Biosynthesis of silver nanoparticles using stem bark extracts of Diospyros montana and their antioxidant and antibacterial activities

Abstract

The present study reports an eco-friendly, biosynthesis of silver nanoparticles (AgNPs) using stem bark extract of Diospyros montana. Initially, the synthesis of AgNPs was confirmed by visual observation as color change. Further, the morphology of the biosynthesized nanoparticles, average size and presence of elemental silver were characterized by UV-Visible spectroscopy, scanning electron microscopy, transmission electron microscopy, energy dispersive X-ray and dynamic light scattering spectrometer. Qualitative phytochemical screening and FTIR spectral peaks supported the role of phytochemicals in bark extract for the metal reduction, stabilization and capping of silver nanoparticles. XRD studies demonstrated that crystalline nature and their average size of nanoparticles was 28 nm as determined by Scherrer's formula. The antioxidant ability of AgNPs and plant extract was analyzed using DPPH and Hydrogen peroxide assay. The percentage of DPPH and H 2 O 2 activity was increased with increasing concentration of AgNPs. In vitro antibacterial effect of various concentration of AgNPs was investigated against both Gram positive (B.subtilis and S.aureus) and Gram negative (E.coli and K.aerogenes) bacterial strains. The result shows that biosynthesized AgNPs have significant antibacterial activity.

Preparation and characterization of ascorbic acid-mediated chitosan–copper oxide nanocomposite for anti-microbial, sporicidal and biofilm-inhibitory activity

“…Thus, the formation of CuO nanoparticles is confirmed, and Cu is the main element in synthesized nanoparticles. Other absorbance peaks of O and C in the spectrum obtained were mostly due to the emission of X-ray from existing O-and C-related compounds of ascorbic acid and chitosan [5]. Figure 4 shows the XRD diffraction pattern of the prepared Chi-CuO nanocomposites.…”

“…6 and 7 clearly shows that the major spectral peaks of the prepared Chi-CuO composites are derived from ascorbic acid and chitosan. The presence of these bio and polymer compounds may be responsible for the reduction and conversion of synthesized metal nanocomposites (Chi-CuO) [5]. …”

“…Green synthesis of metal and metal oxide nanoparticles using plants, microorganisms and bio-compounds is environmental friendly without using toxic compounds and involves simple preparation methods [5].…”

Characterization and anti‐acute T cell leukemia properties of silver nanoparticles synthesized by a green approach for bioremediation applications: Introducing a new chemotherapeutic drug for clinical trial studies

“…As to the methods of obtaining silver nanoparticles, different strategies were successfully used, thanks to the intrinsic versatility of silver metal and silver-based compounds, including physical, chemical, physicochemical, and biological synthesis approaches. [8][9][10] However, given the facile and safe process, reduced economic implications, and repeatability and reproducibility of experimental results, the method most used in the preparation of AgNPs is represented by the chemical reduction of silver salts by sodium citrate or sodium borohydrate. [11,12] In addition to their intrinsic antimicrobial-related applications, AgNPs were thoroughly explored thanks to their beneficial size-related physicochemical effects exhibited in novel electronic, magnetic, catalytic, and optical materials.…”

“…[11,12] In addition to their intrinsic antimicrobial-related applications, AgNPs were thoroughly explored thanks to their beneficial size-related physicochemical effects exhibited in novel electronic, magnetic, catalytic, and optical materials. [9][10][11] Special interest is oriented toward improving the stability of AgNPs, since a particular limitation of their antimicrobial-related use arises from their instability in bacteria-rich environments, and consequently, diminution or deprivation of their anti-pathogenic activity. [11][12][13] In order to improve the stability of AgNPs in solution, many inorganic and organic, synthetic and natural, and biotic and abiotic materials were used as capping agents.…”