Biosynthesis and Structural Characteristics of Selenium Nanoparticles using Lactobacillus Acidophilus Bacteria by Wet Sterilization Process

Visha, P.; Nanjappan, K.; Selvaraj, Periasamy; Jayachandran, Seema; Elango, A.; Kumaresan, G

doi:10.23953/cloud.ijavst.183

Cited by 34 publications

(6 citation statements)

References 16 publications

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“…brevis CRL 2051; 10, Lb. plantarum CRL 2030; while lanes 11 and 17 correspond to F. tropaeoli CRL 2034. has been studied elsewhere (Eszenyi et al, 2011;Sasidharan and Balakrishnaraja, 2014;Visha et al, 2015;Moreno-Martin et al, 2017;Pescuma et al, 2017) showing also NP sizes between 40 and 500 nm depending on the strain used. Contrary to the majority of biosynthetic SeNPs like those reported by Moreno-Martin et al (2017), the nanoparticles produced by our strains were microcrystalline.…”

Section: Discussionmentioning

confidence: 96%

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Biotransformation of Selenium by Lactic Acid Bacteria: Formation of Seleno-Nanoparticles and Seleno-Amino Acids

Martínez

Moreno-Martín

Pescuma

et al. 2020

Front. Bioeng. Biotechnol.

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Selenium (Se) is an essential micronutrient for the majority of living organisms, and it has been identified as selenocysteine in the active site of several selenoproteins such as glutathione peroxidase, thioredoxin reductase, and deiodinases. Se deficiency in humans is associated with viral infections, thyroid dysfunction, different types of cancer, and aging. In several European countries as well as in Argentina, Se intake is below the recommended dietary Intake (RDI). Some lactic acid bacteria (LAB) can accumulate and bio-transform selenite (toxic) into Se-nanoparticles (SeNPs) and Se-amino acids (non-toxic). The microbial growth, Se metabolite distribution, and the glutathione reductase (involved in selenite reduction) activity of Se-enriched LAB were studied in this work. The ninety-six assayed strains, belonging to the genera Lactococcus, Weissella, Leuconostoc, Lactobacillus, Enterococcus, and Fructobacillus could grow in the presence of 5 ppm sodium selenite. From the total, eight strains could remove more than 80% of the added Se from the culture medium. These bacteria accumulated intracellularly between 1.2 and 2.5 ppm of the added Se, from which F. tropaeoli CRL 2034 contained the highest intracellular amount. These strains produced only the selenoamino acid SeCys as observed by LC-ICP-MS and confirmed by LC-ESI-MS/MS. The intracellular SeCys concentrations were between 0.015 and 0.880 ppm; Lb. brevis CRL 2051 (0.873 ppm), Lb. plantarum CRL 2030 (0.867 ppm), and F. tropaeoli CRL 2034 (0.625 ppm) were the strains that showed the highest concentrations. Glutathione reductase activity values were higher when the strains were grown in the presence of Se except for the F. tropaeoli CRL 2034 strain, which showed an opposite behavior. The cellular morphology of the strains was not affected by the presence of Se in the culture medium; interestingly, all the strains were able to form spherical SeNPs as determined by transmission electron microscopy (TEM). Only two Enterococcus strains produced the volatile Se compounds dimethyl-diselenide identified by GC-MS. Our results show that Lb. brevis CRL 2051, Lb. plantarum CRL 2030, and F. tropaeoli CRL 2034 could be used for the development of nutraceuticals or as starter cultures for the bio-enrichment of fermented fruit beverages with SeCys and SeNPs.

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Section: Discussionmentioning

confidence: 96%

“…Contrary to the majority of biosynthetic SeNPs like those reported by Moreno-Martin et al (2017), the nanoparticles produced by our strains were microcrystalline. Also, Visha et al (2015) reported a crystalline structure for the SeNPs produced by a Lb. acidophilus strain; these differences demonstrate the particle diversity produced by LAB.…”

Section: Discussionmentioning

confidence: 99%

Biotransformation of Selenium by Lactic Acid Bacteria: Formation of Seleno-Nanoparticles and Seleno-Amino Acids

Martínez

Moreno-Martín

Pescuma

et al. 2020

Front. Bioeng. Biotechnol.

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“…Increasing the concentration of NaHSeO 3 in the medium affected the growth of lactobacilli strains, according to González-Olivares et al (2016) who found that L. rhamnosus GG was more resistant to Na 2 SeO 3 at a concentration of 198 mg/L media; however, L. bulgaricus NCFB2772 showed the lowest assimilation of inorganic selenium (9.14%). Visha et al (2015) also indicated that L.…”

Section: Characterization Of Eco-friendly Senps In Powder Formmentioning

confidence: 97%

“…Several studies have prepared SeNPs using microorganisms (Eszenyi et al, 2011;Kheradmand et al, 2014;Porto et al, 2015Porto et al, , 2019Pusztahelyi et al, 2015;Visha et al, 2015), and the products were evaluated in terms of efficacy. Prokisch and Zommara (2011) produced high-quality SeNPs; Zommara et al (2018) produced biological SeNPs using yoghurt culture, which can be used safely in poultry nutrition and as a biogenic selenium source for humans.…”

Section: Introductionmentioning

confidence: 99%

Production and use of eco‐friendly selenium nanoparticles in the fortification of yoghurt

Salama

El‐Sayed

Abd-Rabou

et al. 2021

J. Food Process. Preserv.

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Eco‐friendly selenium nanoparticles (SeNPs) were prepared by biological‐methods using three individual or mixed strains of lactobacilli, which were evaluated for yield, selenium content, and particle size. The prepared SeNPs were used to fortify yoghurt, and the product was evaluated for its chemical composition, rheological properties, and microbiological quality. The highest SeNPs yield was obtained using Lactobacillus casei (26.87 g/L), whereas L. acidophilus produced nanoparticles with a significantly high selenium content. The lowest particle size (131.0 nm) was found in eco‐friendly SeNPs produced using mixed strains followed by those produced using L. plantarum (146.2 nm), L. acidophilus (202.8 nm), and L. casei (271.3 nm). Adding eco‐friendly SeNPs had no notable effect on the chemical composition and texture of functional yoghurt. Eco‐friendly SeNPs powder prepared with L. plantarum had the highest viable count, whereas the lowest was obtained using the mixed culture. No cytotoxicity was observed in a tested colon cancer cell line for all prepared eco‐friendly SeNPs. Practical applications The methods used in the preparation of selenium nanoparticles using lactic acid bacteria are safe, cheap, and easy to apply. It can be applied on an industrial scale as one of the ingredients that can be used to safely support different foods. Selenium nanoparticle prepared in this way can be added in the fortification of milk, children's food, and also bakery products to meet their daily needs of the important selenium element. They can also be produced in tablets containing the daily dose of selenium and also supported by probiotic bacteria. Therefore, the method used to prepare selenium nanoparticle in this study is one of the most promising ways to be applied in different food products.

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“…Nanostructures are more active than inorganic forms of selenium, they accumulate better in tissues, easily penetrate the cellular membrane and accumulate in the intracellular space [8,9]. Even at the very low selenium concentrations (0,005-0,1 %), its particles can adsorb antigens and antibodies on the surface.…”

Section: Food Science and Technologymentioning

confidence: 99%

Development of Biotechnology of Getting Selenium Nanostructures With Lactobacillus Acidophilus Culture

Kaprellyanz

Zykovа

Petrosyants

et al. 2018

EUREKA: Life Sciences

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Selenium nanostructures are characterized with high activity and antibacterial properties. Selenium nanoparticles, received by the biotechnological method using microorganisms are not toxic and free from admixtures of other toxic elements. For getting selenium nanostructures, there was set a problem of development of the biotransfrormation technology of inorganic selenium by the culture Lactobacillus acdophilus, strain 412/307 from the museum of the Department of Biochemistry, Microbiology and Physiology of Nutrition Odessa National Academy of Food Technologies (Ukraine). Selenium nanostructures are the side product in the process of selenocysteine formation. There was realized the study of the influence of the sodium selenite concentration on the growth speed and generation duration of lactobacteria on MRS medium for determining the main technological parameters. There was determined the quantitative content of accumulated selenium at different initial concentrations of sodium selenite in the medium. The important part of the elaborated technology is destruction of cellular walls of microorganisms for removal selenium nanoparticles. There was studied the influence of different physical-chemical factors of cells’ destruction. There was offered to use the combined influence of saline acid, freezing and microwave field for destructing cellular walls of lactobacteria biomasses. There is presented the scheme of the technology of getting “Nanoselenium” and given physical-chemical parameters of the received product.

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Biosynthesis and Structural Characteristics of Selenium Nanoparticles using Lactobacillus Acidophilus Bacteria by Wet Sterilization Process

Cited by 34 publications

References 16 publications

Biotransformation of Selenium by Lactic Acid Bacteria: Formation of Seleno-Nanoparticles and Seleno-Amino Acids

Biotransformation of Selenium by Lactic Acid Bacteria: Formation of Seleno-Nanoparticles and Seleno-Amino Acids

Production and use of eco‐friendly selenium nanoparticles in the fortification of yoghurt

Development of Biotechnology of Getting Selenium Nanostructures With Lactobacillus Acidophilus Culture

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