Biosynthesis, Characterization, and Evaluation of the Cytotoxic Effects of Biologically Synthesized Silver Nanoparticles from Cyperus conglomeratus Root Extracts on Breast Cancer Cell Line MCF-7

Al-Nuairi, Amani Ghassan; Mosa, Kareem A.; Mohammad, Mohammad G.; El‐Keblawy, Ali; Soliman, Sameh S. M.; Alawadhi, Hussain

doi:10.1007/s12011-019-01791-7

Cited by 46 publications

(15 citation statements)

References 48 publications

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“…This selectivity has been observed also with nanoparticles of other metals 29–31. It has been recently shown that silver nanoparticles green-synthetized using the desert plant Cyperus conglomeratus 31 or walnut green husk30 also present selectivity against cancer cells, without affecting non-cancerous cells.…”

Section: Discussionmentioning

confidence: 66%

“…Cell death analysis showed that CH-AuNPs have a selective cytotoxic effect in leukemic cells but not in PBMC nor in murine BM cells, at the same concentration and times, indicating that CH-AuNPs’ cytotoxicity is due to the characteristics of cancer cells, and not the cell type 10. This selectivity has been observed also with nanoparticles of other metals 29–31. It has been recently shown that silver nanoparticles green-synthetized using the desert plant Cyperus conglomeratus 31 or walnut green husk30 also present selectivity against cancer cells, without affecting non-cancerous cells.…”

Section: Discussionmentioning

confidence: 84%

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<p>Chitosan gold nanoparticles induce different ROS-dependent cell death modalities in leukemic cells</p>

Martínez‐Torres¹,

Lorenzo-Anota²,

García-Juárez³

et al. 2019

IJN

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BackgroundNanotechnology proposes the use of gold nanoparticles (AuNPs) for drug delivery, diagnosis, and treatment of cancer. Leukemia is a type of hematopoietic cancer that results from the malignant transformation of white blood cells. Chitosan-coated AuNPs (CH-AuNPs) are cell death inductors in HeLa and MCF-7 cancer cells without affecting peripheral blood mononuclear cells (PBMC). Considering the selectivity and versatile cytotoxicity of CH-AuNPs, we evaluated whether their selectivity is due to the cell lineage or the characteristics of the cancer cells, by assessing its cytotoxicity in leukemic cells. Moreover, we further examined the cell death mechanism and assessed the implication of nuclear damage, autophagosome formation, and the cell death mechanism induced in leukemic cells.Materials and methodsWe synthesized CH-AuNPs by chemical methods and analyzed their cell death capacity in a T-acute lymphocytic leukemia cell line (CEM), in a chronic myeloid leukemia cell line (K562), and in healthy cells from the same lineage (PBMC and bone marrow, BM, cells). Then, we assessed ROS generation and mitochondrial and nuclear damage. Finally, we evaluated whether cell death occurred by autophagy, apoptosis, or necroptosis, and the role of ROS in this mechanism.ResultsWe found that CH-AuNPs did not affect PBMC and BM cells, whereas they are cytotoxic in a dose-dependent manner in leukemic cells. ROS production leads to mitochondrial and nuclear damage, and cell death. We found that CH-AuNPs induce apoptosis in CEM and necroptosis in K562, both undergoing autophagy as a pro-survival mechanism.ConclusionCH-AuNPs are selective cell death inductors in hematologic cancer cells, without affecting their healthy counterparts. Cell death induced by CH-AuNPs is independent of the cancer cell type; however, its mechanism is different depending on the type of leukemic cells.

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Section: Discussionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 84%

<p>Chitosan gold nanoparticles induce different ROS-dependent cell death modalities in leukemic cells</p>

Martínez‐Torres¹,

Lorenzo-Anota²,

García-Juárez³

et al. 2019

IJN

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“…Various in vitro reports indicates that biogenic AgNPs decreased the mRNA and protein expression of MMP-2 and MMP-9 in wounded granulation tissues [27]. A huge amount of studies show treating MCF-7 cancer cells with AgNPs induces apoptosis by inducing the release of cytochrome c, production of reactive oxygen species [1], and activation of the caspase-3 pathway [28].…”

Section: Discussionmentioning

confidence: 99%

Anti-cancer, Anti-invasiveness & Anti-metastasis of Green Engineered Bioorganic-Capped Silver Nanoparticles Fabricated from Juniperus chinensis Extract and Comparison to Cisplatin in Lung Cancer Cells (A549): In Vitro Assessment of Cellular and Molecular Pathways

Noorbazargan

Mashayekhi

Khayam

et al. 2020

Preprint

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The current study reveals anti-cancer properties of bio-functionalized silver nanoparticle (AgNPs) fabricated by Juniperus chinensis leaf extracts due to it’s easy, low cost, biological activity, eco-friendly and lower side effects. The characteristics of AgNPs were determined by scanning electron microscopy [1], transmission electron microscopy (TEM), UV-visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction, Dynamic light scattering (DLS), Zeta potential and X-ray spectroscopy (EDX). Further, this study highlights the cellular and molecular mechanisms of AgNPs involves in anti-proliferative and apoptotic effect on human lung cancer cells (A549) and compared to commercial drug cisplatin by various biological methods such as MTT, flow cytometery analysis, gene expression patterns, migration and invasion inhibition, ROS and caspase production and cell staining. The size of AgNPs fabricated in this study was 12.96 with spherical shape and negative zeta potential. Up-regulation of capase 3,9 and p53, Annexin V-FITC/PI, DAPI staining, ROS production indicated remarkable apoptotic effect of AgNPs than cisplatin. Also down-regulation of MMP2/MMP9 scratch and matrigel assays revealed anti-metastatic properties of AgNPs.cell cycle analysis and down regulation of cyclin D1 showed cancer cell cessation in the G0/G1 phase. Overall results in current experiment revealed AgNPs synthetized by biogreen method anti-metastasis and anti-proliferation effect on lung cancer cells comparison to cicplatin drug and also had lower side effect on normal cell line.

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“…The samples were placed on standard carbon-coated copper grids (200-mesh) and air-dried for about 2h prior to TEM measurement. The energy-dispersive X-ray spectroscopy (Oxford Instruments X-Max 50 EDS detector) was carried out according to Al-Nuairi et al [ 13 ]. X-ray diffraction (XRD) measurements were carried out using Cu radiation on a Bruker D8 Advance Powder Diffractometer.…”

Section: Methodsmentioning

confidence: 99%

Effective targeting of breast cancer cells (MCF7) via novel biogenic synthesis of gold nanoparticles using cancer-derived metabolites

et al. 2020

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Biogenic synthesis of nanoparticles provides many advantages over synthetic nanoparticles including clean and non-toxic approaches. Nanoparticle-based application for the development of diagnostics and therapeutics is a promising field that requires further enrichment and investigation. The use of biological systems for the generation of gold nanoparticles (AuNPs) has been extensively studied. The search for a biocompatibility approach for the development of nanoparticles is of great interest since it can provide more targeting and less toxicity. Here, we reported a bio-reductive approach of gold to AuNPs using metabolites extracted from mammalian cells, which provided a simple and efficient way for the synthesis of nanomaterials. AuNPs were more efficiently synthesized by the metabolites extracted from breast cancer (MCF7) and normal fibroblasts (F180) cells when compared to metabolites extracted from cell-free supernatants. The metabolites involved in biogenic synthesis are mainly alcohols and acids. Spectroscopic characterization using UV-visible spectra, morphological characterization using electron microscopy and structural characterization using X-ray diffraction (XRD) confirmed the AuNPs synthesis from mammalian cells metabolites. AuNPs generated from MCF7 cells metabolites showed significant anticancer activities against MCF7 and low toxicity when compared to those generated from F180 cells metabolites. The results reflected the cytotoxic activities of the parent metabolites extracted from MCF7 versus those extracted from F180. Comparative metabolomics analysis indicated that MCF7-generated AuNPs harbored tetratetracontane, octacosane, and cyclotetradecane while those generated from F180 harbored a high percentage of stearic, palmitic, heptadecanoic acid. We related the variation in cytotoxic activities between cell types to the

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Biosynthesis, Characterization, and Evaluation of the Cytotoxic Effects of Biologically Synthesized Silver Nanoparticles from Cyperus conglomeratus Root Extracts on Breast Cancer Cell Line MCF-7

Cited by 46 publications

References 48 publications

<p>Chitosan gold nanoparticles induce different ROS-dependent cell death modalities in leukemic cells</p>

<p>Chitosan gold nanoparticles induce different ROS-dependent cell death modalities in leukemic cells</p>

Anti-cancer, Anti-invasiveness & Anti-metastasis of Green Engineered Bioorganic-Capped Silver Nanoparticles Fabricated from Juniperus chinensis Extract and Comparison to Cisplatin in Lung Cancer Cells (A549): In Vitro Assessment of Cellular and Molecular Pathways

Effective targeting of breast cancer cells (MCF7) via novel biogenic synthesis of gold nanoparticles using cancer-derived metabolites

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Biosynthesis, Characterization, and Evaluation of the Cytotoxic Effects of Biologically Synthesized Silver Nanoparticles from Cyperus conglomeratus Root Extracts on Breast Cancer Cell Line MCF-7

Cited by 46 publications

References 48 publications

<p>Chitosan gold nanoparticles induce different ROS-dependent cell death modalities in leukemic cells</p>

<p>Chitosan gold nanoparticles induce different ROS-dependent cell death modalities in leukemic cells</p>

Anti-cancer, Anti-invasiveness &amp; Anti-metastasis of Green Engineered Bioorganic-Capped Silver Nanoparticles Fabricated from Juniperus chinensis Extract and Comparison to Cisplatin in Lung Cancer Cells (A549): In Vitro Assessment of Cellular and Molecular Pathways

Effective targeting of breast cancer cells (MCF7) via novel biogenic synthesis of gold nanoparticles using cancer-derived metabolites

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Product

Resources

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Anti-cancer, Anti-invasiveness & Anti-metastasis of Green Engineered Bioorganic-Capped Silver Nanoparticles Fabricated from Juniperus chinensis Extract and Comparison to Cisplatin in Lung Cancer Cells (A549): In Vitro Assessment of Cellular and Molecular Pathways