Biosurfactants: Production, properties, applications, trends, and general perspectives

Sarubbo, Leonie Asfora; Silva, Maria da Glória C.; Durval, Italo José Batista; Bezerra, Káren Gercyane Oliveira; Ribeiro, Beatriz Galdino; Silva, Ivison A.; Twigg, Matthew S.; Banat, İbrahim M.

doi:10.1016/j.bej.2022.108377

Cited by 214 publications

(151 citation statements)

References 193 publications

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“…Biosurfactants have a wide range of applications in industries, such as food, medical, pharmaceutical, petrochemical, and agriculture. They have been demonstrated to have the ability to significantly decrease the surface tension and interfacial tension and form microemulsions [ 10 ].…”

Section: Resultsmentioning

confidence: 99%

“…The development of recombinant biosurfactants is gaining scientific attention in recent times due to industrial applications. A strategy for improving the gene expression that encodes a biosurfactant is receiving focused interest to improve the yields and modify the chemical attributes [ 10 ]. The mycosubtilin and iturin A operons have been determined as operons of the iturin lipopeptide family.…”

Section: Introductionmentioning

confidence: 99%

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Gene Expression and Characterization of Iturin A Lipopeptide Biosurfactant from Bacillus aryabhattai for Enhanced Oil Recovery

Yaraguppi

Bagewadi

Mahanta

et al. 2022

Gels

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Biosurfactants are eco-friendly surface-active molecules recommended for enhanced oil recovery techniques. In the present study, a potential lipopeptide (biosurfactant) encoding the iturin A gene was synthesized from Bacillus aryabhattai. To improvise the yield of the lipopeptide for specific applications, current research tends toward engineering and expressing recombinant peptides. An iturin A gene sequence was codon-optimized, amplified with gene-specific primers, and ligated into the pET-32A expression vector to achieve high-level protein expression. The plasmid construct was transformed into an E. coli BL21 DE3 host to evaluate the expression. The highly expressed recombinant iturin A lipopeptide was purified on a nickel nitrilotriacetic acid (Ni-NTA) agarose column. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) revealed that the purity and molecular mass of iturin A was 41 kDa. The yield of recombinant iturin A was found to be 60 g/L with a 6.7-fold increase in comparison with our previously published study on the wild strain. The approach of cloning a functional fragment of partial iturin A resulted in the increased production of the lipopeptide. When motor oil was used, recombinant protein iturin A revealed a biosurfactant property with a 74 ± 1.9% emulsification index (E24). Purified recombinant protein iturin A was characterized by mass spectrometry. MALDI-TOF spectra of trypsin digestion (protein/trypsin of 50:1 and 25:1) showed desired digested mass peaks for the protein, further confirming the identity of iturin A. The iturin A structure was elucidated based on distinctive spectral bands in Raman spectra, which revealed the presence of a peptide backbone and lipid. Recombinant iturin A was employed for enhanced oil recovery through a sand-packed column that yielded 61.18 ± 0.85% additional oil. Hence, the novel approach of the high-level expression of iturin A (lipopeptide) as a promising biosurfactant employed for oil recovery from Bacillus aryabhattai is not much reported. Thus, recombinant iturin A demonstrated its promising ability for efficient oil recovery, finding specific applications in petroleum industries.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gene Expression and Characterization of Iturin A Lipopeptide Biosurfactant from Bacillus aryabhattai for Enhanced Oil Recovery

Yaraguppi

Bagewadi

Mahanta

et al. 2022

Gels

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“…In high-performance liquid chromatography (HPLC), the sample is analyzed in the chromatographic column thanks to the mobile phase pumped by plumping system. The detector responds to the elution of the sample, signaling a peak on the chromatogram [73]. Lipopeptide separation is commonly accomplished using HPLC coupled to refractive index, UV, fluorescence, electrochemical, near-infrared, MS, NMR, and light scattering [73,74].…”

Section: Characterization Of Bioemulsifiers By Various Chromatographi...mentioning

confidence: 99%

“…The detector responds to the elution of the sample, signaling a peak on the chromatogram [73]. Lipopeptide separation is commonly accomplished using HPLC coupled to refractive index, UV, fluorescence, electrochemical, near-infrared, MS, NMR, and light scattering [73,74]. The sample is treated with trifluoroacetic acid (TFA) and centrifuged to remove solid particles before being analyzed in an HPLC facility.…”

Section: Characterization Of Bioemulsifiers By Various Chromatographi...mentioning

confidence: 99%

Production and Characterization of a Bioemulsifier Derived from Microorganisms with Potential Application in the Food Industry

Thraeib

Altemimi

Al-Manhel

et al. 2022

Life

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There is a growing interest in the development and use of natural emulsifiers, which provide biodegradability as well as non-toxicity along with giving better performance compared to existing emulsifying agents used in the food industry. A large variety of sources of starting material, i.e., the microorganisms, are available to be used, hence giving a diverse range of applications. The focus of this review paper is on the production of bioemulsifiers, which are said to be “green surfactants”, from fungi, bacteria and yeasts; furthermore, an overview pertaining to the knowledge gained over the years in terms of characterization techniques is reported. The methods used for the characterization and isolation such as TLC, GC-MS, HPLC, NMR have also been studied. The end-application products such as cookies, muffins, and doughs along with the methods used for the incorporation of bioemulsifiers, microorganisms from which they are derived, properties imparted to the product with the use of a particular bioemulsifier and comparison with the existing food grade emulsifiers has been discussed in detail. The future prospects indicate that newer bioemulsifiers with anti-microbial, anti-oxidant and stabilization properties will prove to have a larger impact, and emphasis will be on improving the performance at an economically viable methodology.

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“…This promotes their application, among other things, in crude oil recovery [ 6 , 7 ], in the pharmaceutical industry [ 8 ], or in natural environment bioremediation [ 9 ]. Moreover, they are found in medical [ 10 , 11 , 12 ] and household products [ 10 ]. In numerous practical applications, the surface tension of the aqueous solution of biosurfactants or their mixtures with classical synthetic surfactants plays a very important role.…”

Section: Introductionmentioning

confidence: 99%

Thermodynamic Analysis of the Adsorption and Micellization Activity of the Mixtures of Rhamnolipid and Surfactin with Triton X-165

2022

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The surface tension of aqueous solutions of Triton X-165 with rhamnolipid or surfactin mixtures was measured. The obtained results were applied for the determination of the concentration and composition of the Triton X-165 and biosurfactants mixture at the water–air interface as well as the contribution of the particular component of the mixtures to water surface tension reduction and the mutual influence of these components on the critical micelle concentration. The determination of these quantities was based on both the commonly used concepts and a new one proposed by us, which assumes that the composition of the mixed monolayer at the water–air interface depends directly on the pressure of the monolayer of the single mixture component and allows us to determine the surface concentration of each mixture component independently of surface tension isotherms shape. Taking into account the composition of the mixed monolayer at the water–air interface, the standard Gibbs adsorption free energy was considered. The obtained results allow us to state that the concentration of both mixture components corresponding to their saturated monolayer and the surface tension of their aqueous solution can be predicted using the surfactants’ single monolayer pressure and their mole fraction in the mixed monolayer determined in the proposed way.

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Biosurfactants: Production, properties, applications, trends, and general perspectives

Cited by 214 publications

References 193 publications

Gene Expression and Characterization of Iturin A Lipopeptide Biosurfactant from Bacillus aryabhattai for Enhanced Oil Recovery

Gene Expression and Characterization of Iturin A Lipopeptide Biosurfactant from Bacillus aryabhattai for Enhanced Oil Recovery

Production and Characterization of a Bioemulsifier Derived from Microorganisms with Potential Application in the Food Industry

Thermodynamic Analysis of the Adsorption and Micellization Activity of the Mixtures of Rhamnolipid and Surfactin with Triton X-165

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