Biosynthetic Studies on the Collagenous Components of Basement Membranes

Jg, Heathcote; Muhammed, Selahattin; Ef, Smith; Me, Grant

doi:10.1159/000172739

Cited by 4 publications

(2 citation statements)

References 9 publications

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“…It is thought to have a collagenous framework bound by hydroxylysine linkages to disaccharide units, forming a gel (Spiro 1967). A similar structure has been proposed for the basement membrane of the lens (Veis & Schwartz 1981), although glomerular basement membrane has a more complex polypeptide composition than has lenticular basement membrane (Heathcote et at. 1980).…”

Section: The Structure Of Basement Membrane In Relation To Its Permeabilitymentioning

confidence: 58%

The water permeability of basement membrane under increasing pressure: evidence for a new theory of permeability

1982

Proc. R. Soc. Lond. B.

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The water permeability and physical characteristics of the basement membrane (lens capsule) of the crystalline lens of the adult rat have been investigated. The hydraulic conductivity of the basement membrane at low pressure is 50 ± 9.5 x 10 -12 m s -1 Pa -1 and at high pressure 17 ± 7.5 x 10 -12 m s -1 Pa -1 . This decrease in permeability occurs despite a 75% increase in area of the membrane and a 65% reduction in its thickness. Conventional theories of a membrane possessing pores or of a fibre matrix of filaments of a constant diameter fail to explain the decreasing permeability of the membrane with increasing hydrostatic pressure. The present data suggest that the structure of the membrane is changed by pressure and the coiled filaments of which it is composed are extended as stress is applied to the membrane. If allowance is made both for thinning and for compaction of the membrane and the extension of its area the permeability of the membrane can be predicted satisfactorily at varying pressures. Thus the hydraulic conductivity of basement membrane at a given pressure can be adequately described by the product of a constant and a dimensionless ‘deformation coefficient’. This deformation coefficient is equal to the square of the product of the thickness ratio and elongation ratio of the membrane at the given filtration pressure.

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Section: The Structure Of Basement Membrane In Relation To Its Permeabilitymentioning

confidence: 58%

The water permeability of basement membrane under increasing pressure: evidence for a new theory of permeability

1982

Proc. R. Soc. Lond. B.

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“…The 180,000 and 160,000 molecular weight components may be the al(IV) and a2(lV) chains which have been described in GB M from other species [20]. If this is the case, the variation in band intensity with age would have to be explained by differential turnover or synthesis rather than posl-lranslational modification of the procollagen chains [27], Similar complexity in band pat tern in the pro-a-region has also been noted in human [21; Fish, personal commun.] and steer GBM [28].…”

Section: Discussionmentioning

confidence: 95%

Age-Related Changes in Rat Glomerular Basement Membrane Components Solubilised with Pepsin

Khalil-Manesh¹,

Price²

1985

Kidney Blood Press Res

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The proportion of glomerular basement membrane (GBM) solubilised following pepsin treatment increased with age when the digestion was carried out at 4°C, but remained constant at 50% of the membrane when the digestion was carried out at 10°C irrespective of rat age. Amino-acid analysis of the solubilised material indicated that it became more collagen-like with age, as judged by the increase in glycine, hydroxyproline and hydroxylysine content, if the digestion was carried out at 10 °C. Polyacrylamide gel electrophoresis of pepsin-solubilised material demonstrated that components with apparent molecular weights of 180,000, 160,000 and 130,000 increased in intensity with age. Components with apparent molecular weights < 85,000 were only present in pepsin-soluble material prepared from GBM isolated from young rats. The intensity of the bands in the pro-α-region varied with age and some procollagen-like material was not solubilised by pepsin treatment. These studies confirm that aging of rat GBM is characterised by an increase in the proportion of the collagenous components present in rat GBM.

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Cell associated glycoproteins synthesized by cultured renal tubular cells

1982

Histochemistry

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Thin cortical kidney explants from newborn New Zealand rabbits were cultured in Dulbecco's MEM containing 10% fetal bovine serum. Within 24 h the explants formed globular bodies which were completely covered by a monolayered epithelium. The cells show polar differentiation and resemble the renal collecting duct epithelium. By culturing the globular bodies in Dulbecco's MEM with D-valine instead of L-valine additionally a monolayer of renal collecting duct cells was obtained. For the study of glycoprotein synthesis the globular bodies and the collecting duct monolayers were incubated with various labelled carbohydrates, protein and collagen precursors and then fractionated into coarse membrane pellets. The synthesized glycoproteins were regained in 600 x g and 12,000 x g coarse membrane fractions and extracted with Triton X 100 buffer for column chromatography and SDS-polyacrylamide electrophoresis in 6 M urea. In addition to a 85,000 d glycoprotein, a carbohydrate rich collagen like protein (apparent molecular weight in column chromatography 200,000 d, in the SDS-polyacrylamide electrophoresis 150,000 d) was found. The 150,000 d glycoprotein incorporates favorably radioactive proline, sulfate, and smaller amounts of lysine, and leucine. Compared to the 85,000 d glycoprotein a double amount of glucosamine and galactose and four fold amount of fucose was detected. The 85,000 d protein has to be ascribed as a usual glycoprotein, in contrast the 150,000 d protein shows an unusual combination of characteristics and has to be considered as a new type of renal glycoprotein.

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Biosynthetic Studies on the Collagenous Components of Basement Membranes

Cited by 4 publications

References 9 publications

The water permeability of basement membrane under increasing pressure: evidence for a new theory of permeability

The water permeability of basement membrane under increasing pressure: evidence for a new theory of permeability

Age-Related Changes in Rat Glomerular Basement Membrane Components Solubilised with Pepsin

Cell associated glycoproteins synthesized by cultured renal tubular cells

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