Biosynthesis, characterization, magnetic hyperthermia, and in vitro toxicity evaluation of quercetin-loaded magnetoliposome lipid bilayer hybrid system on MCF-7 breast cancer

Elbeltagi, Shehab; Saeedi, Ahmad M.; Eldin, Zienab E.; Alfassam, Haifa E.; Alharbi, Hanan M.; Madkhali, Nawal; Shakor, Abo Bakr Abdel; El-Aal, Mohamed Abd

doi:10.1016/j.bbagen.2023.130543

Cited by 11 publications

(2 citation statements)

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“…In TEM, NG-loaded MOF-5@LNs surface morphology is bene cial for drug release, which is especially important for biological applications [64][65][66][67][68][69][70][71]. Author et al [42], the spherical shape of drug-loaded NPs@liposomes could be attributed to which form of closed bilayer vesicles in water reduced their surface-free energy. The combination of NG-MOF-5@LNs may result in intricate interactions, which may be the cause of the lyophilized dry microparticles minor deformation [51,72,73].…”

Section: Discussionmentioning

confidence: 99%

“…After that, 96-well plates were lled with the three copies of the substances under investigation (NG, MOF-5, and NG-MOF-5@LNs) to create 12 concentrations of each NG. Six vehicle controls comprising medium or 0.5% DMSO were utilized as a control for every 96-well plate [42,43]. After incubating for 24h, the number of viable cells was determined by the MTT method.…”

Section: Mtt Assaymentioning

confidence: 99%

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Investigation and optimization of naringin-loaded in MOF-5 encapsulated by liponiosomes as smart drug delivery, cytotoxicity, and apoptotic on breast cancer cells

Elbeltagi,

Alharbi,

Aodah

et al. 2024

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Tumors are considered dangerous due to their significant global mortality rate, with breast cancer (BC) being a major contributor to this statistic, impacting almost all tumor patients worldwide. Conventional methods for BC treatment, such as special naringin (NG) chemotherapy based on nanotechnology components, have been insufficient, and a new approach has been used to improve drug delivery (DD) to cancer. Herein, developed biocompatible nanoparticles (NPs) comprising NG-loaded zinc metal-organic framework-5 (NG-MOF-5) encapsulated with liponiosomes (LNs) to create NG-MOF-5@LNs. Various biophysical characteristics of free NG, MOF-5, and NG-MOF-5@LNs NPs were studied, including XRD, FTIR, TEM, SEM, zeta potential (ZP), hydrodynamic size, PDI, loading efficiency (LE), encapsulation efficiency (EE), drug release (DR), and drug-release kinetics. TEM images showed that NG-MOF-5@LNs NPs consisted of monodispersed spherical-like particles with average diameters of 122.5 nm (*P < 0.05). The optimized NG-MOF-5@LNs exhibited a particle size (PDI: 139.8 nm) and a ZP (+ 21 mV). The EE and LE for NG-MOF-5@LNs were 89.15% and 14.05%, respectively. Four kinetic models were examined to understand the NG release mechanism at pH 6.9, with the Higuchi model indicating excellent fit (R2 = 0.9988). The MTT cell viability assay demonstrated anti-cancer activity against MCF-7 BC cells, with inhibitory concentrations (IC50) of 231.9, 188.2, and 21.6 µg/mL (*P < 0.05) for MOF-5, NG, and NG-MOF-5@LNs, respectively, indicating an apoptosis effect on MCF-7 cells. NG-MOF-5@LNs is expected to emerge as a novel long-term-release anticancer agent. NG-MOF-5@LNs may represent a superior option for BC therapy compared to conventional NG formulations.

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