Biosynthetic hydrogel scaffolds made from fibrinogen and polyethylene glycol for 3D cell cultures

Almany, Liora; Seliktar, Dror

doi:10.1016/j.biomaterials.2004.06.047

Cited by 514 publications

(381 citation statements)

References 30 publications

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“…Prehydrogel Solution Preparation for 3D‐Bioprinting : Crosslinkable PEGDA of different molecular weights ( M w = 4K and 10K) were synthesized as previously described 9. The recipes for preparing prehydrogel solutions are listed in Table S1 in the Supporting Information.…”

Section: Methodsmentioning

confidence: 99%

3D‐Bioprinted Osteoblast‐Laden Nanocomposite Hydrogel Constructs with Induced Microenvironments Promote Cell Viability, Differentiation, and Osteogenesis both In Vitro and In Vivo

et al. 2017

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An osteoblast‐laden nanocomposite hydrogel construct, based on polyethylene glycol diacrylate (PEGDA)/laponite XLG nanoclay ([Mg5.34Li0.66Si8O20(OH)4]Na0.66, clay)/hyaluronic acid sodium salt (HA) bio‐inks, is developed by a two‐channel 3D bioprinting method. The novel biodegradable bio‐ink A, comprised of a poly(ethylene glycol) (PEG)–clay nanocomposite crosslinked hydrogel, is used to facilitate 3D‐bioprinting and enables the efficient delivery of oxygen and nutrients to growing cells. HA with encapsulated primary rat osteoblasts (ROBs) is applied as bio‐ink B with a view to improving cell viability, distribution uniformity, and deposition efficiency. The cell‐laden PEG–clay constructs not only encapsulated osteoblasts with more than 95% viability in the short term but also exhibited excellent osteogenic ability in the long term, due to the release of bioactive ions (magnesium ions, Mg2+ and silicon ions, Si4+), which induces the suitable microenvironment to promote the differentiation of the loaded exogenous ROBs, both in vitro and in vivo. This 3D‐bioprinting method holds much promise for bone tissue regeneration in terms of cell engraftment, survival, and ultimately long‐term function.

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Section: Methodsmentioning

confidence: 99%

3D‐Bioprinted Osteoblast‐Laden Nanocomposite Hydrogel Constructs with Induced Microenvironments Promote Cell Viability, Differentiation, and Osteogenesis both In Vitro and In Vivo

et al. 2017

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“…They are already recognized to be excellent for 3D micro and nanostructuring. ORMOCER (Ormocore b59, Microresist GmbH) and biodegradable PEG-DA (Poly-Ethylen Glycol DiAcrylate Mn 258, Sigma-Aldrich Co.) are known to be biocompatible materials and have been used as the reference photopolymers for determining the biocompatibility from the cell growth [24,25].…”

Section: Fabrication Of the Scaffoldsmentioning

confidence: 99%

3D artificial polymeric scaffolds for stem cell growth fabricated by femtosecond laser

Malinauskas

Danilevičius²,

Baltriukienė³

et al. 2010

Lithuanian J. Phys.

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Femtosecond Laser Induced Polymerization is an attractive direct writing technique for rapid three-dimensional (3D) micro and nanofabrication in diverse applications. Recently, it has been successfully applied for 3D scaffold fabrication required in biomedicine applications. However, there are still a lot of investigations to be done before it can be used for practical applications in tissue engineering or regenerative medicine. In this work, experimental results on production of artificial polymeric scaffolds for stem cell growth are presented. Parameters (average laser power, sample scanning speed, and developing conditions) for microfabrication in biocompatible photopolymers AKRE (AKRE37) and ORMOSIL (SZ2080) are experimentally determined. 3D custom form and size artificial scaffolds were successfully microfabricated. Adult stem cell growth on them was investigated in order to test their biocompatibility. The results of myogenic stem cell culture expansion were compared to the control growth of the same cells on the scaffolds manufactured out of commonly used biocompatible photopolymers ORMOCER (Ormocore b59) and Poly-Ethylen Glycol Di-Acrylate (PEG-DA-258). Preliminary results show FLIP technique to have potential in fabrication of artificial 3D polymeric scaffolds for cell proliferation experiments. These are the first steps in transferring FLIP fabrication method from laboratory tests to flexible manufacturing of individual scaffolds out of biocompatible and biodegradable polymers.

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“…The main advantage of PEGylated natural protein biomaterials [21][22][23][24][25][26][27][28] is their unique features. The synthetic PEG provides the biophysical and structural properties of the hydrogel.…”

Section: Introductionmentioning

confidence: 99%

“…The fibrinogen directs the biological response based on biological cell signaling domains on the protein that are tissue repair specific [29]. These signals encourage migration, attachment and differentiation of endothelial cells and smooth muscle cells [21,30]. Additionally, the fibrinogen affords the scaffold proteolytic sensitivity for in situ biodegradation by cell activated protease activity [31].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Biocompatibility of Pegylated Fibrinogen and Its Effect on Healing of Full-Thickness Skin Defects: A Preliminary Study in Rats

CM¹,

Jacot²

2016

J Biotechnol Biomater

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Citation: Venzin CM, Jacot V, Berdichevsky A, Karol AA, Seliktar D, et al. (2016) AbstractIntroduction: A synthetic polymer polyethylene glycol (PEG), was conjugated to fibrinogen as a threedimensional and biodegradable skin wound dressing matrix. This PEG-fibrinogen (PEG-fib) was tested in vivo in a skin wound time course study for its biocompatibility and biodegradation, after being delivered into the wound by injection and polymerized in situ by photo-activation.

show abstract

Biosynthetic hydrogel scaffolds made from fibrinogen and polyethylene glycol for 3D cell cultures

Cited by 514 publications

References 30 publications

3D‐Bioprinted Osteoblast‐Laden Nanocomposite Hydrogel Constructs with Induced Microenvironments Promote Cell Viability, Differentiation, and Osteogenesis both In Vitro and In Vivo

3D‐Bioprinted Osteoblast‐Laden Nanocomposite Hydrogel Constructs with Induced Microenvironments Promote Cell Viability, Differentiation, and Osteogenesis both In Vitro and In Vivo

3D artificial polymeric scaffolds for stem cell growth fabricated by femtosecond laser

Biocompatibility of Pegylated Fibrinogen and Its Effect on Healing of Full-Thickness Skin Defects: A Preliminary Study in Rats

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