Biosynthesis of polyhydroxyalkanoates from styrene by Enterobacter spp. isolated from polluted environment

Arshad, Arooj; Ashraf, Bisma; Ali, Iftikhar; Jamil, Nazia

doi:10.1007/s11515-017-1446-2

Cited by 17 publications

(16 citation statements)

References 28 publications

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“…This successful result may indicate that initial cell growth should rst be achieved before PHA production, preferably reducing the cytotoxicity of biphenyl. Regarding the molecular weight of the polymer product, much higher values were obtained than those obtained in the other cases such as the conversion of aromatic hydrocarbon styrene to PHA (Ward et al 2005;Tan et al 2015;Arshad et al 2017). It is of interest to address the reason behind this in our microbial conversion system.…”

Section: Discussionmentioning

confidence: 91%

Bioconversion of biphenyl to a polyhydroxyalkanoate copolymer by Alcaligenes denitrificans A41

Yajima

Nagatomo

Wakabayashi

et al. 2020

Preprint

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A polyhydroxyalkanoate (PHA) copolymer, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)], was biosynthesized from biphenyl as the sole carbon source using Alcaligenes (currently Achromobacter) denitrificans A41. This strain is capable of degrading polychlorinated biphenyls (PCBs) and biphenyl. This proof-of-concept of the conversion of aromatic chemicals such as the environmental pollutant PCBs/biphenyl to eco-friendly products such as biodegradable polyester PHA was inspired by the uncovering of two genes encoding PHA synthases in the A. denitrificans A41 genome. When the carbon/nitrogen (C/N) ratio was set at 21, the cellular P(3HB-co-3HV) content in strain A41 reached its highest value of 10.1% of the cell dry weight (CDW). A two-step cultivation protocol improved the accumulation of P(3HB-co-3HV) by up to 26.2% of the CDW, consisting of 13.0 mol% 3HV when grown on minimum salt medium without nitrogen sources. The highest cellular content of P(3HB-co-3HV) (47.6% of the CDW) was obtained through the two-step cultivation of strain A41 on biphenyl as the sole carbon source. The purified copolymer had ultra-high molecular weight (weight-average molecular weight of 3.5 × 106), as revealed through gel-permeation chromatography. Based on the genomic information related to both polymer synthesis and biphenyl degradation, we finally proposed a metabolic pathway for the production of P(3HB-co-3HV) associated with the degradation of biphenyl by strain A41.

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Section: Discussionmentioning

confidence: 91%

Bioconversion of biphenyl to a polyhydroxyalkanoate copolymer by Alcaligenes denitrificans A41

Yajima

Nagatomo

Wakabayashi

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…The cellular content and molar fraction of the P(3HB-co-3HV) copolymer were measured using GC obtained in the other cases such as the conversion of aromatic hydrocarbon styrene to PHA (Ward et al 2005;Tan et al 2015;Arshad et al 2017). It is of interest to address the reason behind this in our microbial conversion system.…”

Section: Discussionmentioning

confidence: 99%

Bioconversion of biphenyl to a polyhydroxyalkanoate copolymer by Alcaligenes denitrificans A41

et al. 2020

View full text Add to dashboard Cite

A polyhydroxyalkanoate (PHA) copolymer, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)], was biosynthesized from biphenyl as the sole carbon source using Alcaligenes (currently Achromobacter) denitrificans A41. This strain is capable of degrading polychlorinated biphenyls (PCBs) and biphenyl. This proof-of-concept of the conversion of aromatic chemicals such as the environmental pollutant PCBs/biphenyl to eco-friendly products such as biodegradable polyester PHA was inspired by the uncovering of two genes encoding PHA synthases in the A. denitrificans A41 genome. When the carbon/nitrogen (C/N) ratio was set at 21, the cellular P(3HB-co-3HV) content in strain A41 reached its highest value of 10.1% of the cell dry weight (CDW). A two-step cultivation protocol improved the accumulation of P(3HB-co-3HV) by up to 26.2% of the CDW, consisting of 13.0 mol % 3HV when grown on minimum salt medium without nitrogen sources. The highest cellular content of P(3HB-co-3HV) (47.6% of the CDW) was obtained through the two-step cultivation of strain A41 on biphenyl as the sole carbon source. The purified copolymer had ultra-high molecular weight (weight-average molecular weight of 3.5 × 10 6), as revealed through gel-permeation chromatography. Based on the genomic information related to both polymer synthesis and biphenyl degradation, we finally proposed a metabolic pathway for the production of P(3HB-co-3HV) associated with the degradation of biphenyl by strain A41.

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“…The mixture was left for cooling and then centrifuged at 10 3 rpm for 20 minutes. The extract was condensed using a rotary evaporator at 50°C and then added dropwise into 10 volumes of cold methanol with continuous stirring . Thereafter, the extracted PHAs were dried in an oven and calculated as a percentage of the dried cell biomass.…”

Section: Methodsmentioning

confidence: 99%

Production of butanol and polyhydroxyalkanoate from industrial waste byClostridium beijerinckiiASU10

et al. 2019

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Summary This study demonstrated a biotechnological approach for simultaneous production of low‐cost H2, liquid biofuels, and polyhydroxyalkanoates (PHAs) by solventogenic bacterium (Clostridium beijerinckii) from renewable industrial wastes such as molasses and crude glycerol. C beijerinckii ASU10 (KF372577) exhibited considerable performance for hydrogen production of 5.1 ± 0.84 and 11 ± 0.44 mL H2 h−1 on glycerol and sugarcane molasses, respectively. The total acetone‐butanol‐ethanol (ABE) generation from glycerol and molasses was 9.334 ± 2.98 and 10.831 ± 4.1 g L−1, respectively. ABE productivity (g L−1 h−1) was 0.0486 and 0.0564 with a yield rate (g g−1) up to 0.508 and 0.493 from glycerol and molasses fermentation, respectively. The PHA yields from glycerol and sugarcane molasses were 84.37% and 37.97% of the dried bacterial biomass, respectively. Additionally, the ultrathin section of C beijerinckii ASU10 showed that PHA granules were accumulated more densely on glycerol than molasses. Gas chromatography–mass spectrometry (GC‐MS) analysis confirmed that the PHAs obtained from molasses fermentation included 3‐hydroxybutyrate (47.3%) and 3‐hydroxyoctanoate (52.7%) as the main constituents. Meanwhile, 3‐hydroxybutyrate represented the sole monomer of PHA produced from glycerol fermentation. This study demonstrated that C beijerinckii ASU10 (KF372577) is a potent strain for low‐cost PHA production depending on its high potential to produce high‐energy biofuel and other valuable compounds from utilization of organic waste materials.

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Biosynthesis of polyhydroxyalkanoates from styrene by Enterobacter spp. isolated from polluted environment

Cited by 17 publications

References 28 publications

Bioconversion of biphenyl to a polyhydroxyalkanoate copolymer by Alcaligenes denitrificans A41

Bioconversion of biphenyl to a polyhydroxyalkanoate copolymer by Alcaligenes denitrificans A41

Bioconversion of biphenyl to a polyhydroxyalkanoate copolymer by Alcaligenes denitrificans A41

Production of butanol and polyhydroxyalkanoate from industrial waste byClostridium beijerinckiiASU10

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