Biosynthetic studies through feeding experiments in Eclipta prostrata (L.) L. hairy roots

Abstract

Eclipta prostrata (L.) L. is a medicinal plant of the Asteraceae family, and several extracts and isolated compounds of E. prostrata (L.) L. showed a wide range of biological activities such as antimicrobial, anticancer, hepatoprotective, neuroprotective, hair growth promoting activities, and more recently against covid-19. Eclipta prostrata (L.) L. hairy roots produce wedelolactone (WL), demethylwedelolactone (DWL) and 3,5-di- O -caffeoylquinic acid (3,5-diCQA), and there is no data in literature regarding biosynthetic pathways are involved. To verify the biosynthetic route, feeding experiments were carried out using sodium [2- 13 C]acetate, [3- 13 C] dl -phenylalanine, and 13 C-labeled compounds (WL, DWL and 3,5-diCQA) were detected by ultra-high-performance liquid chromatography-quadrupole time of flight mass spectrometry (HPLC-QTOF-MS). Analysis showed that the metabolic pathways operative of coumestans (WL and DWL) are derived from acetate and shikimate pathways, while that the phenylpropanoid (3,5-diCQA) biosynthesis is exclusively from shikimate pathway. Supplementary Information The online version contains supplementary material available at 10.1007/s11240-022-02342-0.

Hairy Root Cultures as a Source of Phenolic Antioxidants: Simple Phenolics, Phenolic Acids, Phenylethanoids, and Hydroxycinnamates

“…Control cultures contained 18.08 mg/g DW of the compound [ 147 ]. Lopes et al [ 148 ] investigated the biosynthesis of demethylwedelolactone, wedelolactone, and 3,5-DCQA in the HRs of E. prostrata . Feeding with [3- 13 C] DL -phenylalanine proved that in contrast to wedelolactones, 3,5-DCQA molecule originates entirely from the shikimate pathway.…”