternative to conventional surfactants. Notably, the study indicated that the biosurfactant produced by mutant strain of Bacillus subtilis produced more potent activities (surface tension reduction ability, high emulsification) than those of Pseudomonas aeruginosa. Keywords: Biosurfactant production; Bacillus subtilis; Pseudomonas aeruginosa; Microbial Biotechnology; Palm oil wastes; Abattoir soil

Geochemical and Microbiological Composition of Soils and Tailings Surrounding the Komsomolsk Tailings, Kemerovo Region, Russia

“…Strains with antibiotics are typical for rich soil samples. Pseudomonas sp., which are biosurfactant-producing bacterial strains [46], were also found. Pseudomonas play an important role in As biocirculation.…”

Synergistic effects of Bacillus velezensisSDTB038 and phenamacril on Fusarium crown and root rot of tomato

“…We adopted the method of Favaro et al (2016) as the standard for lipopeptide extraction. The bacteria that had grown on nutrient agar (nutrient agar: 20 g agar per litre of nutrient broth; nutrient broth: 3 g/L beef extract, 10 g/L peptone and 5 g/L NaCl; Adejumo et al, 2021) were transferred to 500 mL nutrient broth for subculturing by shaking in the dark at 28°C for 60 h. To extract the crude lipopeptide extract, the cultured cells were pelleted by centrifugation at 4°C, 1500 g for 20 min. The supernatant was acidified to pH 2 with 6 M hydrochloric acid, resulting in the precipitation of a white precipitate from the supernatant.…”

Prospects of Microbial Bio‐surfactants To Endorse Prolonged Conservation in the Pharmaceutical and Agriculture Industries

“…A significant number of currently available publications have demonstrated their efficacy in biosurfactant production and their role in successfully managing phytopathogens. [5,[117][118][119] Varnier et al, [120] concluded that Pseudomonas aeruginosa produced Rhamnolipid biosurfactants that improved grapevine immunity against Botrytis cinerea. In addition, using surfactants prevents the virus's spores from germinating and slows the growth of the mycelium they produce.…”

“…The bacterial genera Pseudomonas and Bacillus are the ones that are utilized the most frequently in biosurfactant manufacturing. A significant number of currently available publications have demonstrated their efficacy in biosurfactant production and their role in successfully managing phytopathogens [5,117–119] . Varnier et al., [120] concluded that Pseudomonas aeruginosa produced Rhamnolipid biosurfactants that improved grapevine immunity against Botrytis cinerea .…”