Biosynthesis and Characterization, Antioxidant and Antimicrobial Activities of Selenium Nanoparticles from Ethanol Extract of Bee Propolis

Abstract

Aim: Biosynthesis of selenium nanoparticles (SeNPs) has gained significant interest due to their distinctive chemical and biological properties that is essential for potential application in various fields. Methods:In the present study, propolis, the beehive product collected from 5 different Indian states like Haryana, Himachal Pradesh, Uttaranchal, Karnataka and Kerala were used for the biosynthesis of SeNPs and characterized by using UV-vis spectrophotometer, fourier transform spectroscopy (FT-IR), X-ray diffraction (XRD) and scanning electron microscopy (SEM).Results: SeNPs biosynthesized by propolis were observed as crystalline, oval shaped and smooth surface particles. Study also reports the efficiency of 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2,2-diphenyl-1picrylhydrazyl (DPPH), Fluorescence recovery after photobleaching (FRAP) and superoxide dismutase (SOD) assay to estimate the antioxidant potential. The antimicrobial assay was evaluated only for SeNPs synthesized from propolis extracts obtained from Karnataka state showed high antioxidant activity. The antibacterial activity against pathogenic gram-positive bacterial strains (Staphylococcus aureus, Bacillus cereus and Streptococcus mutans), gram-negative bacterial strains (Escherichia coli, Salmonella typhi and Pseudomonas aeruginosa) was evaluated by resazurin microtiter plate method to check the minimum inhibition concentration (MIC). The antifungal activity of pathogenic fungi such as Aspergillus niger, Aspergillus flavon and Candida albicans were determined by well diffusion method. Conclusion:It is the first report describing the biosynthesis of selenium nanoparticles using bee propolis. This study demonstrated the antioxidant and antimicrobial potential of SeNPs biosynthesised by using ethanol extract of propolis.

Synthesis and Structural Characterization of Selenium Nanoparticles–Bacillus sp. MKUST-01 Exopolysaccharide (SeNPs–EPS) Conjugate for Biomedical Applications

“…Therefore, using the wet-chemical approach, in this study, the production of monodisperse SeNPs was achieved by reducing Na 2 SeO 3 with ascorbic acid as a reducing agent and TritonX 100 as the stabilizing agent facilitating the creation of red-colored nanoparticles with an optical property of 265 nm (λmax). This outcome aligns with the findings of Shubharani et al [39], on the synthesis of SeNPs using ethanol extracts of propolis.…”

“…In this study, the XRD pattern suggested that SeNPs is nanocrystalline (Figure 3b) in nature, and it closely resembles the synthesized SeNPs reported in the literature, i.e., as reported during the synthesis of SeNPs with Diospyros montana extract [52] and propolis ethanol extracts [39]. For example, the XRD diffractogram of SeNPs and SeNPs-EPS, as depicted in Figure 3b, displays distinct characteristic diffraction peaks at angles of 23.9°, 30.0°, 41.7°, 44.0°, 45.7°, 52.0°, 56.4°, 62.2°, 65.5°, and 68.4°.…”

“…Figure 2 displays the SeNPs distinctive sharp absorption peak at 268 nm and the intense brick-red colour of the colloidal dispersion. The crystalline nature of SeNPs exhibits absorption maxima at 250 to 280 nm [39,40], and it was similar to our preparations. SeNPs-EPS conjugate shows maximum absorption at 254 nm without a sharp beak (Figure 2), which shows the presence of organic residues (EPS) along with SeNPs.…”

Evaluation the safety and synergistic effect of NiFe2O4 nanoparticles with antibiotic against Pseudomonas aeruginosa

“…It has been reported that as compared to AgNPs alone the combination of antibiotics and AgNPs complexes will release Ag + at a higher rate, moreover it has also been proposed that the combination of antibiotic with AgNPs through the active groups of antibiotics such as hydroxyl group and amine group will result in conjugation of both the molecules. This will result in the increase in the effective concentration of antibiotics at a specific site (27)(28)(29). Pathological study showed clear pathological effect of pseudomonas on infected Rats skin that's agreement with (30).…”

Biosynthesis of selenium nanoparticles from Bacillus species and its applications

“…Nanoparticles, the particles with one or more dimensions of the order of 100nm or less are being used recently in different fields that has dragged the attention of all the scientists in the field of nanotechnology (Fesharaki et al,2009;Singh et al,2014;). The unique properties of nano materials that differ from the bulk metals have considerable application in medicine including cancer therapy, DNA analysis, drug delivery, biosensor and magnetic resonance imaging, apart from agricultural, environmental remediation, pharmaceutical, electronics and commercial industries (Shubharani et al, 2019). Since the beginning of the life, bacteria have been exposed to metal hence the prokaryotes have the ability to resist heavy metal and metalloid toxicity (Llamosas et al, 2016).…”