Biosynthesis and native granule characteristics of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in Delftia acidovorans

Loo, Ching-Yee; Sudesh, Kumar

doi:10.1016/j.ijbiomac.2006.11.003

Cited by 46 publications

(25 citation statements)

References 24 publications

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“…For example, BM 90 can use hydroxybutyric and propionic acid: this is somehow in accord with the work of Loo and Sudesh (2007) that demonstrated the use of these compounds by D. acidovorans for the production of polyhydroxybutyrate and polyhydroxyalcanoate granules.…”

Section: Discussionsupporting

confidence: 64%

Metabolic characterization of a strain (BM90) of Delftia tsuruhatensis showing highly diversified capacity to degrade low molecular weight phenols

et al. 2009

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A novel bacterium, strain BM90, previously isolated from Tyrrhenian Sea, was metabolically characterized testing its ability to use 95 different carbon sources by the Biolog system. The bacterium showed a broad capacity to use fatty-, organic- and amino-acids; on the contrary, its ability to use carbohydrates was extremely scarce. Strain BM90 was identified and affiliated to Delftia tsuruhatensis by molecular techniques based on 16S rRNA gene sequencing. D. tsuruhatensis BM90, cultivated in shaken cultures, was able to grow on various phenolic compounds and to remove them from its cultural broth. The phenols used, chosen for their presence in industrial or agro-industrial effluents, were grouped on the base of their chemical characteristics. These included benzoic acid derivatives, cinnamic acid derivatives, phenolic aldehyde derivatives, acetic acid derivatives and other phenolic compounds such as catechol and p-hydroxyphenylpropionic acid. When all the compounds (24) were gathered in the same medium (total concentration: 500 mg/l), BM90 caused the complete depletion of 18 phenols and the partial removal of two others. Only four phenolic compounds were not removed. Flow cytometry studies were carried out to understand the physiological state of BM90 cells in presence of the above phenols in various conditions. At the concentrations tested, a certain toxic effect was exerted only by the four compounds that were not metabolized by the bacterium.

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Section: Discussionsupporting

confidence: 64%

Metabolic characterization of a strain (BM90) of Delftia tsuruhatensis showing highly diversified capacity to degrade low molecular weight phenols

et al. 2009

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“…The use of propionic and valeric acids as sole carbon sources during fermentation may inhibit the cells growth which is reflected by the low amount of CDW. Both propionic and valeric acids shared a same pKa (4.75e4.87), but propionic acid exhibited more detrimental effect to the cells because it has shorter n-alkyl chains [16]. However, combination of those acids with acetic acid resulted in improvement in CDW, PHA content and composition of 3HV units.…”

Section: Biosynthesis Of Pha During One-step Cultivationmentioning

confidence: 99%

Biosynthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer from wild-type Comamonas sp. EB172

Zakaria

Ariffin

Johar

et al. 2010

Polymer Degradation and Stability

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a b s t r a c tPoly(3-hydroxybutyrate) [P(3HB)] homopolymer and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] copolymer was produced by Comamonas sp. EB172 using single and mixture of carbon sources. Poly(3-hydroxyvalerate) P(3HV) incorporation in the copolymer was obtained when propionic and valeric acid was used as precursors. Incorporation of 3HV fractions in the copolymer varied from 45 to 86 mol% when initial pH of the medium was regulated. In fed-batch cultivation, organic acids derived from anaerobically treated palm oil mill effluent (POME) were shown to be suitable carbon sources for polyhydroxyalkanoate (PHA) production by Comamonas sp. EB172. Number average molecular weight (M n ) produced by the strain was in the range of 153e412 kDa with polydispersity index (M w /M n ) in the range of 2.2e2.6, respectively. Incorporation of higher 3HV units improved the thermal stability of P (3HB-co-3HV) copolymer. Thus the newly isolated bacterium Comamonas sp. EB172 is a suitable candidate for PHA production using POME as renewable and alternative cheap raw materials.Crown

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“…Examples are Cupriavidus necator former Ralstonia eutropha [11][12], Alcaligenes latus [13][14], Comamonas acidovorans ( [14][15][16], Comamonas testosteronii [17] and Hydrogenophaga pseudoflava [18]. Recombinant E. coli has also been used to obtain P(3HB-co-4HB) with 2.8 mol% 4HB [19] as well as to produce the homopolymer P(4HB) [10], for medical purposes at Tepha Inc.…”

Section: Introductionmentioning

confidence: 99%

Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by Burkholderia sacchari using wheat straw hydrolysates and gamma-butyrolactone

Cesário

Raposo

Almeida

et al. 2014

International Journal of Biological Macromolecules

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Burkholderia sacchari DSM 17165 is able to grow and produce poly(3-hydroxybutyrate) both on hexoses and pentoses. In a previous study, wheat straw lignocellulosic hydrolysates (WSH) containing high C6 and C5 sugar concentrations were shown to be excellent carbon sources for P(3HB) production.Using a similar feeding strategy developed for P(3HB) production based on WSH, fedbatch cultures were developed aiming at the production of the copolymer P(3HB-co-4HB) (poly(3-hydroxybutyrate-co-4-hydroxybutyrate)) by B. sacchari. The ability of this * Corresponding author. Tel.: +351 21 8419137; Fax: +351 21 8419062.e-mail address: teresa.cesario@tecnico.ulisboa.pt 2 strain to synthesize P(3HB-co-4HB) was first shown in shake flasks using gammabutyrolactone (GBL) as precursor of the 4HB units.Fed-batch cultures using glucose as carbon source (control) and GBL were developed to achieve high copolymer productivities and 4HB incorporations. The attained P(3HB-co-4HB) productivity and 4HB molar % were 0.7 g/(L·h) and 4.7 molar %, respectively.The 4HB incorporation was improved to 6.3 and 11.8 molar % by addition of 2 g/L propionic and acetic acid, respectively. When WSH were used as carbon source under the same feeding conditions, the values achieved were 0.5 g/(L·h) and 5.0 molar %, respectively. Burkholderia sacchari, a strain able to produce biopolymers based on xylose-rich lignocellulosic hydrolysates, is for the first time reported to produce P(3HB-co-4HB) using gamma butyrolactone as precursor.

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Biosynthesis and native granule characteristics of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in Delftia acidovorans

Cited by 46 publications

References 24 publications

Metabolic characterization of a strain (BM90) of Delftia tsuruhatensis showing highly diversified capacity to degrade low molecular weight phenols

Metabolic characterization of a strain (BM90) of Delftia tsuruhatensis showing highly diversified capacity to degrade low molecular weight phenols

Biosynthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer from wild-type Comamonas sp. EB172

Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by Burkholderia sacchari using wheat straw hydrolysates and gamma-butyrolactone

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