Biosynthesis of Copper Nanoparticles Using Parthenium Hysterophorus Leaf Extract and Screening Its Antimicrobial Activity

Abstract

Objective: The aim of this study is to synthesis the copper nanoparticles (CuNPs) using the leaf extract of Parthenium hysterophorus.
 Methods: Dry and fresh leaf extract was prepared and CuSo4 was added. The color change was noted and recorded by ultraviolet–visible spectrophotometer. The morphological characteristics were analyzed by scanning electron microscopy (SEM). Antimicrobial activities were performed by the disc diffusion method.
 Results: The color change indicates the production of CuNPs. Surface plasmon resonance band was observed around 599 nm and 572 nm for fresh and dry samples of P. hysterophorus leaf extract. SEM confirms the formation and the crystalline nature of CuNPs and X-ray diffraction studies show the particle size. The antibacterial potentials of the CuNPs were studied and have shown good high inhibition activity against Staphylococcus aureus, Bacillus subtilis, Proteus vulgaris, and Pseudomonas aeruginosa at different concentrations in compare to fungi species.
 Conclusion: This method is effective and environmental friendly for the synthesis of CuNPs using leaf extract of Parthenium hysterophorus.