Biosynthesized silver nanoparticles using Caulerpa taxifolia against A549 lung cancer cell line through cytotoxicity effect/morphological damage

Abstract

The Caulerpa taxifolia is excellent marine green algae, which produced enormous bioactive compounds with more biological activities. Also, it is an excellent source for synthesis of Ag NPs with increased bioactivity against various infections. In our study, the marine algae marine algae Caulerpa taxifolia mediated Ag NPs was synthesized effectively. The synthesized Ag NPs was characterized well using UV-spectrometer and X-ray powder diffraction (XRD) and confirmed as synthesized particle was Ag NPs. The available structure of the Ag NPs was morphologically identified by scanning electron microscope (SEM), and exact minimum size, polydispersive spherical shape of the entire Ag NPs structure was confirmed by Transmission electron microscope (TEM). Further, the anti-cancer efficiency of biosynthesized Ag NPs against A549 lung cancer cells was found at 40 µg/mL concentration by cytotoxicity experiment. In addition, the phase contrast images of the result were supported the Ag NPs, which damaged the A549 morphologically clearly. Finally, florescence microscopic images were effectively proved the anti-cancerous effect against A549 lung cancer cells due to the condensed morphology of increased death cells. All the confirmed in-vitro results were clearly stated that the Caulerpa taxifolia mediated Ag NPs has superior anti-cancer agent against A549 lung cancer cells.

Biogenic Preparation, Characterization, and Biomedical Applications of Chitosan Functionalized Iron Oxide Nanocomposite

“…Prepared CS/FeO NC induced 50% (IC 50 ) of growth suppression property at the concentration of 82 ± 0.50 µg/mL. Similar to our study, biosynthesized silver nanoparticles from Caulerpa taxifolia also showed the concentration-dependent anti-lung cancer activity [48]. In addition, the A549 cell proliferation was observed using fluorescence microscopy.…”

Lung Models to Evaluate Silver Nanoparticles’ Toxicity and Their Impact on Human Health

“…Interestingly, these works are all related to the cytotoxicity evaluation of the A549 cell line. Several studies reported lung cell cytotoxicity in a concentration-dependent manner for small-sized [45,47,49,52] and big-sized [48] AgNPs. Some other authors disclose the time and size-dependent cytotoxicity for this particular cell line for medium-sized AgNPs [50,51].…”

“…The differential gene expression observed among several lung cell types exposed to different AgNP formulations indicates that independently of the coating, size, or cell type, AgNPs impact transcriptional reprogramming of exposed cells. The specific gene expression changes of lung cells exposed to AgNPs can be used as inflammation or lung damage biological biomarkers [14,29,48,49,56,72,73,148,152]. Moreover, AgNPs exert cellular lung death by altering cell cycle, DNA content, and membrane tissue remodeling, further deriving cell death in a cell-type dependent manner.…”

Marine-Bioinspired Nanoparticles as Potential Drugs for Multiple Biological Roles

“…Marine-derived natural products, in particular, are potential molecules for the development of anticancer drugs because they may influence multiple pathways, such as immunity, cancer cell death, and tumor growth [ 118 ]. Silver nanoparticles derived from Caulerpa taxifolia showed antitumor efficacy against A549 lung cancer cells [ 119 ]. Necrosis and condensation of A549 cells were shown to be mediated by silver nanoparticles derived from marine algae, suggesting that nanomaterials are relevant for cancer cell research.…”